Potential Use of Some Indonesian Plants to Inhibits Angiotensin-converting Enzyme In Vitro

Authors

  • Ni Made Dwi Sandhiutami Department of Pharmacology, Faculty of Pharmacy, Pancasila University, Jakarta, Indonesia https://orcid.org/0000-0003-3449-1998
  • Rika Sari Dewi Department of Pharmacology, Faculty of Pharmacy, Pancasila University, Jakarta, Indonesia
  • Fathya Rahma Department of Pharmacology, Faculty of Pharmacy, Pancasila University, Jakarta, Indonesia
  • Ferry Yang Department of Pharmacology, Faculty of Pharmacy, Pancasila University, Jakarta, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2022.10251

Keywords:

Indonesian plants, ACE inhibitory activity, In vitro, IC50

Abstract

BACKGROUND: Some Indonesian plants, such as Vaccinium varingiaefolium Miq., Plectranthus scutellarioides (L.) R.Br., Syzygium myrtifolium Walp., and Eclipta prostrata (L.) L., are rich of flavonoid and anthocyanin. Flavonoid, flavan-3-ol, quercetin, anthocyanin, and tannin compounds can reduce systemic vascular resistance because they cause vasodilation and are thought to be able to influence the function of angiotensin-converting enzyme (ACE) and inhibit ACE activity, which plays an important role in the process of hypertension.

AIM: This study aims to determine the potential of some Indonesian plants to inhibit ACE activity.

METHODS: Testing of ACE inhibitory activity is carried out by the hippuric acid compounds formed as a result of the reaction between the substrate and the enzyme, then measured spectrophotometrically. The inhibitory and IC50 values of each test sample were compared with the positive control of Captopril.

RESULTS: The four plant extracts contained secondary metabolites, such as flavonoids, tannins, saponins, quinones, steroids, triterpenoids, and essential oils. Ethanol extract of V. varingiaefolium Miq., P. scutellarioides (L.) R.Br., S. myrtifolium Walp., and E. prostrata (L.) L. each had an IC50 value of ACE inhibition activity of 131.4 ppm, 206. 7 ppm, 151.2 ppm, and 196.0 ppm. The IC50 value of the Captopril with inhibition of ACE activity is 11.1 ppm.

CONCLUSION: This study shows that some Indonesian plants have the activity to inhibit the ACE and potential antihypertensive drug candidates with ACE inhibitory activity.

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References

Saputri FC, Mun’im A, Lukmanto D, Aisyah SN, Rinandy JS. Inhibition of angiotensin converting enzyme (ACE) activity by some Indonesia edible plants. Int J Pharm Sci Res. 2015;6(3):1054-9. https://doi.org/10.13040/IJPSR.0975-8232.6(3).1054-59 DOI: https://doi.org/10.13040/IJPSR.0975-8232.6(3).1054-59

Borghi C, Rossi F, SIIA Task Force, SIF Task Force. Role of the renin-angiotensin-aldosterone system and its pharmacological inhibitors in cardiovascular diseases: Complex and critical issues. High Blood Press Cardiovasc Prev. 2015;22(4):429-44. https://doi.org/10.1007/s40292-015-0120-5 PMid:26403596 DOI: https://doi.org/10.1007/s40292-015-0120-5

Sharifi N, Souri E, Ziai SA, Amin G, Amanlou M. Discovery of new angiotensin converting enzyme (ACE) inhibitors from medicinal plants to treat hypertension using an in vitro assay. Daru. 2013;21(1):74. https://doi.org/10.1186/2008-2231-21-74 PMid:24359711 DOI: https://doi.org/10.1186/2008-2231-21-74

Lange-Jacobs D, Shaikh-Kader A, Thomas B, Nyakudya TT. An overview of the potential use of ethno-medicinal plants targeting the renin--angiotensin system in the treatment of hypertension. Molecules. 2020;25(9):2114. https://doi.org/10.3390/molecules25092114 PMid:32366012 DOI: https://doi.org/10.3390/molecules25092114

Muñoz-Durango N, Fuentes CA, Castillo AE, González- Gómez LM, Vecchiola A, Fardella CE, et al. Role of the renin-angiotensin-aldosterone system beyond blood pressure regulation: Molecular and cellular mechanisms involved in end-organ damage during arterial hypertension. Int J Mol Sci. 2016;17(7):797. https://doi.org/10.3390/ijms17070797 PMid:27347925 DOI: https://doi.org/10.3390/ijms17070797

Umamaheswari M, Ajith PM, Asokkumar K, Thirumalaisamy S, Jagannath P, Subhadradevi V, et al. In vitro angiotensin converting enzyme inhibitory and antioxidant activities of seed extract of Apium graveolens Linn. Ann Biol Res. 2012;3(3):1274-82.

