The Effect of Biscuits Made From Pumpkin Seeds Flour on Serum Zinc Levels and Weight in Malnutrition Wistar Rats

Authors

  • Aminuddin Syam Department of Nutrition Science, Faculty of Public Health, Hasanuddin University, Makassar, Indonesia
  • Fitrah Khaerani Burhan Department of Nutrition Science, Faculty of Public Health, Hasanuddin University, Makassar, Indonesia
  • Veni Hadju Department of Nutrition Science, Faculty of Public Health, Hasanuddin University, Makassar, Indonesia
  • C. Citrakesumasari Department of Nutrition Science, Faculty of Public Health, Hasanuddin University, Makassar, Indonesia
  • Andi Muhammad Akhmar Department of Local Literature, Faculty of Cultural Science, Hasanuddin University, Makassar, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2020.4402

Keywords:

Functional food, Micronutrients, Nutritious biscuit, Nutritional status, Pumpkin seeds

Abstract

BACKGROUND: Serum zinc levels are found to be low in children of protein energy malnutrition globally. Pumpkin seed flour has a high zinc content of 6.88 mg/100 g, has been formulated biscuits made from pumpkin seed flour with a zinc content of 1.52 mg/100 g.

AIM: This study aims to determine the effect of giving biscuits made from pumpkin seed flour to serum zinc levels and body weight in malnutrition Wistar rats.

METHODS: This research is a true experiment with pre-post-test with control group design. Subjects were 28 male Wistar strain rats induced by fasting malnutrition for 3 days and then given biscuits with a zinc dose of 0.027 mg; 0.054 mg; and 0.081 mg/BW/day and in the control group without biscuits for 2 weeks. Serum zinc levels were examined using the atomic absorption spectrophotometry method. Data were analyzed using paired t-test and one-way ANOVA test.

RESULTS: Paired t-test results on serum zinc levels obtained p > 0.05 and on body weight obtained p < 0.05. One-way ANOVA test results on serum zinc levels after the intervention obtained p > 0.05 and at body weight obtained p < 0.05 followed by post hoc least significant difference test found that there were significant differences between the control group and the P1 group, a dose of 0.027 mg (p = 0.015) and P2 groups, doses of 0.054 mg (p = 0.012). Greater weight gain was found in the P1 group with an increase of 38.85%.

CONCLUSION: The provision of biscuits made from pumpkin seed flour has an effect on increasing body weight of malnutrition Wistar rats and has no effect on serum zinc levels, so further research is expected to use different doses.

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References

Best C, Neufingerl N, Van Geel L, van den Briel T, Osendarp S. The nutritional status of school-aged children: Why should we care? Food Nutr Bull 2010;31(3):400-17. https://doi. org/10.1177/156482651003100303 PMid:20973461

Kemenkes RI. Laporan Nasional Riskesdas. Jakarta: Badan Penelitian Dan Pengembangan Kesehatan Kementerian Kesehatan Republik Indonesia; 2018.

Müller O, Krawinkel M. Malnutrition and health in developing countries. CMAJ 2005;173(3):279-86. PMid:16076825

Kusudaryati DP. Kekurangan Asupan Besi dan Seng Sebagai Faktor Penyebab Stunting Pada Anak. Vol. 10. Profesi (Profesional Islam): Media Publikasi Penelitian; 2014. p. 57-62.

Mittal P, Dipti B. Role of zinc in malnutrition. Int J Gastroenterol Hepatol Transplant Nutr 2016;1(4):45-8. https://doi. org/10.26576/profesi.129

Brown KH, Hotz C. International zinc nutrition consultative group (IZiNCG) technical document #1. Assessment of the risk of zinc deficiency in populations and options for its control. Food Nutr Bull 2004;25(1):S99-203. https://doi. org/10.1177/156482650402500220 PMid:18046856

Paik I. Application of chelated minerals in animal production. Asian Austral J Anim Sci 2001;14(SPI):191-8.

