TY - JOUR AU - Endrinaldi, Endrinaldi AU - Ali, Hirowati AU - Tofrizal, Tofrizal AU - Asterina, Asterina AU - Elmatris, Elmatris AU - Yarni, Sisca Dwi PY - 2022/03/09 Y2 - 2024/03/29 TI - Optimization of the Duration of the Administration of Mesenchymal Stem Cells Wharton’s Jelly to the Level of Matrix Metalloproteinase-1 and Transforming Growth Factor-β in Osteoarthritis Rat Model JF - Open Access Macedonian Journal of Medical Sciences JA - Open Access Maced J Med Sci VL - 10 IS - A SE - Immunology DO - 10.3889/oamjms.2022.8838 UR - https://oamjms.eu/index.php/mjms/article/view/8838 SP - 563-571 AB - <div><p class="Pa6"><strong><span lang="EN-GB">BACKGROUND: </span></strong><span lang="EN-GB">Mesenchymal Stem Cell Wharton’s Jelly (MSC-WJ) is promising candidates for osteoarthritis (OA) therapy since they have chondrogenic potential and the ability to form the extracellular matrix. </span></p></div><div><p class="Pa6"><strong><span lang="EN-GB">AIM: </span></strong><span lang="EN-GB">This study aimed to determine the effect of the time giving MSC-WJ on bioactive markers of osteoarthritis.</span></p></div><div><p class="Pa6"><strong><span lang="EN-GB">METHODS: </span></strong><span lang="EN-GB">The osteoarthritis rat model was treated by intra-articular injection with MSC-WJ and α _MEM as a control. Four and 8 weeks later performed a histological analysis of cartilage and the determination of the levels of Matrix Metalloproteinase-1(MMP-1) and Transforming growth factor β1 (TGF-β1) in serum by ELISA.</span></p></div><div><p class="Pa6"><strong><span lang="EN-GB">RESULTS: </span></strong><span lang="EN-GB">The results showed that administration of MSC-WJ showed improvement in the histological picture of knee joints in experimental animals characterized by an increase in cartilage thickness on the joint surface. The administration of MSC-WJ showed a tendency to decrease MMP-1 serum levels of OA rats treated for 8 weeks, although statistically did not show a significant difference. Whereas, administration of MSC-WJ showed a decrease in serum levels of TGF-β1 OA rat treated for 8 weeks.</span></p></div><div><strong>CONCLUSION: </strong>MSC-WJ can repair damaged knee OA cartilage tissue. The administration of MSC-WJ can reduce serum levels of TGF-β1 OA rats treated for 8 weeks.</div> ER -