In Vitro Evaluation of the Sensitivity of a Hyaluronic Acid PEG Cross-Linked to Bovine Testes Hyaluronidase

Authors

  • Nicola Zerbinati Universita degli Studi dell'Insubria Dipartimento di Scienze Chirurgiche e Morfologiche, Varese
  • Torello Lotti University G. Marconi of Rome, Dermatology and Venereology, Rome
  • Damiano Monticelli Universita degli Studi dell'Insubria Dipartimento di Scienza e Alta Tecnologia, Como
  • Virginia Martina Matex Lab, Brindisi
  • Giovanna Cipolla Università di Pavia - Centro Medico Polispecialistico, Pavia
  • Edoardo D'Este Università di Pavia - Centro Medico Polispecialistico, Pavia
  • Alberto Calligaro University of Pavia - Department of Public Health, Experimental and Forensic Medicine, Pavia
  • Roberto Mocchi UB - CARE S.r.l. - Spin - off University of Pavia, Pavia
  • Cristina Maccario UB - CARE S.r.l. - Spin - off University of Pavia, Pavia
  • Sabrina Sommatis UB - CARE S.r.l. - Spin - off University of Pavia, Pavia
  • Jacopo Lotti University of Rome “G. Marconi” - Department of Nuclear, Subnuclear and Radiation Physics, Rome
  • Uwe Wollina Städtisches Klinikum Dresden, Department of Dermatology and Venereology, Dresden, Sachsen
  • Georgi Tchernev Medical Institute of Ministry of Interior (MVR), Department of Dermatology, Venereology and Dermatologic Surgery, Sofia
  • Katlein França University of Miami School of Medicine, Department of Psychiatry & Behavioral Sciences, Miami, Florida

DOI:

https://doi.org/10.3889/oamjms.2018.046

Keywords:

Hyaluronic acid, Sensitivity to hyaluronidase, N-acetyl-D-glucosamine, Neauvia Intense, Poly ethylene glycol

Abstract

Neauvia Intense is biocompatible, injectable hyaluronic acid (HA) filler PEG cross-linked for facial soft-tissue augmentation that provides volume to tissues. The aim of the present study is to evaluate the sensitivity of Neauvia Intense in hyaluronidase from bovine testes in a time-course analysis. The test is based on the colourimetric determination of the N-acetyl – D - glucosamine (NAG) released by the hyaluronidase in standardised conditions. The in vitro conditions involve the treatment of Neauvia Intense with a known concentration of the enzyme (6080U/ml). The NAG content was determined at different times to assess the kinetics of the degradation (1h, 3h, 6h, 24h, 48h, 72h, 120h, and 168h); the Ehrlich’s reagent was used for the colourimetric quantification, by the method described by Reissing and colleagues. The intensity of the violet colour developed after the chemical reaction was proportional to the NAG present in each sample. A microplate reader at 585 nm read the absorbance. The amount of NAG released by the product was proportional to the time of incubation with bovine hyaluronidase, reaching a plateau after 168 hours.

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Published

2018-01-21

How to Cite

1.
Zerbinati N, Lotti T, Monticelli D, Martina V, Cipolla G, D’Este E, Calligaro A, Mocchi R, Maccario C, Sommatis S, Lotti J, Wollina U, Tchernev G, França K. In Vitro Evaluation of the Sensitivity of a Hyaluronic Acid PEG Cross-Linked to Bovine Testes Hyaluronidase. Open Access Maced J Med Sci [Internet]. 2018 Jan. 21 [cited 2021 Apr. 11];6(1):20-4. Available from: https://oamjms.eu/index.php/mjms/article/view/oamjms.2018.046

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