Salivary Immunoglobulin Gene Expression in Patients with Caries

Authors

  • Gema Regina Guadarrama Santín
  • Angel Visoso Salgado
  • Norma Margarita Montiel Bastida
  • Isaías de la Rosa Gómez
  • Jonnathan Guadalupe Santillán Benítez
  • Hugo Mendieta Zerón http://orcid.org/0000-0003-3492-8950

DOI:

https://doi.org/10.3889/oamjms.2017.028

Keywords:

caries, gene expression, mixed dentition, permanent dentition, saliva.

Abstract

BACKGROUND: Immunoglobulins mediate the host’s humoral immune response are expressed in saliva.

AIM: To quantify the FcαR, FcγRIIB, and FcαμR gene expression in the saliva of Mexican patients with caries in mixed and permanent dentition.

SUBJECTS AND METHODS: This was a comparative cross-sectional study. mRNA was isolated from 200 μL of saliva following the RNA III Tissue Fresh-frozen protocol of the MagNA Pure LC Instrument 2.0 (Roche Diagnostics GmbH, Nederland BV) and the FcαR, FcαµR and FcγRIIB were quantified through TaqMan Assays.

RESULTS: One hundred individuals, 50 with mixed dentition and 50 with permanent dentition, were included in the study. Statistically, it was found a significant difference (p = 0.025) in the IgG (FcγRIIB) expression between the studied groups.

CONCLUSION: Although we confirmed the existence of FcαR, FcγRIIB and FcαμR gene expression in saliva, only a significant difference in the expression of FcγRIIB between the mixed dentition and permanent dentition was found.

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2017-03-03

How to Cite

1.
Guadarrama Santín GR, Visoso Salgado A, Montiel Bastida NM, de la Rosa Gómez I, Santillán Benítez JG, Mendieta Zerón H. Salivary Immunoglobulin Gene Expression in Patients with Caries. Open Access Maced J Med Sci [Internet]. 2017 Mar. 3 [cited 2024 Apr. 19];5(2):236-43. Available from: https://oamjms.eu/index.php/mjms/article/view/oamjms.2017.028

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Vol. 5 No. 2 (2017): Apr 15 (OAMJMS)

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D - Dental Sciences

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http://creativecommons.org/licenses/by-nc/4.0

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