Rahman MM, Tofail F, Wahed MA, Fuchs GJ, Baqui AH, Alvarez JO. Short-term supplementation with zinc and Vitamin A has no significant effect on the growth of undernourished Bangladeshi children. Am J Clin Nutr 2002;75(1):87-91. https:// doi.org/10.1093/ajcn/75.1.87 PMid:11756064

Brown KH, Peerson JM, Rivera J, Allen LH. Effect of supplemental zinc on the growth and serum zinc concentrations of prepubertal children: A meta-analysis of randomized controlled trials. Am J Clin Nutr 2002;75(6):1062-71. https://doi. org/10.1093/ajcn/75.6.1062 PMid:12036814

Kattelmann KK, Ho M, Specker BL. Effect of timing of introduction of complementary foods on iron and zinc status of formula fed infants at 12, 24, and 36 months of age. J Am Diet Assoc 2001;101(4):443-7. https://doi.org/10.1016/ s0002-8223(01)00114-6 PMid:11320951

Mulyaningsih TR. The concentration of Fe and Zn in agricutural, livestocks, and fishery food products using K0-AANI methods. J. Sains Teknol Nuklir Indones 2009;10(2):71-80. https://doi. org/10.21059/buletinpeternak.v11i1.1800.

Dhiman AK, Sharma KD, Attri S. Functional constitutents and processing of pumpkin: A review. J Food Sci Technol 2009;46(5):411-7.

Revathy MN, Sabitha N. Development, quality evaluation and popularization of pumpkin seed flour incorporated bakery products. Int J Food Nutr Sci 2013;2(2):40-5.

Syam A, Kurniati Y, Zaenal, Puspitasari N, Khaerani F. Nutrient content of pumkin seed (Cucurbita moschata Durch) Makassar City: The 1st International Conference on Nutrition and Public Health, Hotel Claro Makassar; 2019a.

Tona Z, Tafese B, Tefera B. Blending germinated maize, pumpkin pulp and its seed improves zinc and Vitamin A without compromising nutritive value and sensory attributes of local complementary food porridge. Food Public Health 2015;5(4):103-7.

Syam A, Zaenal YK, Aulia NA, Wati IP, Mansur MA. Development and biochemical analysis of pumpkin seed (Cucurbita moschata Durch) biscuits. Pak J Nutr 2019;18(8):743-6. https://doi. org/10.3923/pjn.2019.743.746

Todd WR, Elvehjem CA, Hart EB. Zinc in the nutrition of the rat. Am J Physiol Legacy Content 1933;107(1):146-56. https://doi. org/10.1152/ajplegacy.1933.107.1.146

De Benoist B, Darnton-Hill I, Davidsson L, Fontaine O, Hotz C. Conclusions of the joint WHO/UNICEF/IAEA/ IZiNCG interagency meeting on zinc status indicators. Food Nutr Bull 2007;28(Suppl 3):S480-4. https://doi. org/10.1177/15648265070283s306 PMid:17988008

Roohani N, Hurrell R, Kelishadi R, Schulin R. Zinc and its importance for human health: An integrative review. J Res Med Sci 2013;18(2):144-57.

Almatsier S. Prinsip Dasar Ilmu Gizi. Jakarta: PT Gramedia Pustaka Utama; 2009.

King JC, Shames DM, Woodhouse LR. Zinc homeostasis in humans. J Nutr 2000;130(5):1360S-6S. https://doi.org/10.1093/ jn/130.5.1360s PMid:10801944

Vakili R, Bakhsh My, Vahedian M, Mahmoudi M, Saeidi M, Vakili S. The effect of zinc supplementation on linear growth and growth factors in primary school children in the suburbs Mashhad, Iran. Int J Pediatr 2015;3(21):1-7.

Gropper S, Smith JL, Groff JL. Advanced Nutrition and Human Metabolism. Australia: Thomson; 2005.