Aluko RE. Structure and function of plant protein-derived antihypertensive peptides. Curr Opin Food Sci. 2015;4:44-50. https://doi.org/10.1016/j.cofs.2015.05.002 DOI: https://doi.org/10.1016/j.cofs.2015.05.002

Lestari P, Mahayasih PG, Katrin, Rissyelly. Inhibition activity of angiotensin converting enzyme (ACE) and determination of total phenolic and flavonoid compound from bitter melon leaves (Momordica charantia L.). Pharmacogn J. 2017;9(2):252-6. https://doi.org/10.5530/pj.2017.2.43 DOI: https://doi.org/10.5530/pj.2017.2.43

Hettihewa SK, Hemar Y, Rupasinghe HP. Flavonoid-rich extract of Actinidia macrosperma (a wild Kiwifruit) inhibits angiotensin-converting enzyme in vitro. Foods. 2018;7(9):146. https://doi.org/10.3390/foods7090146 PMid:30189590 DOI: https://doi.org/10.3390/foods7090146

Ahmad I, Yanuar A, Mulia K, Mun’im A. Review of angiotensin-converting enzyme inhibitory assay: Rapid method in drug discovery of herbal plants. Pharmacogn Rev. 2017;11(21):1. https://doi.org/10.4103/phrev.phrev_45_16 DOI: https://doi.org/10.4103/phrev.phrev_45_16

Mun’im A, Nurpriantia S, Setyaningsih R, Syahdi RR. Optimization of microwave-assisted extraction of active compounds, antioxidant activity and angiotensin converting enzyme (ACE) inhibitory activity from Peperomia pellucida (L.) Kunth. J Young Pharma. 2017;9(1):S73-8. https://doi.org/10.5530/jyp.2017.1s.19 DOI: https://doi.org/10.5530/jyp.2017.1s.19

Ahmad I, Kurniawan A, Saputri FC, Rissyelly, Mun’im A. Isolation of Angiotensin Converting Enzyme (ACE) Inhibitory Activity Quercetin from Peperomia pellucida. Indonesia: Universitas Mulawarman; 2016. Available from: https://www.repository.unmul.ac.id/handle/123456789/1708 [Last accessed on 2022 Jun 21].

Men R, Li N, Xing Y, Tang Y, Tan C, Meng F, et al. Chemical constituents and ACE inhibitory activity of desert plant Suaeda physophora pall. Acta Pharm Sin B. 2013;3(5):328-32. https://doi.org/10.1016/j.apsb.2013.07.003 DOI: https://doi.org/10.1016/j.apsb.2013.07.003

Balasuriya BW, Rupasinghe HP. Plant flavonoids as angiotensin converting enzyme inhibitors in regulation of hypertension. Funct Foods Health Dis. 2011;1(5):172-88. https://doi.org/10.31989/ ffhd.v1i5.132 DOI: https://doi.org/10.31989/ffhd.v1i5.132

Sholikhah A, Dian FA, Listyorini D. Anatomy and Morphological Study of Mentigi Gunung (Vaccinium varingiaefolium (Blume) Miq.) in Area of Mount Batok-Indonesia. In: International Conference on Biological Science; 2015. p. 36-45. https://doi.org/10.18502/kls.v3i4.685 DOI: https://doi.org/10.18502/kls.v3i4.685

Santoni A, Darwis D, Syahri S. Isolasi Antosianin Dari Buah Pucuk Merah (Syzygium campanulatum Korth.) Serta Pengujian Antioksidan Dan Aplikasi Sebagai Pewarna Alami. Vol. 1. Prosiding Seminar SEMIRATA FMIPA; 2013.

Jena M, Jena J, Biswal SB, Mishra S, Pal A. Effect of Eclipta alba on fructose induced hypertension in albino rats. Int J Pharm Pharm Sci. 2013;5(3):281-5.

Timalsina D, Devkota HP. Eclipta prostrata (L.) L.(Asteraceae): Ethnomedicinal uses, chemical constituents, and biological activities. Biomolecules. 2021;11(11):1738. https://doi.org/10.3390/biom11111738 PMid:34827736 DOI: https://doi.org/10.3390/biom11111738

Shaikh JR, Patil MK. Qualitative tests for preliminary phytochemical screening: An overview. Int J Chem Stud. 2020;8(2):603-8. https://doi.org/10.22271/chemi.2020.v8.i2i.8834 DOI: https://doi.org/10.22271/chemi.2020.v8.i2i.8834

Chen J, Wang Y, Ye R, Wu Y, Xia W. Comparison of analytical methods to assay inhibitors of angiotensin I-converting enzyme. Food Chem. 2013;141:3329-34. https://doi.org/10.1016/j.foodchem.2013.06.048 PMid:23993489 DOI: https://doi.org/10.1016/j.foodchem.2013.06.048

