Inhibitory Activity of α-Glucosidase by the Extract and Fraction of Marine Sponge-Derived Fungus Penicillium citrinum Xt6
Keywords:Alpha-glucosidase, Enzyme, Fungus, Marine sponge, Penicillium citrinum
BACKGROUND: Diabetes mellitus is a metabolic condition characterized by high blood glucose levels due to insufficient insulin secretion or activity. Diabetes treatment may include inhibiting carbohydrate breakdown enzymes like α-glucosidase. Chemical compounds of the marine-derived fungus have the potential to inhibit α-glucosidase and, thus, could be used in therapy. Marine sponge-derived fungus lives in a colony on the tissues of living things. In the marine sponge Xestospongia testudinaria DD-01, there is a colony of the fungus Penicillium citrinum Xt6. P. citrinum Xt6 has been reported to reduce blood glucose levels in alloxan-induced diabetic mice.
AIM: This study aimed to investigate the inhibitory activity of α-glucosidase by the extracts and fractions of marine-derived fungus P. citrinum Xt6.
MATERIALS AND METHODS: The study was carried out in vitro using p-nitrophenyl-α-D-glucopyranoside (PNPG) substrate and α-glucosidase enzyme from Saccharomyces cerevisiae. ELISA was used to measuring the enzyme’s inhibition activity at the wavelength of 405 nm. Acarbose was used as the standard drug, which inhibits the activity of α-glucosidase.
RESULTS: Inhibitory concentration (IC50) value of ethyl acetate extract was 37.39 μg/mL, methanol fraction was 60.01 μg/mL, n-hexane fraction was 75.45 μg/mL, and acarbose was 124.39 g/mL.CONCLUSION: It can be concluded that the extract and fraction of marine-derived fungus P. citrinum inhibit α-glucosidase activity. P. citrinum could be developed into an antidiabetic agent.
Plum Analytics Artifact Widget Block
American Diabetes Association (ADA). Introduction: Standards of medical care in diabetes 2019. Diabetes Care. 2019;43(Suppl 1): S1-2. https://doi.org/10.2337/dc20-sint PMid:30559224 DOI: https://doi.org/10.2337/dc20-Sint
International Diabetes Federation IDF. IDF Diabetes Atlas. Ninth ed. Brussels, Belgium: International Diabetes Federation IDF; 2019. p. 1-164.
Elya B, Handayani R, Sauriasari R, Azizahwati, Hasyyati US, Permana IT, et al. Antidiabetic activity and phytochemical screening of extracts from Indonesian plants by inhibition of alpha-amylase, alpha-glucosidase, and dipeptidyl peptidase IV. Pak J Biol Sci. 2015;18(6):273-8. https://doi.org/10.3923/pjbs.2015.279.284 DOI: https://doi.org/10.3923/pjbs.2015.279.284
Amiri A, Azemi ME, Khodayar MJ, Namjoyan F. In vitro α-amylase and α-glucosidases inhibitory effects of some plants extracts. Int J Pharmacogn Phytochem Res. 2015; 7(2): 315-318. https://doi.org/10.4103/0973-1296.166018 DOI: https://doi.org/10.4103/0973-1296.166018
Kumar S, Narwal S, Kumar V, Prakash O. α-glucosidase inhibitors from plants: A natural approach to treat diabetes. Pharmacogn Rev. 2011;5(9):19-29. https://doi.org/10.4103/0973-7847.79096 PMid:22096315 DOI: https://doi.org/10.4103/0973-7847.79096
Wang H, Fu Z, Han C. The potential applications of marine bioactives against diabetes and obesity. Am J Mar Sci. 2014;2(1):1-8. https://doi.org/10.1269/marine-2-1-1
Meenupriya J, Thangaraj M. Bioprospecting of potent fungal strains from marine sponge Hyatella cribriformis from gulf of Mannar Coast. Biotechnol Healthc Sci. 2012;72-5.
Zhou X, Xu T, Yang XW, Huang R, Yang B, Tang L, et al. Chemical and biological aspects of marine sponges of the genus Xestospongia. Chem Biodivers. 2010;7(9):2201-27. https://doi.org/10.1002/cbdv.201000024 PMid:20860025 DOI: https://doi.org/10.1002/cbdv.201000024
Bakhtra DD, Suryani R, Yuni GR, Handayani D. Antimicrobial and cytotoxic activities screening of symbiotic fungi extract isolated from marine sponge Xestospongia testudinaria DD-01. J Chem Pharm Sci. 2019;12(02):30-4. https://doi.org/10.30558/jchps.20191202001 DOI: https://doi.org/10.30558/jchps.20191202001
Fauziah F, Oktavia S, Bakhtra DD, Handayani D, Ali H, Ilmiawati C, et al. Effect of ethyl acetate extract of Penicillium citrinum from Xestopongia testudinaria on blood glucose level, insulin concentration, and homeostatic model assessment of insulin resistance (HOMA-IR). Curr Trends Biotechnol Pharm. 2020;14(2):31-2.
