A New Solid-Phase Extraction Method for Determination of Pantoprazole in Human Plasma Using High-Performance Liquid Chromatography

Authors

  • Dragica Zendelovska Institute of Preclinical and Clinical Pharmacology and Toxicology, Medical Faculty, Ss. Cyril and Methodius University of Skopje, 50 Divizija 6, 1000 Skopje, Republic of Macedonia
  • Emilija Atanasovska Institute of Preclinical and Clinical Pharmacology and Toxicology, Medical Faculty, Ss. Cyril and Methodius University of Skopje, 50 Divizija 6, 1000 Skopje, Republic of Macedonia
  • Kalina Gjorgjievska Institute of Preclinical and Clinical Pharmacology and Toxicology, Medical Faculty, Ss. Cyril and Methodius University of Skopje, 50 Divizija 6, 1000 Skopje, Republic of Macedonia
  • Kristina Pavlovska Institute of Preclinical and Clinical Pharmacology and Toxicology, Medical Faculty, Ss. Cyril and Methodius University of Skopje, 50 Divizija 6, 1000 Skopje, Republic of Macedonia
  • Krume Jakjovski Institute of Preclinical and Clinical Pharmacology and Toxicology, Medical Faculty, Ss. Cyril and Methodius University of Skopje, 50 Divizija 6, 1000 Skopje, Republic of Macedonia
  • Dimce Zafirov Institute of Preclinical and Clinical Pharmacology and Toxicology, Medical Faculty, Ss. Cyril and Methodius University of Skopje, 50 Divizija 6, 1000 Skopje, Republic of Macedonia
  • Jasmina Trojacanec Institute of Preclinical and Clinical Pharmacology and Toxicology, Medical Faculty, Ss. Cyril and Methodius University of Skopje, 50 Divizija 6, 1000 Skopje, Republic of Macedonia

DOI:

https://doi.org/10.3889/oamjms.2019.237

Keywords:

Pantoprazole, Solid-phase extraction, High-performance liquid chromatography, Human plasma

Abstract

BACKGROUND: A new simple, selective and accurate high-performance liquid chromatographic (HPLC) method utilising solid-phase extraction for the determination of pantoprazole in human plasma samples has been developed.
AIM: The purpose of this paper was developing a new HPLC method suitable for the determination of pantoprazole in plasma samples, which enables simple and rapid isolation and concentration of the analysed drug.
METHODS: The chromatographic separation was accomplished on a LiChroCart LiChrospher 60 RP select B column using a mobile phase composed of 0.2 % (V/V) water solution of triethylamine (pH 7) and acetonitrile (58:42, V/V) followed by UV detection was at 280 nm. The solid-phase extraction method using LiChrolut RP-18 (200 mg, 3 ml) was applied to the obtained good separation of investigated drug from endogenous plasma components. Best results were achieved when plasma samples were buffered with 0.1 mol/L KH2PO4 (pH 9) before extraction, eluted and reconstituted with acetonitrile and 0.001 mol/L NaOH after extraction, respectively.
RESULTS: The standard calibration curves showed good linearity within the range of 25.0-4000.0 ng/mL with a correlation coefficient greater than 0.996. Retention times of pantoprazole and internal standard, lansoprazole was 4.1 and 6.0 min respectively. The limit of quantification was 25.0 ng/mL. For intra- and inter-day precision relative standard deviations ranged from 4.2 to 9.3%. The relative errors for stability investigations were ranged from 0.12 to -10.5%.
CONCLUSION: This method has good precision and accuracy and was successfully applied to the pharmacokinetic and bioequivalence study of 40 mg pantoprazole in healthy volunteers.

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References

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Published

2019-06-15

How to Cite

1.
Zendelovska D, Atanasovska E, Gjorgjievska K, Pavlovska K, Jakjovski K, Zafirov D, Trojacanec J. A New Solid-Phase Extraction Method for Determination of Pantoprazole in Human Plasma Using High-Performance Liquid Chromatography. Open Access Maced J Med Sci [Internet]. 2019 Jun. 15 [cited 2021 Jul. 31];7(11):1757-61. Available from: https://oamjms.eu/index.php/mjms/article/view/oamjms.2019.237

Issue

Section

A - Basic Science

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