Khan MY, Kumar V. Mechanism and inhibition kinetics of bioassay-guided fractions of Indian medicinal plants and foods as ACE inhibitors. J Tradit Complement Med. 2018;9(1):73-84. https://doi.org/10.1016/j.jtcme.2018.02.001 PMid:30671369 DOI: https://doi.org/10.1016/j.jtcme.2018.02.001

Yaricsha CA, Rissyelly, Katrin. ACE inhibitory activity, total phenolic and flavonoid content of watercress (Nasturtium officinale R. Br.) extract. Pharmacogn J. 2017;9(2):249-51. https://doi.org/10.5530/pj.2017.2.42 DOI: https://doi.org/10.5530/pj.2017.2.42

Mamilla RK, Mishra VK. Effect of germination on antioxidant and ACE inhibitory activities of legumes. LWT. 2017;75:51-8. https://doi.org/10.1016/j.lwt.2016.08.036 DOI: https://doi.org/10.1016/j.lwt.2016.08.036

Gailea R, Bratawinata AA, Pitopang R, Kusuma I. The use of various plant types as medicines by local community in the enclave of the Lore-Lindu national park of Central Sulawesi, Indonesia. Glob J Res Med Plants Indig Med. 2016;5(1):29.

Gunarti NS. Study of ethnobotany of medicinal plants in kutalanggeng and Kutamaneuh villages, Tegalwaru district, Karawang regency, West Java. Majalah Farmasetika. 2016;6(Suppl 1):14-23. https://doi.org/10.24198/mfarmasetika.v6i0.36668 DOI: https://doi.org/10.24198/mfarmasetika.v6i0.36668

Alvionita A, Lambui O, Pitopang R. Study of medicine plants etnobotany topo uma tribe community in Berdikari Village, Sigi Regency, Central Sulawesi. Biocelebes. 2020;14(2):105-18. https://doi.org/10.22487/bioceb.v14i2.15261 DOI: https://doi.org/10.22487/bioceb.v14i2.15261

De Pascual-Teresa S. Molecular mechanisms involved in the cardiovascular and neuroprotective effects of anthocyanins. Arch Biochem Biophys. 2014;559:68-74. https://doi.org/10.1016/j.abb.2014.04.012 PMid:24791600 DOI: https://doi.org/10.1016/j.abb.2014.04.012

Lee C, Han D, Kim B, Baek N, Baik BK. Antioxidant and anti-hypertensive activity of anthocyanin-rich extracts from hulless pigmented barley cultivars. Int J Food Sci Technol. 2013;48(5):984-91. https://doi.org/10.1111/ijfs.12050 DOI: https://doi.org/10.1111/ijfs.12050

Mans DR. Surinamese medicinal plants for treating hypertension with angiotensin-converting enzyme-inhibitory activity: A review of literature. Int J Med Res Health Sci. 2018;7(11):26-48.

Bulea M, Khanb F, Niazd K. Flavonoids (flavones, flavonols, flavanones, flavanonols, flavanols or flavan-3-ols, isoflavones, anthocyanins, chalcones/coumestans). 1st ed. Amsterdam: Elsevier; 2020. p 42-56.

Balasuriya N, Rupasinghe HP. Antihypertensive properties of flavonoid-rich apple peel extract. Food Chem. 2012;135(4):2320-5. https://doi.org/10.1016/j.foodchem.2012.07.023 PMid:22980808 DOI: https://doi.org/10.1016/j.foodchem.2012.07.023

Sakaida H, Nagao K, Higa K, Shirouchi B, Inoue N, Hidaka F, et al. Effect of Vaccinium ashei reade leaves on angiotensin converting enzyme activity in vitro and on systolic blood pressure of spontaneously hypertensive rats in vivo. Biosci Biotechnol Biochem. 2007;71(9):2335-7. https://doi.org/10.1271/bbb.70277 DOI: https://doi.org/10.1271/bbb.70277

Rinayanti A, Radji M, Mun’im A, Suyatna FD. Screening angiotensin converting enzyme (ACE) inhibitor activity of antihypertensive medicinal plants from Indonesia. Int J Pharm Teach Pract. 2013;4(1):527-32.

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Published

2022-08-27

How to Cite

1.
Sandhiutami NMD, Dewi RS, Rahma F, Yang F. Potential Use of Some Indonesian Plants to Inhibits Angiotensin-converting Enzyme In Vitro. Open Access Maced J Med Sci [Internet]. 2022 Aug. 27 [cited 2024 Mar. 28];10(A):1571-6. Available from: https://oamjms.eu/index.php/mjms/article/view/10251