Role of Neurogenesis and Oxidative Stress in Epilepsy (Study on Plasma Brain Derived Neurotrophic Factor and Malondialdehyde Level)

Adelgrit Trisia; Nurul Hidayah; Meitria Syahadatina  Noor; Edi Hartoyo; Indra Widjaja Himawan

doi:10.3889/oamjms.2023.10277

Authors

Adelgrit Trisia Children’s Health Specialist Medical Education Study Program, Faculty of Medicine, Universitas Lambung Mangkurat, Banjarmasin, South Kalimantan, Indonesia
Nurul Hidayah Children’s Health Specialist Medical Education Study Program, Faculty of Medicine, Universitas Lambung Mangkurat, Banjarmasin, South Kalimantan, Indonesia
Meitria Syahadatina Noor Public Health Study Program Master Program, Faculty of Medicine, Universitas Lambung Mangkurat, Banjarbaru, South Kalimantan, Indonesia https://orcid.org/0000-0001-8406-6055
Edi Hartoyo Children’s Health Specialist Medical Education Study Program, Faculty of Medicine, Universitas Lambung Mangkurat, Banjarmasin, South Kalimantan, Indonesia
Indra Widjaja Himawan Children’s Health Specialist Medical Education Study Program, Faculty of Medicine, Universitas Lambung Mangkurat, Banjarmasin, South Kalimantan, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2023.10277

Keywords:

Epilepsy, Brain derived neurotrophic factor, Malondialdehyde

Abstract

BACKGROUND: Epilepsy is a neurological disorder. Its incidence in Indonesia was 700,000–1,400,000 cases and 40–50% occurred in children. About 30–40% of cases in children had uncontrolled seizures. Biomarkers are needed to assess the prognostic value of patients with uncontrolled epilepsy. Malondialdehyde (MDA) and brain-derived neurotrophic factor (BDNF) are one of the prognostic biomarkers related to uncontrolled epilepsy to see the effect of oxidative stress and neuroplasticity.

AIM: The objective of the study was to examine cut off value of plasma BDNF and MDA level; and to compare plasma BDNF and MDA levels in uncontrolled and controlled epilepsy patients.

METHODS: The research usedanalytic observational with cross-sectional approach. Number of respondents was 30 patients of epilepsy who came to Ulin Hospital Banjarmasin. Respondents were divided into two groups (controlled and uncontrolled epilepsy). Blood plasma was examined for MDA with a spectrophotometer and BDNF with ELISA. Data were analyzed by t-test with 95% confidence level.

RESULTS: 11 children were found in the uncontrolled epilepsy group and 19 children with controlled epilepsy. The result showed that there were significant differences between type of therapy and developmental disorders/other diseases with epilepsy status. There was no significant differences of plasma BDNF in epilepsy status (controlled and uncontrolled epilepsy), and there was also no significant differences of plasma MDA in epilepsy status (controlled and uncontrolled epilepsy).

CONCLUSION: There were no significant differences of plasma BDNF and MDA in epilepsy status.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Plum Analytics Artifact Widget Block

References

Fisher RS, Cross JH, D’Souza C, French JA, Haut SR, Higurashi N, et al. Instruction manual for the ILAE 2017 operational classification of seizure types. Epilepsia. 2017;58(4):531-42. https://doi.org/10.1111/epi.13671 PMid:28276064 DOI: https://doi.org/10.1111/epi.13671

Abass BA, Mohamed IN, Elseed MA, Karrar ZA. Causes of uncontrolled seizures among Sudanese children. Khartoum Med J. 2014;7(1):915-20.

Farghaly WM, El-Tallawy HN, Rageh TA, Mohamed EM, Metwally NA, Shehata GA, et al. Epidemiology of uncontrolled epilepsy in the Al-Kharga district, New Valley, Egypt. Seizure. 2013;22(8):611-6. https://doi.org/10.1016/j.seizure.2013.04.010 PMid:23680080 DOI: https://doi.org/10.1016/j.seizure.2013.04.010

Sperling MR. The consequences of uncontrolled epilepsy. CNS Spectr. 2004;9(2):98-109. https://doi.org/10.1017/s1092852900008464 PMid:14999166 DOI: https://doi.org/10.1017/S1092852900008464

Tang F, Hartz AM, Bauer B. Drug-resistant epilepsy: Multiple hypotheses, few answers. Front Neurol. 2017;8:301. https://doi.org/10.3389/fneur.2017.00301 PMid:28729850 DOI: https://doi.org/10.3389/fneur.2017.00301

