@article{Mukhametzhanova_Asanova_Adekenova_Medeubayeva_Kishkentayeva_Adekenov_2022, title={Chartolepis intermedia Boiss. and Centaurea ruthenica Lam. – New Medicina Plants Containing Pharmacologically Active Compounds}, volume={10}, url={https://oamjms.eu/index.php/mjms/article/view/7232}, DOI={10.3889/oamjms.2022.7232}, abstractNote={<div> <p class="Pa6"><strong><span lang="EN-GB">BACKGROUND: </span></strong><em><span lang="EN-GB">Chartolepis intermedia </span></em><span lang="EN-GB">Boiss. and <em>Centaurea ruthenica </em>Lam. are new medicinal plants containing pharmacologically active sesquiterpene lactones grossheimin and cynaropicrin. The article discusses the methods for the isolation of grossheimin and cynaropicrin and presents the results of studying the pharmacological activity of the isolated sesquiterpene lactones.</span></p> </div> <div> <p class="Pa6"><strong><span lang="EN-GB">AIM: </span></strong><span lang="EN-GB">The aim of this work was chemical, morphological, and anatomical study of <em>Ch. intermedi</em>a Boiss. and <em>C. ruthenic</em>a Lam., determination of pharmacological activity to substantiate the possibility of using these plants as medicinal raw materials.</span></p> </div> <div> <p class="Pa6"><strong><span lang="EN-GB">METHODS: </span></strong><span lang="EN-GB">The antitumor activity of grossheimin was studied in white outbred rats and mice in 12 strains of transplanted tumors. The antiparasitic activity of grossheimin and cynaropicrin was studied during replication of models of experimental opisthorchiasis on male golden hamsters (<em>Mesocricetus auratus</em>) of the SPF category. Experiments <em>in vivo </em>were carried out on pastoral dogs infested with various types of helminths. The hypolipidemic activity of grossheimin was studied in experimental rats with a single intragastric administration of ethanol, causing acute hyperlipidemia. The antimicrobial activity of cynarpicrin was determined in relation to test strains of Gram-positive bacteria <em>Staphylococcus aureus </em>and <em>Bacillus subtilis </em>and Gram-negative strains of <em>Escherichia coli </em>and <em>Pseudomonas aeruginosa </em>by the method of diffusion in agar.</span></p> </div> <div> <p class="Pa6"><strong><span lang="EN-GB">RESULTS: </span></strong><span lang="EN-GB">Characteristic diagnostic features for <em>Ch. intermedia </em>Boiss. and <em>C. ruthenica </em>Lam. were revealed, which make it possible to diagnose and standardize the medicinal plants under study. Pharmacological studies have shown that grossheimin and cynaropicrin have antitumor, antiparasitic, hypolipidemic, and antimicrobial activity. Grossheimin significantly inhibits the growth of sarcoma 180, Pliss lymphosarcoma, and Ehrlich’s solid tumor. On the model of acute hyperlipidemia caused by the introduction of ethanol, grossheimin had a hypolipidemic effect, reducing the serum levels of triacylglycerides, total cholesterol, and low-density lipoprotein cholesterol. Under <em>in vitro </em>conditions, grossheimin and cynaropicrin exhibited anthelmintic action against <em>Opisthorchis felineus </em>worms, and under <em>in vivo </em>conditions, cynaropicrin was up to 100% effective against tapeworm infections (<em>Taenia </em>sp.).</span></p> </div> <div><strong>CONCLUSION: </strong>It was determined that the quantitative yield of sesquiterpene lactones is provided by extraction of plant raw materials with an ethanol: water (1:1) mixture under the action of ultrasound. Characteristic diagnostic features for <em>C. intermedia </em>Boiss. and <em>C. ruthenica </em>Lam. were revealed, which make it possible to diagnose and standardize the medicinal plants under study.</div>}, number={A}, journal={Open Access Macedonian Journal of Medical Sciences}, author={Mukhametzhanova, Gaisha and Asanova, Gulzina and Adekenova, Gulimzhan S. and Medeubayeva, Balzhan and Kishkentayeva, Anarkul and Adekenov, Sergazy}, year={2022}, month={Jan.}, pages={56–64} }