Comparison of Deep Partial Thickness Burns Wound Healing with Resveratrol Gel and Paraffin Gauze in Wistar Rats
DOI:
https://doi.org/10.3889/oamjms.2024.10107Keywords:
wound care, deep partial thickness burns, resveratrolAbstract
BACKGROUND: Various modalities of wound care in burn cases are offered, but there is no standard algorithm used. Paraffin gauze dressing, as one of the commonly used dressings, is considered to have many shortcomings. Resveratrol, a stilbenoid derived from plants, is reported to have a role in wound healing process; it has the potential to be one of the modalities for treating burns. This study aims to strengthen studies on the role of resveratrol in wound healing process, especially in burns compared with paraffin gauze.
AIM: This study conducted to prove that resveratrol gel more superior than paraffin gauze in deep partial thickness burns healing process.
METHODS: This research is a laboratory experimental study with a post-test only design, simple randomized, and single blind. A sample of 32 Wistar rats with deep partial thickness burns was divided into four control groups and four treatment groups. Control groups were treated using paraffin gauze. Treatment groups were treated using resveratrol gel. Group 1 of each group was assessed for epithelialization. Angiogenesis, fibroblasts, and collagen deposition was assessed on day 5 in group 2, day 14 in group 3, and day 21 in group 4.
RESULTS: Angiogenesis significantly higher in the treatment group than the control group on day 5 and 14 (p = 0.047 and 0.032) but not significantly different on day 21 (p = 0.107). The number of fibroblasts in the treatment group was significantly higher on day 5, 14, and 21 (p = 0.004; 0.038; and 0.005). Collagen deposition not significantly different on day 5 (p = 0.342) but significantly higher on day 14 and 21 (p = 0.048 and 0.002). The epithelialization process occurred earlier in the treatment group than in the control group (p = 0.001).
CONCLUSION: Topical administration of resveratrol gel accelerates the epithelialization process, which increases the number of angiogenesis, fibroblasts, and collagen deposition in deep partial thickness burns compared to paraffin gauze.
Downloads
Metrics
Plum Analytics Artifact Widget Block
References
Shevelev AB, Porta NL, Isakova EP, Martens S, Biryukova YK, Belous AS, et al. In vivo antimicrobial and wound-healing activity of resveratrol, dihydroquercetin, and dihydromyricetin against Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans. Pathogens. 2020;9(4):296. https://doi.org/10.3390/pathogens9040296 PMid:32316572 DOI: https://doi.org/10.3390/pathogens9040296
Hecker A, Schellnegger M, Hofmann E, Luze H, Nichwitz SP, Kamolz LP, et al. The impact of resveratrol on skin wound healing, scarring, and aging. Int Wound J. 2021;19(1):9-28. https://doi.org/10.1111/iwj.13601 PMid:33949795 DOI: https://doi.org/10.1111/iwj.13601
Yaman I, Derici H, Kara C, Kamer E, Diniz G, Ortac R, et al. Effects of resveratrol on incisional wound healing in rats. Surg Today. 2013;43(12):1433-8. https://doi.org/10.1007/s00595-012-0455-7 PMid:23242670 DOI: https://doi.org/10.1007/s00595-012-0455-7
Lin LX, Wang P, Wang YT, Huang Y, Jiang L, Wang XM. Aloe vera and Vitis vinifera improve wound healing in an in vivo rat burn wound model. Mol Med Rep. 2016;13(2):1070-6. https://doi.org/10.3892/mmr.2015.4681 PMid:26677006 DOI: https://doi.org/10.3892/mmr.2015.4681
Fan D, Liu C, Guo Z, Huang K, Peng M, Li N, et al. Resveratrol promotes angiogenesis in a Foxo1-dependent manner in hind limb ischemia in mice. Molecules. 2021;26(24):7528. https://doi.org/10.3390/molecules26247528 PMid:34946610 DOI: https://doi.org/10.3390/molecules26247528
