TY - JOUR AU - Aulanni’am, Aulanni’am AU - Raissa, Ricadonna AU - Riawan, Wibi AU - Wuragil, Dyah Kinasih AU - Permata, Fajar Shodiq AU - Beltran, Ma Asuncion Guiang PY - 2022/04/27 Y2 - 2024/03/29 TI - Epidermal Stem Cell in Wound Healing of Gliricidia sepium Leaves from Indonesia and the Philippines in Rats (Rattus norvegicus) JF - Open Access Macedonian Journal of Medical Sciences JA - Open Access Maced J Med Sci VL - 10 IS - A SE - Immunology DO - 10.3889/oamjms.2022.8637 UR - https://oamjms.eu/index.php/mjms/article/view/8637 SP - 1143-1150 AB - <div><p class="Pa6"><strong><span lang="EN-US">AIM: </span></strong><span lang="EN-US">This study intended to investigate the regenerate wound, due to the ointment therapy containing <em>Gliricidia sepium </em>leaves that has potential-induced epidermal stem cells producing. It determined its effect on the expression of transforming growth factor-β1 (TGF-β1), Smad-3, β-catenin, LGR-6.</span></p></div><div><p class="Pa6"><strong><span lang="EN-US">MATERIALS AND METHODS: </span></strong><span lang="EN-US">About 16 Wistar male rats aged approximately 2 months (150–200g) were used and were divided into four treatment groups (T1, positive control; T2, negative control; T3, wounds treated with <em>G. sepium </em>from Indonesia; and T4, wounds treated with <em>G. sepium </em>from the Philippines). The treatment of ointment was applied to the wound for 3 days. The expression of TGF-β1, Smad-3, β-catenin, and LGR-6 was observed by immunohistochemistry staining.</span></p></div><div><p class="Pa6"><strong><span lang="EN-US">RESULTS: </span></strong><em><span lang="EN-US">G. sepium </span></em><span lang="EN-US">leaves significantly (p &lt; 0.05) upregulated the expression of TGF-β1, Smad-3, β-catenin, and LGR-6 in the group treated with Indonesian <em>G. sepium </em>leaves were higher than that in the group treated with <em>G. sepium </em>leaves from the Philippines.</span></p></div><div><strong>CONCLUSIONS: </strong>Both leaves Varian contain flavonoids, saponins, and tannins, which act as producing epidermal stem cell agents to enhance the wound healing process. It can be concluded that both <em>Gl. sepium </em>Varian Indonesia and the Philippines have a potential effect on wound healing.</div> ER -