Ali S, Khan AL, Ali L, Rizvi TS, Khan SA, Hussain J, et al. Enzyme inhibitory metabolites from endophytic Penicillium citrinum isolated from Boswellia sacra. Arch Microbiol. 2017;199(5):691-700. https://doi.org/10.1007/s00203-017-1348-3 DOI: https://doi.org/10.1007/s00203-017-1348-3
Habtemariam S, Varghese G. The Antidiabetic therapeutic potential of dietary polyphenols. Curr Pharm Biotechnol. 2014;15(4):391-400. https://doi.org/10.2174/1389201015666140617104643 PMid:24938887 DOI: https://doi.org/10.2174/1389201015666140617104643
Ajebli M, Khan H, Eddouks M. Natural alkaloids and diabetes mellitus: A review. Endocrine, Metab Immune Disord: Drug Targets. 2020;21(1):111-30. https://doi.org/10.2174/1871530320666200821124817 PMid:32955004 DOI: https://doi.org/10.2174/1871530320666200821124817
Kjer J, Debbab A, Aly AH, Proksch P. Methods for isolation of marine-derived endophytic fungi and their bioactive secondary products. Nat Protoc. 2010;5(3):479-90. https://doi.org/10.1038/nprot.2009.233 PMid:20203665 DOI: https://doi.org/10.1038/nprot.2009.233
Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical screening and extraction: A Review. Int Pharm Sci. 2011;1(1):98-106.
Handayani D, Ananda N, Artasasta MA, Ruslan R, Fadriyanti O, Tallei TE. Antimicrobial activity screening of endophytic fungi extracts isolated from brown algae Padina sp. J Appl Pharm Sci. 2019;9(3):9-13. https://doi.org/10.7324/JAPS.2019.90302 DOI: https://doi.org/10.7324/JAPS.2019.90302
Zahratunnisa N, Elya B, Noviani A. Inhibition of alpha-glucosidase and antioxidant test of stem bark extracts of Garcinia fruticosa Lauterb. Pharmacogn J. 2017;9(2):273-5. https://doi.org/10.5530/pj.2017.2.46 DOI: https://doi.org/10.5530/pj.2017.2.46
Vasanthabharathi V, Jayalakshmi S. Bioactive potential of symbiotic bacteria and fungi from marine sponges. Afr J Biotechnol. 2012;11(29):7500-11. https://doi.org/10.5897/AJB11.1378 DOI: https://doi.org/10.5897/AJB11.1378
Aminah I, Putra AE, Arbain D, Handayani D. Screening of cytotoxic activities toward WiDr and Vero cell lines of ethyl acetate extracts of fungi-derived from the marine sponge Acanthostrongylophora ingens. J Appl Pharm Sci. 2019;9(1):1-5. https://doi.org/10.7324/JAPS.2019.90101 DOI: https://doi.org/10.7324/JAPS.2019.90101
Blockley A, Elliott DR, Roberts AP, Sweet M. Symbiotic microbes from marine invertebrates: Driving a new era of natural product drug discovery. Diversity. 2017;9(4):1-13. https://doi.org/10.3390/d9040049 DOI: https://doi.org/10.3390/d9040049
Osonoi T. Alpha-glucosidase inhibitor. Nihon Rinsho. 2015;73(3):390-4.
Shi ZL, Liu YD, Yuan YY, Song D, Qi MF, Yang XJ, et al. In vitro and in vivo effects of norathyriol and mangiferin on α-glucosidase. Biochem Res Int. 2017;2017:1206015. https://doi.org/10.1155/2017/1206015 PMid:28168055 DOI: https://doi.org/10.1155/2017/1206015
Mugaranja KP, Kulal A. Alpha-glucosidase inhibition activity of phenolic fraction from Simarouba glauca: An in-vitro, in-silico and kinetic study. Heliyon. 2020;6(7):e04392. https://doi.org/10.1016/j.heliyon.2020.e04392 DOI: https://doi.org/10.1016/j.heliyon.2020.e04392
Aleixandre A, Gil JV, Sineiro J, Rosell CM. Understanding phenolic acids inhibition of α-amylase and α-glucosidase and influence of reaction conditions. Food Chem. 2022;372:131231. https://doi.org/10.1016/j.foodchem.2021.131231 PMid:34624776 DOI: https://doi.org/10.1016/j.foodchem.2021.131231
Kalita D, Holm DG, LaBarbera DV, Petrash JM, Jayanty SS. Inhibition of α-glucosidase, α-amylase, and aldose reductase by potato polyphenolic compounds. PLoS One. 2018;13(1):e0191025. https://doi.org/10.1371/jurnal.pone.0191025 PMid:29370193 DOI: https://doi.org/10.1371/journal.pone.0191025
DeCarvalho LP, Oliveira SE, Aparecida CP, Honorata HL, Ikegaki M. Importance and implications of the production of phenolic secondary metabolites by endophytic fungi: A mini-review. Mini Rev Med Chem. 2016,16(4):259-71. https://doi.org/10.2714/1389557515666151016123923 PMid:26471971 DOI: https://doi.org/10.2174/1389557515666151016123923
How to Cite
Copyright (c) 2022 Fitra Fauziah, Hirowati Ali, Cimi Ilmiawati, Dwi Bakhtra, Zilfia Agustin, Dian Handayani (Author)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.