Asadi-Pooya AA, Emami M, Ashjazadeh N, Nikseresht A, Shariat A, Petramfar P, et al. Reasons for uncontrolled seizures in adults; The impact of pseudointractability. Seizure. 2013;22(4):271-4. https://doi.org/10.1016/j.seizure.2013.01.010 PMid:23375939 DOI: https://doi.org/10.1016/j.seizure.2013.01.010

Chintia NP, Wijayanti IA, Mahalini DS. The relationship of antiepileptic drug therapy to cognitive function in pediatric epilepsy patients at Sanglah Central Public Hospital, March 2016-November 2016. J Medika Udayana. 2020;9(7):64-9. https://doi.org/10.24843/10.24843.MU.2020.V09.i7.P13

RSUD Ulin Banjarmasin. Pusat Data Elektronik. Banjarmasin: RSUD Ulin Banjarmasin. 2019.

Beghi E. Global, regional, and national burden of epilepsy, 1990-2016: A systematic analysis for the global burden of disease study 2016. Lancet Neurol. 2019;18(4):357-75. https://doi.org/10.1016/S1474-4422(18)30454-X PMid:30773428 DOI: https://doi.org/10.1016/S1474-4422(18)30454-X

Menon B, Ramalingam K, Kumar RV. Oxidative stress in patients with epilepsy is independent of antiepileptic drugs. Seizure. 2012;21(10):780-4. https://doi.org/10.1016/j.seizure.2012.09.003 PMid:23031823 DOI: https://doi.org/10.1016/j.seizure.2012.09.003

Syafrita Y, Amir D, Susanti R, Fadhilah I. Relationship of brain-derived neurotrophic factor, malondialdehyde, and 8-Hydroxy 2-Deoxyguanosine with post-ischemic stroke depression. Dement Neuropsychol. 2020;14(1):41-6. https://doi.org/10.1590/1980-57642020dn14-010007 PMid:32206197 DOI: https://doi.org/10.1590/1980-57642020dn14-010007

Alcantara JA, Vincentiis S, Santos B, Kerr D, de Paula V, Alessia R, et al. BDNF Val66Met polymorphism is not related with temporal lobe epilepsy caused by hippocampal sclerosis in Brazilian population. Seizure. 2018;60:159-62. https://doi.org/10.1016/j.seizure.2018.07.004 PMid:30015148 DOI: https://doi.org/10.1016/j.seizure.2018.07.004

Notaras M, van den Buuse M. Brain-derived neurotrophic factor (BDNF): Novel insights into regulation and genetic variation. Neuroscientist. 2019;25(5):434-54. https://doi.org/10.1177/1073858418810142 PMid:30387693 DOI: https://doi.org/10.1177/1073858418810142

Hong Z, Li W, Qu B, Zou X, Chen J, Sander JW, et al. Serum brain-derived neurotrophic factor levels in epilepsy. Eur J Neurol. 2014;21(1):57-64. https://doi.org/10.1111/ene.12232 PMid:23879572 DOI: https://doi.org/10.1111/ene.12232

Luef G, Tauboll E. Gender issues in epilepsy-difference in management of epilepsy. Seizure. 2015;28:1-2. https://doi.org/10.1016/j.seizure.2015.02.001 PMid:25802125 DOI: https://doi.org/10.1016/j.seizure.2015.02.001

Hu Y, Shan Y, Du Q, Ding Y, Shen C, Wang S, et al. Gender and socioeconomic disparities in global burden of epilepsy: An analysis of time trends from 1990 to 2017. Front Neurol. 2021;12:643450. https://doi.org/10.3389/fneur.2021.643450 PMid:33935942 DOI: https://doi.org/10.3389/fneur.2021.643450

Minardi C, Minacapelli R, Valastro P, Vasile F, Pitino S, Pavone P, et al. Epilepsy in children: From diagnosis to treatment with focus on emergency. J Clin Med. 2019;8:39. https://doi.org/10.3390/jcm8010039 PMid:30609770 DOI: https://doi.org/10.3390/jcm8010039

Pavone P, Gulizia C, Pira AL, Greco F, Parisi P, Cara GD, et al. Cerebral palsy and epilepsy in children: Clinical perspectives on a common comorbidity. Children (Basel). 2021;8(1):16. https://doi.org/10.3390/children8010016 PMid:33396243 DOI: https://doi.org/10.3390/children8010016

Hansen SL, Lorentzen J, Pedersen LT, Hendrich FL, Jorsal M, Pingel J, et al. Suboptimal nutrition and low physical activity are observed together with reduced plasma brain-derived neurotrophic factor (BDNF) concentration in children with severe cerebral palsy (CP). Nutrients. 2019;11(3):620. https://doi.org/10.3390/nu11030620 PMid:30875771 DOI: https://doi.org/10.3390/nu11030620

