@article{Aliska_Dhuha_Rahmatini_Hamdani_Afriani_Tofrizal_Ali_Pratiwi_Nuranisyah_Mahata_Kurnia_2021, title={Ramipril Increases Adma Concentration in Acute Myocardial Infarction in Rats Induced by Isoproterenol}, volume={9}, url={https://oamjms.eu/index.php/mjms/article/view/6572}, DOI={10.3889/oamjms.2021.6572}, abstractNote={<p>In experimental animals, the induction of isoproterenol which is a synthetic of catecholamine, can cause acute myocardial infarction where the pathophysiology and morphology are the same as myocardial infarction in humans. Isoproterenol induction will increase oxidative stress, which will damage the enzyme dimethylarginine dimethylaminohydrolase (DDAH), thus causing asymmetric dimethylarginine (ADMA) levels to increase in circulation. Increased levels of ADMA will inhibit the activity of the enzyme nitric oxide synthase, which results in decreased nitric oxide resulting in endothelial damage. This study aims to determine the effect of Ramipril on asymmetric dimethylarginine (ADMA) levels in rats (Rattus norvegicus) Wistar strain with acute myocardial infarction.</p> <p>Eighteen male Wistar rats (150-250 g) were randomly allocated into three groups: negative control group, positive control, and treatment group. The treatment group was pretreated with Ramipril at dose 3 mg/kg BW orally for seven days. Acute myocardial infarction was induced in positive control groups and treatment groups by subcutaneous injection of isoproterenol (85 mg/kg BW) for two consecutive days. Twenty-four hours after the last administration, rats from all groups were anesthetized and sacrificed for blood sample collection to evaluate the level of Asymmetric Dimethylarginine with Enzym-linked Immunosorbent Assay (ELISA) method. The result showed that ADMA levels were increased in the treatment group after pretreated with Ramipril. This study concluded that pretreatment with Ramipril increased ADMA concentration in acute myocardial infarction rats induced by isoproterenol.</p>}, number={A}, journal={Open Access Macedonian Journal of Medical Sciences}, author={Aliska, Gestina and Dhuha, Alief and Rahmatini, Rahmatini and Hamdani, Rita and Afriani, Nita and Tofrizal, Tofrizal and Ali, Hirowati and Pratiwi, Nisa and Nuranisyah, Vinta and Mahata, Liganda Endo and Kurnia, Dedy}, year={2021}, month={Aug.}, pages={693–697} }