Afshar M, Hassanzadeh-Taheri MM, Zardast M, Moghaddam A. The angiogenetic effect of resveratrol on dermal wound healing in Balb/C mice. Mod Care J. 2017;14(4):e66118. https://doi.org/10.5812/modernc.66118 DOI: https://doi.org/10.5812/modernc.66118
Girbovan C, Kent P, Merali Z, Plamondon H. Dose-related effects of chronic resveratrol administration on neurogenesis, angiogenesis, and corticosterone secretion are associated with improved spatial memory retention following global cerebral ischemia. Nutr Neurosci. 2016;19(8):352-68. https://doi.org/10.1179/1476830515Y.0000000020 PMid:25866012 DOI: https://doi.org/10.1179/1476830515Y.0000000020
Robich MP, Osipov RM, Nezafat R, Feng J, Clements RT, Bianchi C, et al. Resveratrol improves myocardial perfausion in a swine model of hypercholesterolemia and chronic myocardial ischemia. Circulation. 2010;122(11Suppl):S142-9. https://doi.org/10.1161/CIRCULATIONAHA.109.920132 PMid:20837905 DOI: https://doi.org/10.1161/CIRCULATIONAHA.109.920132
Wong JC, Fiscus RR. Resveratrol at anti-angiogenesis/ anticancer concentrations suppresses protein kinase G signaling and decreases IAPs expression in HUVECs. Anticancer Res. 2015;35(1):273-81 PMid:25550561
Kaleci B, Koyuturk M. Efficacy of resveratrol in the wound healing process by reducing oxidative stress and promoting fibroblast cell proliferation and migration. Dermatol Ther. 2020;33(6):e14357. https://doi.org/10.1111/dth.14357 PMid:32996685 DOI: https://doi.org/10.1111/dth.14357
Çelen Ç, Keçeciler C, Yapar EA, Gökçe EH, Nalbantsoy A. Evaluation of resveratrol organogels prepared by micro-irradiation: Fibroblast proliferation through in vitro wound healing. Turk J Biochem. 2018;43(4):385-92. https://doi.org/doi:10.1515/tjb-2016-0283 DOI: https://doi.org/10.1515/tjb-2016-0283
Bosutti A, Degens H. The impact of resveratrol and hydrogen peroxide on muscle cell plasticity shows a dose-dependent interaction. Sci Rep. 2015;5:8093. https://doi.org/10.1038/srep08093 PMid:25627702 DOI: https://doi.org/10.1038/srep08093
Ikeda K, Torigoe T, Matsumoto Y, Fujita T, Sato N, Yotsuyanagi T. Resveratrol inhibits fibrogenesis and induces apoptosis in keloid fibroblasts. Wound Repair Regen. 2013;21(4):616-23. https://doi.org/10.1111/wrr.12062 PMid:23815229 DOI: https://doi.org/10.1111/wrr.12062
Zeng G, Zhong F, Li J, Luo S, Zhang P. Resveratrol-mediated reduction of collagen by inhibiting proliferation and producing apoptosis in human hypertrophic scar fibroblasts. Biosci Biotechnol Biochem. 2013;77(12):2389-96. https://doi.org/10.1271/bbb.130502 PMid:24317052. DOI: https://doi.org/10.1271/bbb.130502
Wang P, Huang S, Hu Z, Yang W, Lan Y, Zhu J, et al. In situ formed anti-inflammatory hydrogel loading plasmid DNA encoding VEGF for burn wound healing. Acta Biomater. 2019;100:191-201. https://doi.org/10.1016/j.actbio.2019.10.004 PMid:31586729 DOI: https://doi.org/10.1016/j.actbio.2019.10.004
Prakoeswa CR, Rindiastuti Y, Wirohadidjojo YW, Komaratih E, Nurwasis EK, Dinaryati A, et al. Resveratrol promotes secretion of wound healing related growth factors of mesenchymal stem cells originated from adult and fetal tissues. Artif Cells Nanomed Biotechnol. 2020;48(1):1159-66. https://doi.org/10.1080/216914 01.2020.1817057 PMid:32902361 DOI: https://doi.org/10.1080/21691401.2020.1817057
Downloads
Published
How to Cite
License
Copyright (c) 2024 Paulina Magdalena, I. Made Suka Adnyana, I. Gusti Putu Hendra Sanjaya, Agus Roy Rusly Hariantana Hamid, Ni Wayan Winarti (Author)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
http://creativecommons.org/licenses/by-nc/4.0
