Rapid Identification of Aspergillus Fumigatus Using Î’eta-Tubulin and RodletA Genes

Majid Zarrin; Zeinab Rashidnia; Sama Faramarzi; Lida Harooni

doi:10.3889/oamjms.2017.203

Authors

Majid Zarrin Health Research Institute, Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz
Zeinab Rashidnia Department of Medical Mycology, Medical School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz
Sama Faramarzi Department of Medical Mycology, Medical School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz
Lida Harooni Department of Medical Mycology, Medical School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz

DOI:

https://doi.org/10.3889/oamjms.2017.203

Keywords:

Î²-tubulin, rodletA, Aspergillus fumigatus

Abstract

AIM: The main purpose of the present study was to test the Î²-tubulin and rodletA genes for rapid identification of Aspergillus fumigatus.

MATERIALS AND METHODS: Fifty-one A. fumigatus strains including environmental, clinical and reference isolates were tested in this research. PCR was carried out based on Î²tub and rodA partial gene sequences.

RESULTS: A 198 bp DNA fragment was obtained using Î²tub gene. PCR amplification of the rodA gene resulted in a 313 bp band. The Î²tub and rodA genes PCR products exhibited a 100% homology with the associated sequences in the GenBank.

CONCLUSION: In the present study, we used a PCR approach that was able to discriminate A. fumigatus from other related species within the section Fumigati.

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