The Activity of Hydrolyzed Virgin Coconut Oil to Increase Proliferation and Cyclooxygenase-2 Expression towards on NIH 3T3 Cell Line in Wound Healing Process
Keywords:Cell proliferation, COX-2, HVCO, NIH 3T3, Percent of the wound closed
AIM: This study aims to determine the effect of hydrolysed virgin coconut oil (HVCO) to increase cell proliferation, COX-2 expression of NIH 3T3.
METHODS: The sample used was Virgin Coconut Oil (VCO). VCO was partially hydrolysed using lipase from Rhizomucor miehei (active on sn-1,3 position) to produce hydrolysed VCO (HVCO) composed of free fatty acids, 2-monoglycerides. Then acid value was determined. The effect of HVCO on proliferation was evaluated using the MTT method. Wound healing assay was established by a cell migration method, and COX-2 expression was determined using RT-PCR.
RESULTS: Acid value is 135.89 Â± 0.12 mg NaOH/g oil and free fatty acids (FFA) is 48.50 Â± 0.06%. The effect of HVCO 62.5 Âµg/mL on cell proliferation after 24h, 48h, and 72h incubation found as viable cells are 109.24 Â± 0.52%; 118.26 Â± 0.91% and 106.59 Â± 0.74%. Percent of wound closed after 24 h and 48 h incubation are 69.94 Â± 0.54% and 100.00 Â± 0.00%, and expression of COX-2 increased from 1 (control) to 1.83 (HVCO).
CONCLUSION: The results suggest that HVCO is effective to increase cells proliferation and hence wound healing process.
Plum Analytics Artifact Widget Block
How to Cite
Copyright (c) 2019 Jansen Silalahi, Yuandani Yuandani , Dian Ika Perbina Br Meliala, Linda Margata, Denny Satria (Author)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.