Assessment of the −174G/C (rs1800795) and −572G/C (rs1800796) Interleukin 6 Gene Polymorphisms in Egyptian Patients with Rheumatoid Arthritis
DOI:
https://doi.org/10.3889/oamjms.2016.110Keywords:
Rheumatoid arthritis, Interleukin 6, IL6–174G/C, IL6–572G/C, gene polymorphismAbstract
AIM: This study aimed to investigate genotype and allele frequencies of −174 (rs1800795) and −572 (rs1800796) IL-6 promoter gene polymorphisms in Egyptian patients with rheumatoid arthritis (RA) in comparison to control group.
METHODS: The study was conducted on 198 Egyptian subjects (99 RA patients and 99 healthy control).The promoter region of the IL-6 gene was amplified by PCR using DNAs from patients and the controls, and their PCR products were digested by suitable enzymes.
RESULTS: No statistical differences were found in −572G/C genotype (P = 0.177) or allele (P = 0.147) frequencies between RA patients and controls. Significant differences were observed in −174G/C genotype (P ˂ 0.001) and allele (P ˂ 0.001) frequencies between RA patients and controls.
CONCLUSION: A significant association of IL-6 −174G/C gene polymorphism and RA in Egyptian population was found with significantly higher frequencies of GC and CC genotypes and C allele in RA patients compared to controls. No association was found between IL-6 −572G/C gene polymorphism and RA.Downloads
Metrics
Plum Analytics Artifact Widget Block
References
Gibofsky A. Overview of epidemiology, pathophysiology, and diagnosis of rheumatoid arthritis. Am J Manag Care. 2012;18(13 Suppl):S295-302. PMid:23327517
McInnes IB, Schett G. The pathogenesis of rheumatoid arthritis. N Engl J Med. 2011; 365(23):2205-2219. http://dx.doi.org/10.1056/NEJMra1004965 PMid:22150039 DOI: https://doi.org/10.1056/NEJMra1004965
Kurko, J, Besenyei T, Laki J et al. (2013) Genetics of Rheumatoid Arthritis - A Comprehensive Review. Clin Rev Allergy Immunol. 2013;45(2): 170–179. http://dx.doi.org/10.1007/s12016-012-8346-7 PMid:23288628 PMCid:PMC3655138 DOI: https://doi.org/10.1007/s12016-012-8346-7
Magyari L, Varszegi D, Kovesdi E et al. Interleukins and interleukin receptors in rheumatoid arthritis: Research, diagnostics and clinical implications. World J Orthop. 2014;18; 5(4): 516–536. DOI: https://doi.org/10.5312/wjo.v5.i4.516
Hashizume M, Mihara M. The roles of interleukin-6 in the pathogenesis of rheumatoid arthritis. Arthritis. 2011: 2011. DOI: https://doi.org/10.1155/2011/765624
Tanaka T, Ogata A, Kishimoto T. Targeting of interleukin-6 for the treatment of rheumatoid arthritis: a review and update. Rheumatology: Current Research. 2013;2014.
Zavaleta-Mu-iz SA, MartÃn-Márquez BT, Gonzalez-Lopez L, et al. Clinical Study The−174G/C and−572G/C Interleukin 6 Promoter Gene Polymorphisms in Mexican Patients with Rheumatoid Arthritis: A Case-Control Study. Clinical and Developmental Immunology. 2013; Article ID 959084. DOI: https://doi.org/10.1155/2013/959084
Arman A, Coker A, Sarioz, O et al. Lack of association between IL-6 gene polymorphisms and rheumatoid arthritis in Turkish population. Rheumatol Int. 2012;32:2199–2201. http://dx.doi.org/10.1007/s00296-011-2057-x PMid:21833517 DOI: https://doi.org/10.1007/s00296-011-2057-x
Li F, Xu J, Zheng J et al. Association between Interleukin-6 Gene Polymorphisms and Rheumatoid Arthritis in Chinese Han Population: A Case-Control Study and A Meta-analysis. Sci Rep. 2014; 4: 5714. http://dx.doi.org/10.1038/srep05714 DOI: https://doi.org/10.1038/srep05714
Aletaha D, Neogi T, Silman AJ et al. Rheumatoid arthritis classification criteria: an American College of Rheumatology/European League Against Rheumatism collaborative initiative. Arthritis Rheum. 2010; 62(9):2569–81. http://dx.doi.org/10.1002/art.27584 PMid:20872595 DOI: https://doi.org/10.1002/art.27584
Prevoo ML, Hof van't MA, Kuper HH et al. Modified disease activity scores that include twenty-eight-joint counts: development and validation in a prospective longitudinal study of patients with rheumatoid arthritis. Arthritis Rheum. 1995; 38: 44– 48. http://dx.doi.org/10.1002/art.1780380107 PMid:7818570 DOI: https://doi.org/10.1002/art.1780380107
Larsen A. How to apply Larsen score in evaluating radiographs of rheumatoid arthritis in long term studies. J Rheumatol. 1995; 22:1974–5. PMid:8992003
Singh PK, Chandra G, Bogra J et al. Association of interleukin-6 genetic polymorphisms with risk of OSCC in Indian population. Meta Gene. 2015; 4:142–151. http://dx.doi.org/10.1016/j.mgene.2015.03.002 PMid:26005639 PMCid:PMC4436510 DOI: https://doi.org/10.1016/j.mgene.2015.03.002
Yoshida Y and Tanaka T. Interleukin 6 and rheumatoid arthritis. Bio Med Research International. 2014;Article ID 698313. DOI: https://doi.org/10.1155/2014/698313
Lo SF, Huang CM, Lin HC et al. Cytokine (IL-6) and chemokine (IL-8) gene polymorphisms among rheumatoid arthritis patients in Taiwan Clinical and Experimental Rheumatology. 2008; 26(4):632–637.
Li X, Chai W, Ni M et al. The Effects of Gene Polymorphisms in Interleukin-4 andInterleukin-6 on the Susceptibility of Rheumatoid Arthritis ina Chinese Population. BioMed Research International. 2014;Article ID 265435. DOI: https://doi.org/10.1155/2014/265435
Kishimoto T. Interleukin-6: from basic science to medicine: 40 years inimmunology. Annu Rev Immunol. 2005; 23:1-21. http://dx.doi.org/10.1146/annurev.immunol.23.021704.115806 PMid:15771564 DOI: https://doi.org/10.1146/annurev.immunol.23.021704.115806
Fishman D, Faulds G, Jeffery R et al. The effect of novel polymorphisms in the interleukin-6 (IL-6) gene on IL-6 transcription and plasma IL-6 levels, and an association with systemic-onset juvenile chronic arthritis.J Clin Invest. 1998; 102(7):1369-1376. http://dx.doi.org/10.1172/JCI2629 PMid:9769329 PMCid:PMC508984 DOI: https://doi.org/10.1172/JCI2629
Ceccarelli F, Perricone C, Fabris M et al. Transforming growth factor β 869C/T and interleukin 6 -174G/C polymorphisms relate to the severity and progression of bone-erosive damage detected by ultrasound in rheumatoid arthritis.Arthritis Res Ther. 2011;13(4):R111. http://dx.doi.org/10.1186/ar3396 PMid:21740541 PMCid:PMC3239349 DOI: https://doi.org/10.1186/ar3396
Downloads
Published
How to Cite
Issue
Section
License
http://creativecommons.org/licenses/by-nc/4.0