@article{Hammam_Magdy_Badawy_Al Osili_El Kholy_El LeitHy_2017, title={Expression of MDM2 mRNA, MDM2, P53 and P16 Proteins in Urothelial Lesions in the View of the WHO 4th Edition Guidelines as A Molecular Insight towards Personalized Medicine}, volume={5}, url={https://oamjms.eu/index.php/mjms/article/view/oamjms.2017.100}, DOI={10.3889/oamjms.2017.100}, abstractNote={<p><strong>AIM:</strong> Here we imposed a multimarker molecular panel composed of P53, MDM2 protein & mRNA & P16 with the identification of sensitive and specific cut offs among the Egyptian urothelial carcinomas bilharzial or not emphasize the pathological and molecular classifications, pathways and prognosis as a privilege for adjuvant therapy.</p><p><strong>METHODS:</strong> Three hundred and ten urothelial lesions were pathologically evaluated and grouped as follows: 50 chronic cystitis as benign, 240 urothelial carcinomas and 20 normal bladder tissue as a control. Immunohistochemistry for MDM Protein, P16 & p53 and In Situ Hybridization for MDM2mRNA were done.</p><p><strong>RESULTS:</strong> MDM2mRNA overexpression correlated with low grade low stage non invasive tumors, while P53 > 40% & p16 < 10% cut offs correlated with high grade high stage invasive carcinomas & bilharzial tumors (P=0.000).</p><strong>CONCLUSION:</strong> MDM2mRNA overexpression vs. P53 > 40% & P16 < 10% constitutes a multimarker molecular panel with significant cut offs, proved to distinguish low grade, low stage non invasive urothelial carcinomas (MDM2mRNA overexpression, P53 < 40%, P16 > 10%) from high grade, high stage invasive urothelial carcinomas (with p53 > 40, p16 < 10% & absent MDM2mRNA overexpression). Combined P53 > 40 & p16 < 10%, together with the histopathological features can distinguish in situ urothelial lesions from dysplastic and atypical lesions.}, number={5}, journal={Open Access Macedonian Journal of Medical Sciences}, author={Hammam, Olfat and Magdy, Mona and Badawy, Mohamed and Al Osili, Khalid and El Kholy, Amr and El LeitHy, Tarek}, year={2017}, month={Aug.}, pages={578–586} }