The Effect of Methanol Concentration on the Extraction of Moringa Leaf (Moringa oleifera) and Papaya Fruit (Carica papaya) on Elastase and Hyaluronidase Installing Activities
DOI:
https://doi.org/10.3889/oamjms.2022.10302Keywords:
Moringa oleifera, Carica papaya, Methanol, Elastase, HyaluronidaseAbstract
Abstract
BACKGROUND OF THE STUDY: Β-carotene, flavonoids and phenolic compounds found in methanol extracts of Moringa leaves and papaya fruit have high antioxidant activity so that they can be used for antiaging. The concentration of solvent is a factor that will affect the levels of active ingredients in methanol extract. This study aims to determine the effect of the concentration of the methanol solvent derived from the extraction of Moringa leaves and papaya fruit on the inhibitory activity of the elastase and hyaluronidase enzymes.
AIM OF THE STUDY: Knowing the best extracts of methanol 50, 70, and 96% of Moringa leaf (Moringa oleifera) and papaya fruit (Carica papaya) as antiaging agents through inhibition of elastase and hyaluronidase enzymes.
METHODOLOGY: In this study variations in the concentration of methanol 50%, 70%, and 96% were used for the extraction of Moringa leaves and papaya fruit. The extract was obtained by maceration method which was then tested for the inhibition of the enzyme activity of elastase and hyaluronidase using ELISA (Enzyme-Linked Immunosorbent Assay).
RESULTS: The results of the elastase enzyme inhibitory activity test on Moringa leaf extract with 50%, 70%, and 96% methanol solvents IC50 respectively = 9453.38; 6604.70; 12346.44 μg / mL, while the yield of papaya extract 5995.31; 9046.25; 11571.54 μg / mL. In addition, the test results showed the inhibitory activity of the hyaluronidase enzyme showed that the Moringa leaf extract with 50%, 70%, and 96% solvents IC50 respectively = 2944.53; 1028,36; 3001.83 μg / mL, while the yield of methanol extract of papaya fruit is 982.67; 2982.96; 3530.18 μg / mL.
CONCLUSION: Based on the test results it can be concluded that the most effective solvent concentration as an inhibition of the enzyme elastase and hyaluronidase methanolic extract of Moringa leaves is 70% methanol solvent. While papaya fruit methanol extract which is effective in inhibiting the enzymes elastase and hyaluronidase is 50% methanol solvent.
Downloads
Metrics
Plum Analytics Artifact Widget Block
References
Widowati W, Fauziah N, Herdiman H, Afni M, Afifah E, Kusuma HS, et al. Antioxidant and anti aging assays of Oryza sativa extracts, vanillin and coumaric acid. J Nat Rem. 2016;16(3):88-99. https://doi.org/10.18311/jnr/2016/7220 DOI: https://doi.org/10.18311/jnr/2016/7220
Liyanaarachchi GD, Samarasekera JK, Mahanama KR, Hemalal KD. Tyrosinase, elastase, hyaluronidase, inhibitory and antioxidant activity of Sri Lankan medicinal plants for novel cosmeceuticals. Ind Crops Prod. 2018;111:597-605. https://doi.org/10.1016/j.indcrop.2017.11.019 DOI: https://doi.org/10.1016/j.indcrop.2017.11.019
Parwata MO. Antioksidan. Indonesia: Kim Terap Program Pascasarjana University Udayana; 2016. p. 1-54.
Desmiaty Y, Winarti W, Nursih AM, Nisrina H, Finotory G. Antioxidant and antielastase activity of Kaempferia rotunda and Curcuma zedoaria. Res J Chem Environ. 2018;22(1):95-8.
Tu PT, Tawata S. Anti-oxidant, anti-aging, and anti-melanogenic properties of the essential oils from two varieties of Alpinia zerumbet. Molecules. 2015;20(9):16723-40. https://doi.org/10.3390/molecules200916723 DOI: https://doi.org/10.3390/molecules200916723
Natanael GI, Simorangkir GF, Purba NP, Tambunan MP, Nasution AN, Amansyah A. Potensi Antioksidan Dan Anti-elastase Ekstrak Daun kelor (Moringa oleifera) terhadap antiaging. J Keperawatan Prior. 2021;4:69-76.
Asifa NN, Tasminatun S, Nabawiyata S, Makiyah N. Potential Ethanol Extract of Papaya (Carica papaya L.) as an Anti- Inflammatory Agent through Degree of Duodenum Inflammation in BALB/c. Muhammadiyah University of Yogyakarta. 2018; 4:37–44. DOI: https://doi.org/10.31603/pharmacy.v4i2.2319
Xu Y, Chen G, Guo M. Potential anti-aging components from Moringa oleifera leaves explored by affinity ultrafiltration with multiple drug targets. Front Nutr. 2022;9:854882. https://doi.org/10.3389/fnut.2022.854882 PMid:35619958 DOI: https://doi.org/10.3389/fnut.2022.854882
Chattuwatthana T, Okello E. Anti-collagenase, Anti-elastase and antioxidant activities of Pueraria candollei var. mirifica root extract and Coccinia grandis fruit juice extract: An in vitro study. Eur J Med Plants. 2015;5(4):318-27. https://doi.org/10.9734/ejmp/2015/14129 DOI: https://doi.org/10.9734/EJMP/2015/14129
Vijayakumar R, Gani SS, Mokhtar NF. Anti-elastase, anti-collagenase and antimicrobial activities of the underutilized red pitaya peel: An in vitro study for anti-aging applications. Asian J Pharm Clin Res. 2017;10(8):251-5. https://doi.org/10.22159/ajpcr.2017.v10i8.19048 DOI: https://doi.org/10.22159/ajpcr.2017.v10i8.19048
Downloads
Published
How to Cite
License
Copyright (c) 2022 Nining Sugihartini, Mohamad Usman Nur, Sapto Yuliani (Author)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
http://creativecommons.org/licenses/by-nc/4.0
