The Role of TNF-Î± induced MSCs on Suppressive Inflammation by Increasing TGF-Î² and IL-10

Agung Putra; Fatkhan Baitul Ridwan; Allisha Irwaniyanti Putridewi; Azizah Retno Kustiyah; Ken Wirastuti; Nur Anna Chalimah Sadyah; Ika Rosdiana; Delfitri Munir

doi:10.3889/oamjms.2018.404

Authors

Agung Putra Stem Cell and Cancer Research of Medical Faculty, UNISSULA, Semarang, Indonesia; Departement of Biomedical Postgraduate of Medical Faculty, UNISSULA, Semarang; Department of Pathological Anatomy of Medical Faculty UNISSULA, Semarang http://orcid.org/0000-0003-4261-9437
Fatkhan Baitul Ridwan Student of Medical Faculty, UNISSULA, Semarang
Allisha Irwaniyanti Putridewi Student of Medical Faculty, UNISSULA, Semarang
Azizah Retno Kustiyah
Ken Wirastuti Departement of Neurology of Medical Faculty, UNISSULA, Semarang
Nur Anna Chalimah Sadyah Departement of Internal Medicine Science of Medical Faculty, UNISSULA, Semarang
Ika Rosdiana Departement of Medical Rehabilitation of Medical Faculty, UNISSULA, Semarang
Delfitri Munir Pusat Unggulan Inovasi (PUI) Stem Cell of Medical Faculty, USU, Medan

DOI:

https://doi.org/10.3889/oamjms.2018.404

Keywords:

TNF-Î±, MSC, TGF-Î², IL-10

Abstract

BACKGROUND: Mesenchymal stem cells (MSCs) may serve as immunoregulators by producing various anti-inflammatory molecules. Under sufficient level of TNF-Î±, MSCs become activated and adopt immune-suppressive phenotype (MSCs type-2) by releasing various anti-inflammatory molecule including TGF-Î² and IL-10. However, the ability of MSC itself to produce IL-10 under TNF-Î± stimulation and the correlation of TGF-Î² production of MSCs to IL-10 level remains to be elucidated.

AIM: In this study, MSCs were activated with various TNF-Î± doses to determine the increase of IL-10 and TGF-Î² level as well as its correlation.

MATERIAL AND METHODS: This study used post-test only control group design, by using 3 study groups, consist of 1 control (C) and 2 treatments (T) (TNF-Î± = 5 and 10 ng/mL) with triplicate induced in MSC for 24 hours, then the levels of IL-10 and TGF-Î² were measured by using ELISA assay.

RESULTS: The results of this study showed a significant increase of TGF-Î² and IL-10 levels (p < 0.05) at TNF-Î± 5 and 10 ng/mL dose of TNF-Î±. Moreover, there was a significant negative correlation between TGF-Î² and IL-10 level on 5 and 10 ng/mL dose TNF-Î± treatment.

CONCLUSION: Based on our study, we conclude that the 5 ng/mL dose of TNF-Î± is a sufficient dose for MSCs to suppress the inflammatory milieu. The higher increase of TGF beta is due to the controlled inflammation by IL-10.