TY - JOUR AU - Munilson, Jacky AU - Edward, Yan AU - Fitra Dwita, Lorensia AU - Ali, Hirowati PY - 2021/10/14 Y2 - 2024/03/28 TI - Expression of Cytokeratin 6 and 16 in Middle Ear Cholesteatoma JF - Open Access Macedonian Journal of Medical Sciences JA - Open Access Maced J Med Sci VL - 9 IS - B SE - Ear, Nose and Throat DO - 10.3889/oamjms.2021.6248 UR - https://oamjms.eu/index.php/mjms/article/view/6248 SP - 1323-1328 AB - <div><p class="Pa6"><strong><span lang="EN-US">BACKGROUND: </span></strong><span lang="EN-US">Cholesteatoma is hyperproliferative because of the response of direct biomechanical trauma, and inflammation processes then lead to temporal bone destruction with some clinical manifestations of complications. The hyperproliferation mechanism occurred because of the activation of intermediate filament protein type I and type II known as cytokeratin (CK).</span></p></div><div><p class="Pa6"><strong><span lang="EN-US">AIM: </span></strong><span lang="EN-US">This study aimed to examine the expression CK 6 and CK 16 in cholesteatoma.</span></p></div><div><p class="Pa6"><strong><span lang="EN-US">METHODS: </span></strong><span lang="EN-US">This is a cross-sectional comparative study. Cholesteatoma specimens obtained from 15 patients who underwent surgery were considered as the case, and 15 normal retro-auricular skins were considered as the control. All samples were examined for expression through immunohistochemistry and scored using the immunoreactivity score. Data were analyzed using SPSS via </span><span lang="EN-US">χ</span><sup><span lang="EN-US">2 </span></sup><span lang="EN-US">test, and the difference was significant (p &lt; 0.05).</span></p></div><div><p class="Pa6"><strong><span lang="EN-US">RESULTS: </span></strong><span lang="EN-US">The expression of CK 6 was high in cholesteatoma (33.3%) and low in retro-auricular skin. The expression of CK 16 was high in all samples of cholesteatoma and mostly high in the retro-auricular skin; both expressions were statistically significant (p &lt; 0.05).</span></p></div><div><strong>CONCLUSION: </strong>The expression of CK 6 and CK 16 in cholesteatoma was higher than in normal retro-auricular skin.</div> ER -