TY - JOUR AU - Elfakhrany, Amany AU - Abo-Elsoud, Reda Abo Elfath Ahmed AU - Abd El Kareem, Heba Mohamed AU - Samaka, Rehab Monir AU - Elfiky, Safa Reyad PY - 2022/02/05 Y2 - 2024/03/28 TI - Autophagy and Oxidative Balance Mediate the Effect of Carvedilol and Glibenclamide in a Rat Model of Renal Ischemia-Reperfusion Injury JF - Open Access Macedonian Journal of Medical Sciences JA - Open Access Maced J Med Sci VL - 10 IS - A SE - Physiology DO - 10.3889/oamjms.2022.10125 UR - https://oamjms.eu/index.php/mjms/article/view/10125 SP - 1402-1410 AB - <div><p class="Pa6"><strong><span lang="EN-GB">BACKGROUND: </span></strong><span lang="EN-GB">Reactive oxygen species and cytokines are the main players in the development of renal ischemia-reperfusion (I/R) injury.</span></p></div><div><p class="Pa6"><strong><span lang="EN-GB">AIM: </span></strong><span lang="EN-GB">The current study aimed to evaluate the effects of carvedilol and/or glibenclamide and the interaction between autophagy and oxidative stress.</span></p></div><div><p class="Pa6"><strong><span lang="EN-GB">METHODS: </span></strong><span lang="EN-GB">50 male rats were divided into five groups: Control, IR injury (IRI), carvedilol pretreated, glibenclamide pretreated, and combined carvedilol and glibenclamide pretreated. Measurements of renal blood flow (RBF), creatinine clearance, serum blood urea nitrogen (BUN), histopathological, and immunohistochemical evaluation of autophagy marker Becl-1 in the rat kidney were performed. Beclin-1and light chain 3 (LC3) Mrna expression was detected by real time polymerase chain reaction.</span></p></div><div><p class="Pa6"><strong><span lang="EN-GB">RESULTS: </span></strong><span lang="EN-GB">IRI was associated with significant increases in BUN, tumor necrosis factor-alpha, nuclear factor κB, and histo (H) score value of Becl-1. However, there was a significant decrease in RBF, creatinine clearance, and glutathione peroxidase compared to the control group. There was significant increase in Beclin-1 and LC3 mRNA gene expression in carvedilol, glibenclamide, and combined treatment groups as compared to IRI and control groups. Combination of carvedilol and glibenclamide significantly restored IRI changes when compared with the other pretreated groups.</span></p></div><div><strong>CONCLUSION: </strong>This study suggests that carvedilol and glibenclamide are promising reno-protective drugs to reduce renal injury induced by I/R through their antioxidant and autophagy stimulation.</div> ER -