Antiarthritic Activity of Achyranthes Aspera on Formaldehyde - Induced Arthritis in Rats

Velmurugan Chinnasamy; Vetriselvan Subramaniyan; Sarath Chandiran; Saminathan Kayarohanam; Dhanapal Ð¡hidambaram Kanniyan; Venkata Sathya Saiappala Raju Velaga; Shafiulla Muhammad

doi:10.3889/oamjms.2019.559

Authors

Velmurugan Chinnasamy Department of Chemistry, Toxicology Unit, College of Natural Sciences, Arba Minch University, Ethiopia
Vetriselvan Subramaniyan Department of Pharmacology, Faculty of Medicine, MAHSA University, Malaysia
Sarath Chandiran School of Pharmacy, Sri Balaji Vidyapeeth Deemed University, India
Saminathan Kayarohanam Faculty of Bio-economy and Health Sciences, Geomatika University College Kuala Lumpur, Malaysia
Dhanapal Ð¡hidambaram Kanniyan Department of Pharmacy, Annamalai University, India
Venkata Sathya Saiappala Raju Velaga Faculty of Pharmacy, MAHSA University, Malaysia
Shafiulla Muhammad Department of Pharmacology, Sri Krishna Chaithanya College of pharmacy, Madanapalle, Andhra Pradesh, India

DOI:

https://doi.org/10.3889/oamjms.2019.559

Keywords:

Achyranthesaspera, Formaldehyde, Arthritis, Anti-arthritis

Abstract

AIM: To determine the ameliorative potential of aqueous extract of Achyranthesaspera(AEAA) against arthritis using swiss albino mice and Wistar rats, and its possible mechanism of action.

METHODS: Swiss albino mice (25-30 g) and Wistar rats (150-180 g) under standard controlled conditions (24 Â± 2Â°C, 50-70 humidity and 12 h light/dark cycle). The groups were divided into 6 groups (n = 6/group) and assigned as control, negative control, standard and, formaldehyde supplemented with two different test dose groups of A. aspera for 4 weeks. Arthritis induced by subplantar administration of 0.1 ml formaldehyde (2% v/v) into the left hind paw in all groups except normal control. Arthritis was assessed using serum Hb, ESR, paw volume, joint diameter, radiological and histopathological investigation.

RESULTS: Oral administration of AEAA shown a significant (p < 0.01) dose-dependent protection against formaldehyde induced arthritis. At 21st day, A.asperashown an inhibition of paw volume in the different doses of 250 mg/kg and 500 mg/kg were found to be 30% and, 38.33% respectively. At 14th day the joint swelling was found to be 27.2% and 36.36 respectively. Diclofenac (10 mg/kg) had an effect of 36.61% inhibition of arthritis and joint swelling at 21st and 14th day.

CONCLUSION: Thus, the present study revealed that the aqueous extract of A. aspera offered significant protection against arthritis and joint inflammation.