Evaluation of the Antibiotic Resistance Rate of Helicobacter pylori in Peptic Ulcer Patients in Tien Giang Central General Hospital, Tien Giang Province, Vietnam

Le Tran Thi Nhu; Van Lam Nguyen; Van De Tran; Ngoc Anh Tran; Vu Trung Nguyen

doi:10.3889/oamjms.2022.10216

Authors

Le Tran Thi Nhu Department of Microbiology, Can Tho University of Medicine and Pharmacy, Can Tho, Vietnam; Department of Microbiology, Hanoi Medical University, Hanoi, Vietnam
Van Lam Nguyen Department of Anatomy, Can Tho University of Medicine and Pharmacy, Can Tho, Vietnam
Van De Tran Department of Drug Administration, Can Tho University of Medicine and Pharmacy, Can Tho, Vietnam
Ngoc Anh Tran Department of General Internal Medicine, Hanoi Medical University, Hanoi, Vietnam; Department of Internal Medicine, Hanoi Medical University Hospital, Hanoi, Vietnam
Vu Trung Nguyen Department of Microbiology, Can Tho University of Medicine and Pharmacy, Can Tho, Vietnam; Administration of Science, Technology and Training, Ministry of Health, Hanoi, Vietnam

DOI:

https://doi.org/10.3889/oamjms.2022.10216

Keywords:

Helicobacter pylori, Peptic ulcer, Antibiotic resistance

Abstract

BACKGROUND: Peptic ulcer (PU), trauma on the lining of the stomach and/or small intestine, is among the top five reasons for hospitalization in Tien Giang, a province in the South of Vietnam. Since Helicobacter pylori (HP) is one of the main causes of PU, its features, especially the antibiotic-resistant status, have critical significance in PU treatment.

AIM: This study evaluates the HP infection prevalence, HP antibiotic resistance rate, and its associations with the patients’ sociodemographic characteristics.

MATERIALS AND METHODS: A cross-sectional study was conducted on PU patients in Tien Giang province, Vietnam, from June 2020 to June 2021. The volunteers were tested for HP infection and antibiotic resistance using three methods, where appropriate, including Gram staining, CLO (urease) test, and bacterial culture method.

RESULTS: Among 368 samples, 31.5% had infected with antibiotic-resistant HP. The resistance rates to five antibiotics commonly used in HP treatment, including metronidazole, clarithromycin, tetracycline, levofloxacin, and amoxicillin, were 96.6%, 94.8%, 70.7%, 61.2%, and 53.4%, respectively. The rates of tetracycline and clarithromycin resistance were related to alcohol consumption (t-test, p < 0.05). The HP treatment history was significantly associated with the levofloxacin resistance (t-test, p < 0.05).

CONCLUSIONS: The emergence of antibiotic-resistant HP is a major public health concern in Tien Giang, Vietnam. This issue should be tackled at the national level to avoid the further spread of these multi-drug resistant HP strains.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Plum Analytics Artifact Widget Block

References

Peek RM Jr., Blaser MJ. Helicobacter pylori and gastrointestinal tract adenocarcinomas. Nat Rev Cancer. 2002;2:28-37. http://doi.org/10.1038/nrc703 PMid:11902583 DOI: https://doi.org/10.1038/nrc703

Suerbaum S, Michetti P. Helicobacter pylori infection. N Engl J Med. 2002;347(15):1175-86. https://doi.org/10.1056/NEJMRA020542 DOI: https://doi.org/10.1056/NEJMra020542

Peek RM Jr., Crabtree JE. Helicobacter infection and gastric neoplasia. J Pathol. 2006;208(2):233-48. http://doi.org/10.1002/path.1868 PMid:16362989 DOI: https://doi.org/10.1002/path.1868

Binh TT, Shiota S, Nguyen LT, Ho DD, Hoang HH, Ta L, et al. The incidence of primary antibiotic resistance of Helicobacter pylori in Vietnam. J Clin Gastroenterol. 2013;47(3):233-8. http://doi.org/10.1097/MCG.0b013e3182676e2b PMid:23090037 DOI: https://doi.org/10.1097/MCG.0b013e3182676e2b

