Exercise Intensity Alter Insulin Receptor Gene Expression in Diabetic Type - 2 Rat Model

Yetty Machrina; Ambrosius Purba; Dharma Lindarto; Ani Melani Maskoen

doi:10.3889/oamjms.2019.425

Authors

Yetty Machrina Department of Physiology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
Ambrosius Purba Department of Physiology, Faculty of Medicine, Universitas Padjajaran, Bandung, Indonesia
Dharma Lindarto Department of Internal Medicine, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
Ani Melani Maskoen Department of Oral Biology, Faculty of Dentistry, Universitas Padjajaran, Bandung, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2019.425

Keywords:

IR gene, moderate continuous training, severe continuous training, DM type-2

Abstract

AIM: To analyse the differential expression of the insulin receptor (IR) gene between moderate continuous and severe continuous training in the T2DM rat model.

METHODS: This was an experimental study. Healthy male Wistar was used in this study, which divided into sedentary, moderate continuous training, and severe continuous training. Treated groups were assigned to run on the treadmill three times a week for eight weeks consequently.

RESULTS: The result shown that expression of mRNA IR gene in treated groups decline compared to control. There was a difference mRNA IR gene expression after eight weeks of exercise between MCT and control, SCT and control so are MCT and SCT. IR expression on skeletal muscle in treated groups was different compared with control. The distribution of IR on skeletal muscles in treatment groups was significantly increased compared control, but there was no significant difference distribution between MCT and SCT. HOMA-IR post-test in SCT was lower than MCT but FBG post-test lower in MCT than SCT.

CONCLUSION: The intensity of exercise makes a difference in IR gene expression between moderate continuous training and severe continuous training after eight weeks of assigned exercise in T2DM rat models.