The Effect of Puguntano Leaf Extract (Curanga Fel - Terrae Merr.) On P38 Mapk Levels and Glut-4 Expression in Type 2 Diabetic Rat Muscle

Authors

  • Santi Syafril Department of Internal Medicine, Faculty of Medicine, Universitas Sumatera Utara, H. Adam Malik General Hospital, Medan, Indonesia
  • Dharma Lindarto Department of Internal Medicine, Faculty of Medicine, Universitas Sumatera Utara, H. Adam Malik General Hospital, Medan, Indonesia
  • Aznan Lelo Department of Pharmacology and Therapeutic, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
  • Rosita Juwita Sembiring Department of Clinical Pathology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
  • Asman Manaf Department of Internal Medicine, Faculty of Medicine, Universitas Andalas, Dr M. Djamil General Hospital, Padang, Indonesia
  • Imam Budi Putra Department Dermatovenereology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
  • Poppy Anjelisa Zaitun Hasibuan Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
  • Erna Mutiara Department of Biostatistics, Faculty of Public Health, University of Sumatera Utara, Medan, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2019.165

Keywords:

Puguntano, p38 MAPK, GLUT-4, T2DM

Abstract

BACKGROUND: Puguntano (Curanga feel-terrae Merr.) contains flavonoids, saponins, tannins, and steroids/ terpenoids which improved post-receptor insulin signalling in rats model of type 2 diabetes mellitus (T2DM).

AIM: This study aimed to determine the effect of puguntano leaf extract on p38 mitogen-activated protein kinase (MAPK) levels and glucose transporter-4 (GLUT-4) expression in diabetic rats muscle.

METHODS: Forty-eight male Wistar rats had T2DM induced using a combination of feeding a high-fat diet for 5 weeks and multiple intraperitoneal injections of low-dose streptozotocin (30 mg/kg). The diabetic rats were randomly divided into control and treatment groups, and 200 mg/kg/day puguntano extract was administered orally for 10 days to treatment group. Subsequently, p38 MAPK levels were measured by Sandwich Elisa and plasma membrane GLUT-4 expression was evaluated by Immunohistochemistry in their gastrocnemius muscles.

RESULTS: There were significantly higher p38 MAPK levels and GLUT-4 expression in the treatment group than in the control group.

CONCLUSION: These data suggest that a puguntano leaf extract can improve post-receptor insulin signalling by enhancing p38 MAPK levels and GLUT-4 expression in a rat model of T2DM.

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Published

2019-02-26

How to Cite

1.
Syafril S, Lindarto D, Lelo A, Sembiring RJ, Manaf A, Putra IB, Hasibuan PAZ, Mutiara E. The Effect of Puguntano Leaf Extract (Curanga Fel - Terrae Merr.) On P38 Mapk Levels and Glut-4 Expression in Type 2 Diabetic Rat Muscle. Open Access Maced J Med Sci [Internet]. 2019 Feb. 26 [cited 2021 Jun. 25];7(4):521-5. Available from: https://oamjms.eu/index.php/mjms/article/view/oamjms.2019.165

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Section

A - Basic Science

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