Liu DS, Wang YH, Zhu ZH, Zhang SH, Zhu X, Wan JH, et al. Characteristics of Helicobacter pylori antibiotic resistance: Data from four different populations. Antimicrob Resist Infect Control. 2019;8:192. http://doi.org/10.1186/s13756-019-0632-1 PMid:31798838 DOI: https://doi.org/10.1186/s13756-019-0632-1

Hoang TT, Bengtsson C, Phung DC, Sörberg M, Granström M. Seroprevalence of Helicobacter pylori infection in urban and rural Vietnam. Clin Diagn Lab Immunol. 2005;12(1):81-5. http://doi.org/10.1128/CDLI.12.1.81-85.2005 PMid:15642989 DOI: https://doi.org/10.1128/CDLI.12.1.81-85.2005

Quach DT, Luu MN, Hiyama T, To TH, Bui QN, Tran TA, et al. Early diagnosis of Helicobacter pylori infection in vietnamese patients with acute peptic ulcer bleeding: A prospective study. Gastroenterol Res Pract. 2017;2017:3845067. http://doi.org/10.1155/2017/3845067 PMid:28133477 DOI: https://doi.org/10.1155/2017/3845067

Qasim A, O’Morain CA. Review article: Treatment of Helicobacter pylori infection and factors influencing eradication. Aliment Pharmacol Ther. 2002;16 Suppl 1:24-30. http://doi.org/10.1046/j.1365-2036.2002.0160s1024.x PMid:11849124 DOI: https://doi.org/10.1046/j.1365-2036.2002.0160s1024.x

Suzuki T, Matsuo K, Ito H, Sawaki A, Hirose K, Wakai K, et al. Smoking increases the treatment failure for Helicobacter pylori eradication. Am J Med. 2006;119:217-24. http://doi.org/10.1016/j.amjmed.2005.10.003 PMid:16490464 DOI: https://doi.org/10.1016/j.amjmed.2005.10.003

Jenks PJ. Causes of failure of eradication of Helicobacter pylori. BMJ. 2002;325(7354):3-4. http://doi.org/10.1136/bmj.325.7354.3 PMid:12098709 DOI: https://doi.org/10.1136/bmj.325.7354.3

Di KN, Tay ST, Ponnampalavanar SS, Pham DT, Wong LP. Socio-demographic factors associated with antibiotics and antibiotic resistance knowledge and practices in Vietnam: A cross-sectional survey. Antibiotics (Basel). 2022;11(4):471. http://doi.org/10.3390/antibiotics11040471 PMid:35453222 DOI: https://doi.org/10.3390/antibiotics11040471

Di KN, Pham DT, Tee TS, Binh QA, Nguyen TC. Antibiotic usage and resistance in animal production in Vietnam: A review of existing literature. Trop Anim Health Prod. 2021;53(3):340. http://doi.org/10.1007/s11250-021-02780-6 PMid:34089130 DOI: https://doi.org/10.1007/s11250-021-02780-6

Hooi JK, Lai WY, Ng WK, Suen MM, Underwood FE, Tanyingoh D, et al. Global prevalence of Helicobacter pylori infection: Systematic review and meta-analysis. Gastroenterology. 2017;153(2):420-9. http://doi.org/10.1053/j.gastro.2017.04.022 PMid:28456631 DOI: https://doi.org/10.1053/j.gastro.2017.04.022

Le LT, Nguyen TA, Nguyen NA, Nguyen YT, Nguyen HT, Nguyen LT, et al. Antibiotic resistance of Helicobacter pylori in children with gastritis and peptic ulcers in Mekong Delta, Vietnam. Healthcare (Basel). 2022;10(6):1121. http://doi.org/10.3390/healthcare10061121 PMid:35742177 DOI: https://doi.org/10.3390/healthcare10061121

An B, Moon BS, Kim H, Lim HC, Lee YC, Lee G, et al. Antibiotic resistance in Helicobacter pylori strains and its effect on H. pylori eradication rates in a single center in Korea. Ann Lab Med. 2013;33(6):415-9. http://doi.org/10.3343/alm.2013.33.6.415 PMid:24205490 DOI: https://doi.org/10.3343/alm.2013.33.6.415

Mégraud F, Lehours P. Helicobacter pylori detection and antimicrobial susceptibility testing. Clin Microbiol Rev. 2007;20(2):280-322. http://doi.org/10.1128/CMR.00033-06 PMid:17428887 DOI: https://doi.org/10.1128/CMR.00033-06

