Royal Jelly (Bee Product) Decreases Inflammatory Response in Wistar Rats Induced with Ultraviolet Radiation

Authors

  • Fatmawati Fatmawati Department of Biochemistry, Faculty of Medicine, Universitas Sriwijaya, Palembang, Indonesia
  • Erizka Erizka Department of Microbiology, Faculty of Medicine, Universitas Sriwijaya, Palembang, Indonesia
  • Rachmat Hidayat Department of Biology, Faculty of Medicine, Universitas Sriwijaya, Palembang, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2019.704

Keywords:

Royal jelly, Ultraviolet radiation, Nrf 2, NF-κB, TNF alpha

Abstract

BACKGROUND: Ultraviolet (UV) radiation damages human skin by triggering various types of cellular damage, several main factors involved are nuclear-related factor 2 (Nrf2), nuclear factor kappa-light-chain-enhancer of activated B cells (nF-kB) and pro-inflammatory cytokine, TNF alpha. Royal jelly (RJ) possesses the effect of protecting DNA and tissue against oxidative damage.

AIM: This study aimed to assess the efficacy of RJ as a protector of ultraviolet radiation, by assessing endogenous anti-oxidant expression (Nrf2), transcription factors (Nf-kB) and proinflammatory cytokines (TNF alpha).

METHODS: This study was an experimental study with post-test control group design. Thirty Wistar rats were induced by exposing 40 Watt UV-B lamps for 2 hours/day in 14 days. The rats were grouped into groups with RJ cream application with doses of 2.5%, 5%, and 10%, negative control with vaseline, and normal control. Examination of Nrf2 and NF-kB levels was carried out by ELISA. Quantitative analysis to obtain the percentage of TNF alpha expression on the tissue was entered into the ImageJ® program. Bivariate analysis was carried out by the T-test.

RESULTS: Nrf2 levels elevated following the increase of RJ dose, with the highest level was at RJ 10%. Nf-kB levels decreased following the increase of RJ dose, with the lowest level was at RJ 10%. TNF alpha expression was reduced in groups of RJ in various doses. Increased dose resulted in a more diminished level of TNF alpha.

CONCLUSION: Royal jelly cream application protected the skin from UV radiation by increasing cellular antioxidants and suppressing inflammatory cascade.

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Published

2019-08-29

How to Cite

1.
Fatmawati F, Erizka E, Hidayat R. Royal Jelly (Bee Product) Decreases Inflammatory Response in Wistar Rats Induced with Ultraviolet Radiation. Open Access Maced J Med Sci [Internet]. 2019 Aug. 29 [cited 2022 Oct. 1];7(17):2723-7. Available from: https://oamjms.eu/index.php/mjms/article/view/oamjms.2019.704

Issue

Section

A - Basic Science

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