Akbar B, Niloufar N, Abolfazl M, Lofollah S, Ali KQ, Soheyla V. Evaluation and comparison of zinc absorption level from 2-Alkyle 3-Hydroxy pyranon-zinc complexes and zinc sulfate in rat in vivo. Adv Biomed Res 2013;2(2):1-8. https://doi. org/10.4103/2277-9175.116432 PMid:24223392

Wessells KR, Ouédraogo ZP, Rouamba N, Hess SY, Ouédraogo JB, Brown KH. Short-term zinc supplementation with dispersible tablets or zinc sulfate solution yields similar positive effects on plasma zinc concentration of young children in Burkina Faso: A randomized controlled trial. J Pediatr 2012;160(1):129- 35. https://doi.org/10.1016/j.jpeds.2011.06.051 PMid:21871635

Lopes MM, De Brito NJ, De Medeiros Rocha ÉD, França MC, De Almeida MD, Brandão-Neto J. Nutritional assessment methods for zinc supplementation in prepubertal non zinc-deficient children. Food Nutr Res 2015;59(1):29733. https://doi. org/10.3402/fnr.v59.29733 PMid:26507491

Cunningham GJ, Klein GB. Text Book of Veterinary Physiology. 4th ed. Philadelphia, PA: Saunders, Elsevier; 2007. p. 328.

Maukina R, Wijayanti TR. Pemberian sirup zink berpengaruh terhadap perubahan berat badan pada balita kekurangan energi protein (KEP) Sedang. Care 2018;6(3):267-76. https:// doi.org/10.33366/cr.v6i3.1001

de Medeiros Rocha ÉD, de Brito NJ, Dantas MM, de Araújo Silva A, das Graças Almeida M, Brandão-Neto J. Effect of zinc supplementation on GH, IGF1, IGFBP3, OCN, and ALP in non-zinc-deficient children. J Am Coll Nutr 2015;34(4):290-9. https:// doi.org/10.1080/07315724.2014.929511 PMid:25759961

Chao HC, Chang YJ, Huang WL. Cut-off serum zinc concentration affecting the appetite, growth, and nutrition status of undernourished children supplemented with zinc. Nutr Clin Pract 2018;33(5):701-10. https://doi.org/10.1002/ncp.10079

PMid:29603391

Candra A. Pengaruh suplementasi seng dan zat besi terhadap berat badan dan tinggi badan balita. J Nutr Health 2017;5(1):37- 44. https://doi.org/10.14710/jnc.v6i4.18664

Agustian L, Sembiring T, Ariani A. Peran Zinkum Terhadap Pertumbuhan Anak. Sari Pediatr 2009;11(4):244-9. https://doi. org/10.14238/sp11.4.2009.244-9

Linder MC. Biokimia Nutrisi dan Metabolisme. Jakarta: Universitas Indonesia Press; 1992.

Alves CX, Vale SH, Dantas MM, Maia AA, Franca MC, Marchini JS, et al. Positive effects of zinc supplementation on growth, GH, IGF1, and IGFBP3 in eutrophic children. J Pediatr Endocrinol Metab 2012;25(9-10):881-7. https://doi.org/10.1515/ jpem-2012-0120

PMid:23426817

Tsalissavrina I, Wahono D, Handayani D. Pengaruh pemberian diet tinggi karbohidrat dibandingkan diet tinggi lemak terhadap kadar trigliserida dan HDL darah pada Rattus novergicus galur wistar. J Kedokteran Brawijaya 2013;22(2):80-9. https://doi. org/10.21776/ub.jkb.2006.022.02.5

Doherty CP, Sarkar MA, Shakur MS, Ling SC, Elton RA, Cutting WA. Zinc and rehabilitation from severe protein-energy malnutrition: Higher-dose regimens are associated with increased mortality. Am J Clin Nutr 1998;68(3):742-8. https:// doi.org/10.1093/ajcn/68.3.742 PMid:9734756

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Published

2020-04-25

How to Cite

1.
Syam A, Burhan FK, Hadju V, Citrakesumasari C, Akhmar AM. The Effect of Biscuits Made From Pumpkin Seeds Flour on Serum Zinc Levels and Weight in Malnutrition Wistar Rats. Open Access Maced J Med Sci [Internet]. 2020 Apr. 25 [cited 2024 Mar. 29];8(A):428-33. Available from: https://oamjms.eu/index.php/mjms/article/view/4402