TY - JOUR AU - Sukrama, I Dewa Made AU - Pinatih, Komang Januartha Putra AU - Hendrayana, Made Agus AU - Rasyid, Burhannuddin AU - Wedari, Ni Luh Putu Harta PY - 2022/03/30 Y2 - 2024/03/28 TI - Escherichia coli Clonal Variability Based on Genetic Diversity Pattern with Enterobacterial Repetitive Intergenic Consensus-Polymerase Chain Reaction Methods for Traveler’s Diarrhea Cases in Bali JF - Open Access Macedonian Journal of Medical Sciences JA - Open Access Maced J Med Sci VL - 10 IS - A SE - Microbiology DO - 10.3889/oamjms.2022.8990 UR - https://oamjms.eu/index.php/mjms/article/view/8990 SP - 752-760 AB - <div><p class="Pa6"><strong><span lang="EN-GB">BACKGROUND: </span></strong><span lang="EN-GB">Bali is a favorite tourism destination in the world. As a major tourist destination, the incidence of illness that afflicts tourists greatly affects the image of tourism. Diarrhea is a health problem that is most often experienced and is a major obstacle for foreign tourists when traveling, especially to Bali. <em>Escherichia coli (E. coli) </em>bacteria cause diarrhea more often than viruses in some developing countries. Genetic differences can affect the characteristics of <em>E. coli</em>, especially in relation to the medical field.</span></p></div><div><p class="Pa6"><strong><span lang="EN-GB">AIM: </span></strong><span lang="EN-GB">We would like to assess the genetic diversity of the different pathogenic <em>E. coli </em>from various clinical isolates including those from traveler’s diarrhea in Bali, Indonesia.</span></p></div><div><p class="Pa6"><strong><span lang="EN-GB">MATERIALS AND METHODS: </span></strong><span lang="EN-GB">One of the molecular techniques used in this study is to use enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR). The sample in this study was the feces of foreign tourists with traveler’s diarrhea in Bali. This study carried out research procedures in the form of Isolation of <em>E. coli </em>genome DNA from culture, amplification of <em>E. coli </em>16S rRNA encoding genes, sequencing of <em>E. coli </em>16S rRNA encoding genes, phylogenetic tree construction, and then analysis of <em>E. coli </em>genetic diversity with ERIC-PCR sequences.</span></p></div><div><p class="Pa6"><strong><span lang="EN-GB">RESULTS: </span></strong><span lang="EN-GB">The results showed that the ERIC-PCR method was more discriminatory than other methods to analyze the genetic diversity of <em>E. coli </em>from fecal samples of patients with traveler’s diarrhea. It was found that clonal variability based on the genetic similarity of all sample <em>E. coli </em>isolates varied from 0% to 100%.</span></p></div><div><strong>CONCLUSIONS: </strong>This shows that the source of transmission and the strains of <em>E. coli </em>that cause it comes from diverse populations.</div> ER -