Oxidative Role of Aflatoxin B1 on the Liver of Wheat Milling Workers

Amal Saad-Hussein; Mohgah Sh. Abdalla; Wafaa Gh. Shousha; Gehan Moubarz; Aya H. Mohamed

doi:10.3889/oamjms.2014.026

Authors

Amal Saad-Hussein Department of Environmental & Occupational Medicine, National Research Centre, El-Behoos Street, Dokki, Cairo 12311
Mohgah Sh. Abdalla Department of Chemistry, Faculty of Science, Helwan University
Wafaa Gh. Shousha Department of Chemistry, Faculty of Science, Helwan University, Cairo
Gehan Moubarz Department of Environmental & Occupational Medicine, National Research Centre, El-Behoos Street, Dokki, Cairo 12311, Egypt; Department of Chemistry, Faculty of Science and Arts-Khulais, King Abdulaziz University, Saudi Arabia
Aya H. Mohamed Department of Chemistry, Faculty of Science, Helwan University, Cairo

DOI:

https://doi.org/10.3889/oamjms.2014.026

Keywords:

Aflatoxin B1, liver enzymes, P53, oxidative stress, antioxidants.

Abstract

Aim: The study aimed to estimate oxidative role of aflatoxin B1 (AFB1) on the liver in wheat milling workers.

Materials and Methods: Case-control study was conducted to compare between the levels of AFB1/albumin (AFB1/alb), liver enzymes (ALT, AST, GGT, and ALP), P53, MDA, GST, SOD, zinc and vitamin C in 35 wheat milling workers and 40 control subjects.

Results: Statistical analysis revealed that ALT, AST, GGT, ALP, P53, MDA, GST and SOD in workers were significantly elevated compared to their controls. In the milling workers, there were significant correlations between MDA levels and the levels of AST, GGT, and P53, while, P53 was inversely correlated with GST and SOD activities. There were significant correlations between Zn levels and GGT, GST and SOD activities, between vitamin C and GST activities, and vitamin C inversely correlated with MDA.

Conclusion: The present study concluded that the oxidative stress of AFB1 elevated the MDA and the liver enzymes in wheat milling workers. GST has a crucial role in the detoxification of aflatoxin and SOD as a scavenger antioxidant increased in the workers to overcome the oxidative toxic effects of AFB1 on the liver of the workers, and roles of Zn and vitamin C were significant in activation of these processes.

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