The Role of TNF-Î± induced MSCs on Suppressive Inflammation by Increasing TGF-Î² and IL-10
Keywords:TNF-Î±, MSC, TGF-Î², IL-10
BACKGROUND: Mesenchymal stem cells (MSCs) may serve as immunoregulators by producing various anti-inflammatory molecules. Under sufficient level of TNF-Î±, MSCs become activated and adopt immune-suppressive phenotype (MSCs type-2) by releasing various anti-inflammatory molecule including TGF-Î² and IL-10. However, the ability of MSC itself to produce IL-10 under TNF-Î± stimulation and the correlation of TGF-Î² production of MSCs to IL-10 level remains to be elucidated.
AIM: In this study, MSCs were activated with various TNF-Î± doses to determine the increase of IL-10 and TGF-Î² level as well as its correlation.
MATERIAL AND METHODS: This study used post-test only control group design, by using 3 study groups, consist of 1 control (C) and 2 treatments (T) (TNF-Î± = 5 and 10 ng/mL) with triplicate induced in MSC for 24 hours, then the levels of IL-10 and TGF-Î² were measured by using ELISA assay.
RESULTS: The results of this study showed a significant increase of TGF-Î² and IL-10 levels (p < 0.05) at TNF-Î± 5 and 10 ng/mL dose of TNF-Î±. Moreover, there was a significant negative correlation between TGF-Î² and IL-10 level on 5 and 10 ng/mL dose TNF-Î± treatment.
CONCLUSION: Based on our study, we conclude that the 5 ng/mL dose of TNF-Î± is a sufficient dose for MSCs to suppress the inflammatory milieu. The higher increase of TGF beta is due to the controlled inflammation by IL-10.
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