Potential Use of Some Indonesian Plants to Inhibits Angiotensin-converting Enzyme In Vitro

Ni Made Dwi Sandhiutami; Rika Sari Dewi; Fathya Rahma; Ferry Yang

doi:10.3889/oamjms.2022.10251

Authors

Ni Made Dwi Sandhiutami Department of Pharmacology, Faculty of Pharmacy, Pancasila University, Jakarta, Indonesia https://orcid.org/0000-0003-3449-1998
Rika Sari Dewi Department of Pharmacology, Faculty of Pharmacy, Pancasila University, Jakarta, Indonesia
Fathya Rahma Department of Pharmacology, Faculty of Pharmacy, Pancasila University, Jakarta, Indonesia
Ferry Yang Department of Pharmacology, Faculty of Pharmacy, Pancasila University, Jakarta, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2022.10251

Keywords:

Indonesian plants, ACE inhibitory activity, In vitro, IC50

Abstract

BACKGROUND: Some Indonesian plants, such as Vaccinium varingiaefolium Miq., Plectranthus scutellarioides (L.) R.Br., Syzygium myrtifolium Walp., and Eclipta prostrata (L.) L., are rich of flavonoid and anthocyanin. Flavonoid, flavan-3-ol, quercetin, anthocyanin, and tannin compounds can reduce systemic vascular resistance because they cause vasodilation and are thought to be able to influence the function of angiotensin-converting enzyme (ACE) and inhibit ACE activity, which plays an important role in the process of hypertension.

AIM: This study aims to determine the potential of some Indonesian plants to inhibit ACE activity.

METHODS: Testing of ACE inhibitory activity is carried out by the hippuric acid compounds formed as a result of the reaction between the substrate and the enzyme, then measured spectrophotometrically. The inhibitory and IC50 values of each test sample were compared with the positive control of Captopril.

RESULTS: The four plant extracts contained secondary metabolites, such as flavonoids, tannins, saponins, quinones, steroids, triterpenoids, and essential oils. Ethanol extract of V. varingiaefolium Miq., P. scutellarioides (L.) R.Br., S. myrtifolium Walp., and E. prostrata (L.) L. each had an IC₅₀ value of ACE inhibition activity of 131.4 ppm, 206. 7 ppm, 151.2 ppm, and 196.0 ppm. The IC₅₀ value of the Captopril with inhibition of ACE activity is 11.1 ppm.

CONCLUSION: This study shows that some Indonesian plants have the activity to inhibit the ACE and potential antihypertensive drug candidates with ACE inhibitory activity.

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