A Comparative Study of Rat Lung Decellularization by Chemical Detergents for Lung Tissue Engineering

Hamid Tebyanian; Ali Karami; Ebrahim Motavallian; Jafar Aslani; Ali Samadikuchaksaraei; Babak Arjmand; Mohammad Reza Nourani

doi:10.3889/oamjms.2017.179

Authors

Hamid Tebyanian Division of Tissue Engineering and Regenerative Medicine, Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran; Research Center for Prevention of Oral and Dental Disease, Baqiyatallah University of Medical Sciences, Tehran, Iran
Ali Karami Division of Tissue Engineering and Regenerative Medicine, Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran; Research Center for Prevention of Oral and Dental Disease, Baqiyatallah University of Medical Sciences, Tehran
Ebrahim Motavallian Department of General Surgery, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran
Jafar Aslani Department of General Surgery, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran
Ali Samadikuchaksaraei Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran; Department of Tissue Engineering & Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran
Babak Arjmand Cell Therapy and Regenerative Medicine Research Center, Endocrinology and Metabolism Cellular-Molecular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
Mohammad Reza Nourani Cell Therapy and Regenerative Medicine Research Center, Endocrinology and Metabolism Cellular-Molecular Sciences Institute, Tehran University of Medical Sciences, Tehran

DOI:

https://doi.org/10.3889/oamjms.2017.179

Keywords:

Decellularization, CHAPS, SDS, SDC and Triton X-100

Abstract

BACKGROUND: Lung disease is the most common cause of death in the world. The last stage of pulmonary diseases is lung transplantation. Limitation and shortage of donor organs cause to appear tissue engineering field. Decellularization is a hope for producing intact ECM in the development of engineered organs.

AIM: The goal of the decellularization process is to remove cellular and nuclear material while retaining lung three-dimensional and molecular proteins. Different concentration of detergents was used for finding the best approach in lung decellularization.

MATERIAL AND METHODS: In this study, three-time approaches (24, 48 and 96 h) with four detergents (CHAPS, SDS, SDC and Triton X-100) were used for decellularizing rat lungs for maintaining of three-dimensional lung architecture and ECM protein composition which have significant roles in differentiation and migration of stem cells This comparative study determined that variable decellularization approaches can cause significantly different effects on decellularized lungs.

RESULTS: Results showed that destruction was increased with increasing the detergent concentration. Single detergent showed a significant reduction in maintaining of three-dimensional of lung and ECM proteins (Collagen and Elastin). But, the best methods were mixed detergents of SDC and CHAPS in low concentration in 48 and 96 h decellularization.

CONCLUSION: Decellularized lung tissue can be used in the laboratory to study various aspects of pulmonary biology and physiology and also, these results can be used in the continued improvement of engineered lung tissue.

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