Analysis of Helicobacter pylori in Saliva of Patients with Laryngopharyngeal Reflux and Non-Laryngopharyngeal Reflux

Ade Asyari; Eti Yerizel; Andani Eka Putra; Firdawati Firdawati; Refi Amalia Utami

doi:10.3889/oamjms.2022.10407

Authors

Ade Asyari Department of Otorhinolaryngology, Head and Neck Surgery, Faculty of Medicine, Andalas University, Padang, Indonesia
Eti Yerizel Department of Biochemistry, Faculty of Medicine, Andalas University, Padang, Indonesia
Andani Eka Putra Department of Microbiology, Faculty of Medicine, Andalas University, Padang, Indonesia
Firdawati Firdawati Departement of Public Health, Faculty of Medicine, Andalas University, Padang, Indonesia
Refi Amalia Utami Faculty of Medicine, Andalas University, Padang, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2022.10407

Keywords:

Helicobacter pylori, Laryngopharyngeal reflux, Real time-polymerase chain reaction

Abstract

BACKGROUND : Helicobacter pylori is a gram-negative bacteria known as the causative agent of chronic gastritis, peptic ulcer, gastric adenocarcinoma, and gastric mucosa-associated lymphoid tissue lymphoma. Several studies have correlated H. pylori in the pathogenesis of upper airway diseases. H. pylori can be detected in saliva, oropharyngeal aphthae, nasal and sinus mucosa, secretions from the tympanic cavities, larynx, and pharyngeal lymphoid tissue. The diagnosis of LPR can be made simply by examining saliva.

AIM : The aim of the study is to analyze the presence of H.pylori in the saliva of LPR and non LPR patients.

METHODS : This study is an analytic observational study with a case control design. The research was conducted in the ENT-KL Department of Dr. M. Djamil Hospital, Padang, Indonesia. PCR examination was carried out at the Biomedical Laboratory of the Faculty of Medicine, Andalas University on saliva samples to detect the presence of H.Pylori. The study was conducted on 22 LPR patients and 22 control subjects.

RESULT : LPR patients are more common in women than men, which were 12 women and 10 men. The average age of LPR patients is 43.7 years. The presence of H.pylori in LPR patients was 86.4%, while in the non-LPR group was 50%. After being tested using the Chi-square test, a significant difference was found between the presence of H. Pylori and the incidence of LPR (P = 0.010).

CONCLUSION : There was an increase in the frequency of the presence of Helicobacter Pylori in patients with LPR compared to Non LPR patients after statistical analysis.

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