Isothermal PCR for Feasible Molecular Diagnosis of Primary Toxoplasmosis in Women Recently Experienced Spontaneous Abortion

Authors

  • Amany A. Abd El Aal Department of Medical Parasitology, Faculty of Medicine, Cairo University, Cairo; Department of Medical Parasitology, Armed Forces College of Medicine, Cairo
  • Reham K. Nahnoush Department of Medical Parasitology, Faculty of Medicine, Cairo University, Cairo
  • Marwa A. Elmallawany Department of Medical Parasitology, Faculty of Medicine, Cairo University, Cairo
  • Walid S. El-Sherbiny Department of Obstetrics & Gynecology, Faculty of Medicine, Cairo University, Cairo
  • Mohamed S. Badr Department of Molecular Biology, Medical Research Center, Faculty of Medicine, Ain Shams University, Cairo
  • Ghada M. Nasr Department of Molecular Diagnostics, Genetic Engineering and Biotechnology Research Institute, City University, Cairo

DOI:

https://doi.org/10.3889/oamjms.2018.227

Keywords:

Isothermal, LAMP, Toxoplasma, qPCR, Abortion

Abstract

AIM: The current study aimed to assess the practicability of a simple loop-mediated isothermal amplification (LAMP) about real-time quantitative PCR to diagnose primary toxoplasmosis among high-risk pregnant women.

METHODS: Cloned Toxoplasma samples were used to calculate the analytical sensitivity while specificity was assessed using pooled DNA samples extracted from other parasitic stages.

RESULTS: Both techniques showed 100% sensitivity and specificity and then applied to detect recent Toxoplasma infection in peripheral blood of 77 IgG negative women out of a total 139 women lately experienced spontaneous abortion. The 2 techniques obtained positive results in 8 samples confirming primary toxoplasmosis.

CONCLUSION: Generally, LAMP assay is a simple, cost-effective molecular technique can be completed in less than half an hour to diagnose primary Toxoplasma infection. The technique can be applied in a minimally equipped laboratory by ordinary workers to screen the vulnerable groups. Further analysis using larger samples with the quantitative approach is recommended to confirm the sensitivity of this emergent molecular technique.

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Published

2018-06-10

How to Cite

1.
Abd El Aal AA, Nahnoush RK, Elmallawany MA, El-Sherbiny WS, Badr MS, Nasr GM. Isothermal PCR for Feasible Molecular Diagnosis of Primary Toxoplasmosis in Women Recently Experienced Spontaneous Abortion. Open Access Maced J Med Sci [Internet]. 2018 Jun. 10 [cited 2024 Mar. 29];6(6):982-7. Available from: https://oamjms.eu/index.php/mjms/article/view/oamjms.2018.227

Issue

Section

A - Basic Science