Flora R, Juhaina E, Faisya AF, Fajar NA, Appulembang YA, Zulkarnain M. Brain-derived neurotrophic factor (BDNF) serum and intelligence levels of elementary school children in rural areas, Seluma Regency. J Ilmu Kesehatan Masyarakavt. 2021;12(1):60-8. https://doi.org/10.26553/jikm.2021.12.1.60-68 DOI: https://doi.org/10.26553/jikm.2021.12.1.60-68

Sjahrir M. Hubungan Kadar Brain-Derived Neurotrophic Factor Dalam Serum Dengan Derajat Gejala Depresi Pada Penderita Psoriasis Vulgaris. Indonesia: Fakultas Kedokteran Universitas Sumatera Utara; 2018. p. 1-92

Setiawan I, Harsono H, Asmedi A. EEG awal terapi sebagai prediktor kekambuhan pada penderita epilepsi yang mendapat terapi obat antiepilepsi. Biomedika. 2018;10(1):15-20. https://doi.org/10.23917/biomedika.v10i1.5849 DOI: https://doi.org/10.23917/biomedika.v10i1.5849

Chintia NP, Wijayanti IA, Mahalini DS. The relationship of antiepileptic drug therapy to cognitive function in pediatric epilepsy patients at Sanglah General Hospital in the period March-November 2016. J Medika Udayana. 2020;9(7):64-9. https://doi.org/10.24843/10.24843.MU.2020.V09.i7.P13

Suwarba IG. Incidence and clinical characteristics of epilepsy in children. Sari Pediatri. 2011;13(2):123-8. https://doi.org/10.14238/sp13.2.2011.123-8 DOI: https://doi.org/10.14238/sp13.2.2011.123-8

Silakarma D, Sudewi AA. The role of brain-derived neurotrophic factor (BDNF) in cognitive functions. Bali Med J. 2019;8(2):427-34. https://doi.org/10.15562/bmj.v8i2.1460 DOI: https://doi.org/10.15562/bmj.v8i2.1460

Martínez-Levy GA, Rocha L, Lubin FD, Alonso-Vanegas MA, NaniA, Buentello-García RM, et al. Increased expression of BDNF transcript with exon VI in hippocampi of patients with pharmaco resistant temporal lobe epilepsy. Neuroscience. 2016;314:12-26. https://doi.org/10.1016/j.neuroscience.2015.11.046 PMid:26621122 DOI: https://doi.org/10.1016/j.neuroscience.2015.11.046

Bathina S, Das UN. Brain-derived neurotrophic factor and its clinical implications. Arch Med Sci. 2015;11(6)1165-78. https://doi.org/10.5114/aoms.2015.56342 PMid:26788077 DOI: https://doi.org/10.5114/aoms.2015.56342

Giacobbo BL, Doorduin J, Klein HC, Dierckx RA, Bromberg E, VriesEF. Brain-derived neurotrophic factor in brain disorders: Focus on neuroinflammation. Mol Neurobiol. 2019;56(5):3295-312. https://doi.org/10.1007/s12035-018-1283-6 PMid:30117106 DOI: https://doi.org/10.1007/s12035-018-1283-6

Naegelin Y, Dingsdale H, Säuberli K, Schädelin S, Kappos L, Barde YA. Measuring and validating the levels of brain derived neurotrophic factor in human serum. eNeuro. 2018;5(2):1-9. https://doi.org/10.1523/ENEURO.0419-17.2018 PMid:29662942 DOI: https://doi.org/10.1523/ENEURO.0419-17.2018

Sidyanto T, Hermanto TJ. Comparison of brain derived neurotrophic factors (BDNF) serum cord blood levels of newborns between pregnant women who received DHA with a combination of DHA and 11-14 by Mozart during pregnancy. Majalah Obstetri dan Ginekologi. 2013;21(3):109-14.

Lorigados L, Morales LM, Orozco-Suárez S, Gallardo JM, Díaz-Hung ML, González ME, et al. Oxidative stress in pharmacoresistant epilepsy. Biotecnol Apl. 2016;33:2101-7.

Stafstrom CE. The pathophysiology of epileptic seizures: A primer for pediatricians. Pediatr Rev. 1998;19(10):342-251. https://doi.org/10.1542/pir.19-10-342 PMid:9785934 DOI: https://doi.org/10.1542/pir.19.10.342