Nano Hydroxyapatite & Mineral Trioxide Aggregate Efficiently Promote Odontogenic Differentiation of Dental Pulp Stem Cells

Ahmed Khaled Hanafy; Souzy F. Shinaishin; Ghada Nour Eldeen; Riham Mohammed Aly

doi:10.3889/oamjms.2018.368

Authors

Ahmed Khaled Hanafy Department of Oral Biology, Faculty of Dentistry, Egyptian Russian University in Cairo
Souzy F. Shinaishin Department of Oral Biology, Faculty of Dentistry, Ain Shams University, Cairo
Ghada Nour Eldeen Department of Molecular Genetics and Enzymology, National Research Centre, Cairo, Egypt; Stem Cell Research Group, Medical Research Centre of Excellence, National Research Centre, Cairo
Riham Mohammed Aly Basic Dental Science Department, Oral & Dental Research Division, National Research Centre, Cairo, Egypt; Stem Cell Laboratory, Centre of Excellence for Advanced Sciences, National Research Centre, Cairo

DOI:

https://doi.org/10.3889/oamjms.2018.368

Keywords:

Dental Pulp Stem Cells, Odontogenic Differentiation, Mineral trioxide aggregate, Nano hydroxyapatite

Abstract

BACKGROUND: There has been an urge to shift from conventional therapies to the more promising regenerative strategy since conventional treatment relies on synthetic materials to fill defects and replace missing tissues, lacking the ability to restore the tissuesâ€™ physiological architecture and function.

AIM: The present study focused on the assessment of the role of two commonly used biomaterials namely; mineral trioxide aggregate (MTA) and nano hydroxy-apatite as promoters of odontogenic differentiation of dental pulp stem cells (DPSCs).

METHODS: DPSCs were isolated, cultured in odontogenic media and divided into three groups; control group, MTA group and nanohydroxyapatite group. Odontogenic differentiation was assessed by tracing genes characteristic of different stages of odontoblasts via qRT-PCR. Calcific nodules formation was evaluated by Alizarin red staining.

RESULTS: Results demonstrated that both MTA and nanohydroxyapatite were capable of enhancing odontogenic differentiation of DPSCs.

CONCLUSION: Nano hydroxyapatite was found to have a higher promoting effect. However, in the absence of an odontogenic medium, MTA and nanohydroxyapatite could not enhance the odontogenic differentiation of DPSCs.