The Role of TNF-α induced MSCs on Suppressive Inflammation by Increasing TGF-β and IL-10

Authors

  • Agung Putra Stem Cell and Cancer Research of Medical Faculty, UNISSULA, Semarang, Indonesia; Departement of Biomedical Postgraduate of Medical Faculty, UNISSULA, Semarang; Department of Pathological Anatomy of Medical Faculty UNISSULA, Semarang http://orcid.org/0000-0003-4261-9437
  • Fatkhan Baitul Ridwan Student of Medical Faculty, UNISSULA, Semarang
  • Allisha Irwaniyanti Putridewi Student of Medical Faculty, UNISSULA, Semarang
  • Azizah Retno Kustiyah
  • Ken Wirastuti Departement of Neurology of Medical Faculty, UNISSULA, Semarang
  • Nur Anna Chalimah Sadyah Departement of Internal Medicine Science of Medical Faculty, UNISSULA, Semarang
  • Ika Rosdiana Departement of Medical Rehabilitation of Medical Faculty, UNISSULA, Semarang
  • Delfitri Munir Pusat Unggulan Inovasi (PUI) Stem Cell of Medical Faculty, USU, Medan

DOI:

https://doi.org/10.3889/oamjms.2018.404

Keywords:

TNF-α, MSC, TGF-β, IL-10

Abstract

BACKGROUND: Mesenchymal stem cells (MSCs) may serve as immunoregulators by producing various anti-inflammatory molecules. Under sufficient level of TNF-α, MSCs become activated and adopt immune-suppressive phenotype (MSCs type-2) by releasing various anti-inflammatory molecule including TGF-β and IL-10. However, the ability of MSC itself to produce IL-10 under TNF-α stimulation and the correlation of TGF-β production of MSCs to IL-10 level remains to be elucidated.

AIM: In this study, MSCs were activated with various TNF-α doses to determine the increase of IL-10 and TGF-β level as well as its correlation.

MATERIAL AND METHODS: This study used post-test only control group design, by using 3 study groups, consist of 1 control (C) and 2 treatments (T) (TNF-α = 5 and 10 ng/mL) with triplicate induced in MSC for 24 hours, then the levels of IL-10 and TGF-β were measured by using ELISA assay.

RESULTS: The results of this study showed a significant increase of TGF-β and IL-10 levels (p < 0.05) at TNF-α 5 and 10 ng/mL dose of TNF-α. Moreover, there was a significant negative correlation between TGF-β and IL-10 level on 5 and 10 ng/mL dose TNF-α treatment.

CONCLUSION: Based on our study, we conclude that the 5 ng/mL dose of TNF-α is a sufficient dose for MSCs to suppress the inflammatory milieu. The higher increase of TGF beta is due to the controlled inflammation by IL-10.

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Published

2018-10-04

How to Cite

1.
Putra A, Ridwan FB, Putridewi AI, Kustiyah AR, Wirastuti K, Sadyah NAC, Rosdiana I, Munir D. The Role of TNF-α induced MSCs on Suppressive Inflammation by Increasing TGF-β and IL-10. Open Access Maced J Med Sci [Internet]. 2018 Oct. 4 [cited 2021 Apr. 12];6(10):1779-83. Available from: https://oamjms.eu/index.php/mjms/article/view/oamjms.2018.404

Issue

Section

A - Basic Science

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