Graham DY, Adam E, Reddy GT, Agarwal JP, Agarwal R, Evans DJ Jr., et al. Seroepidemiology of Helicobacter pylori infection in India. Comparison of developing and developed countries. Dig Dis Sci. 1991;36(8):1084-8. http://doi.org/10.1007/BF01297451 PMid:1864201 DOI: https://doi.org/10.1007/BF01297451

Asaka M, Kimura T, Kudo M, Takeda H, Mitani S, Miyazaki T, et al. Relationship of Helicobacter pylori to serum pepsinogens in an asymptomatic Japanese population. Gastroenterology. 1992;102(3):760-6. http://doi.org/10.1016/0016-5085(92)90156-s PMid:1537513 DOI: https://doi.org/10.1016/0016-5085(92)90156-S

Graham DY, Malaty HM, Evans DG, Evans DJ Jr., Klein PD, Adam E. Epidemiology of Helicobacter pylori in an asymptomatic population in the United States. Effect of age, race, and socioeconomic status. Gastroenterology. 1991;100(6):1495-501. http://doi.org/10.1016/0016-5085(91)90644-z PMid:2019355 DOI: https://doi.org/10.1016/0016-5085(91)90644-Z

Fock KM, Katelaris P, Sugano K, Ang TL, Hunt R, Talley NJ, et al. Second Asia-Pacific Consensus Guidelines for Helicobacter pylori infection. J Gastroenterol Hepatol. 2009;24(10):1587-600. http://doi.org/10.1111/j.1440-1746.2009.05982.x PMid:19788600 DOI: https://doi.org/10.1111/j.1440-1746.2009.05982.x

Gisbert JP. Second-line rescue therapy of Helicobacter pylori infection. Therap Adv Gastroenterol. 2009;2(6):331-56. http://doi.org/10.1177/1756283X09347109 PMid:21180581 DOI: https://doi.org/10.1177/1756283X09347109

Gisbert JP, Morena F. Systematic review and meta-analysis: Levofloxacin-based rescue regimens after Helicobacter pylori treatment failure. Aliment Pharmacol Ther. 2006;23(1):35-44. http://doi.org/10.1111/j.1365-2036.2006.02737.x PMid:16393278 DOI: https://doi.org/10.1111/j.1365-2036.2006.02737.x

Perna F, Zullo A, Ricci C, Hassan C, Morini S, Vaira D. Levofloxacin-based triple therapy for Helicobacter pylori re-treatment: Role of bacterial resistance. Dig Liver Dis. 2007;39(11):1001-5. http://doi.org/10.1016/j.dld.2007.06.016 PMid:17889627 DOI: https://doi.org/10.1016/j.dld.2007.06.016

Matsumoto Y, Miki I, Aoyama N, Shirasaka D, Watanabe Y, Morita Y, et al. Levofloxacin- versus metronidazole-based rescue therapy for H. pylori infection in Japan. Dig Liver Dis. 2005;37(11):821-5. http://doi.org/10.1016/j.dld.2005.06.002 PMid:16040284 DOI: https://doi.org/10.1016/j.dld.2005.06.002

Dore MP, Osato MS, Realdi G, Mura I, Graham DY, Sepulveda AR. Amoxycillin tolerance in Helicobacter pylori. J Antimicrob Chemother. 1999;43(1):47-54. http://doi.org/10.1093/jac/43.1.47 PMid:10381100 DOI: https://doi.org/10.1093/jac/43.1.47

Okamoto T, Yoshiyama H, Nakazawa T, Park ID, Chang MW, Yanai H, et al. A change in PBP1 is involved in amoxicillin resistance of clinical isolates of Helicobacter pylori. J Antimicrob Chemother. 2002;50(6):849-56. http://doi.org/10.1093/jac/dkf140 PMid:12461003 DOI: https://doi.org/10.1093/jac/dkf140

Du P, Zhang C, Wang A, Ma Z, Shen S, Li X. Association of alcohol drinking and Helicobacter pylori infection: A meta-analysis. J Clin Gastroenterol. 2021:1638. online ahead of print http://doi.org/10.1097/MCG.0000000000001638 PMid:34907920 DOI: https://doi.org/10.1097/MCG.0000000000001638