Single Nucleotide Polymorphism of Dectin-1 Gene Associates with Atopic Dermatitis in Children

Authors

  • Olexandr Volosovets Department of Pediatrics, O. O. Bogomolets National Medical University, Kyiv, Ukraine
  • Sergii Kryvopustov Department of Pediatrics, O. O. Bogomolets National Medical University, Kyiv, Ukraine
  • Olena Mozyrska Department of Pediatrics, O. O. Bogomolets National Medical University, Kyiv, Ukraine
  • Sergii Goncharov Department of General and Molecular Pathophysiology, O. O. Bogomoletz Institute of Physiology of the National Academy of Sciences of Ukraine, Sector of Molecular Physiology, Kyiv Ukraine Abstract
  • Anna Kupkina Department of Pediatrics, O. O. Bogomolets National Medical University, Kyiv, Ukraine
  • Oksana Iemets Department of Pediatrics, O. O. Bogomolets National Medical University, Kyiv, Ukraine
  • Victor Dosenko Department of General and Molecular Pathophysiology, O. O. Bogomoletz Institute of Physiology of the National Academy of Sciences of Ukraine, Sector of Molecular Physiology, Kyiv Ukraine

DOI:

https://doi.org/10.3889/oamjms.2022.10513

Keywords:

SNP, Dectin-1, Malassezia, Atopic dermatitis, Children

Abstract

BACKGROUND: Atopic dermatitis (AD) is a chronic inflammatory skin disease with complex and multifactorial pathophysiology, involving elements of barrier dysfunction, alterations in cell-mediated immune responses, IgE sensitization, and environmental factors. This encourages the search for predictors of disease development among both genetic markers and environment.

AIM: The aim of the study was to examine if genetic factors of Malassezia recognition, or Malassezia colonization may be related to IgE sensitization or to severity of AD.

METHODS: The study included 106 patients with eczema and 103 healthy children. Specific IgE against Malassezia mix (m227) was analyzed in 51 patients using immunochemiluminescent method on the ImmunoCAP 100 (Thermo Fisher Scientific Inc., Phadia, Sweden). Genotyping for rs7309123 in Dectin-1 was performed using Real-time PCR. The level of colonization by Malassezia in the scale samples was determined by a real-time PCR assay.

RESULTS: Increased IgE to Malassezia spp. was observed in 29,4% of children with eczema. Higher Malassezia spp. – specific IgE titer positively correlated with severity of AD, age of onset, head–neck type of AD, and a higher total IgE. GG genotype rs7309123 Dectin-1 is significantly more often found in the patients than in the control group, but no correlation with IgE sensitization to Malassezia was found. Malassezia restricta and M. globosa were predominant in patients and controls, with some predominance of M. globosa over M. restricta among patients.

CONCLUSION: Sensitization to Malassezia, genetic markers in Dectin-1, and Malassezia colonization of the skin can be tools for studying the gene-environment interactions in the pathogenesis of AD.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Plum Analytics Artifact Widget Block

References

Corzo-León DE, MacCallum DM, Munro CA. Host responses in an Ex vivo human skin model challenged with Malassezia sympodialis. Front Cell Infect Microbiol. 2021;10:561382. https://doi.org/10.3389/fcimb.2020.561382 PMid:33552997 DOI: https://doi.org/10.3389/fcimb.2020.561382

Lange L, Alter N, Keller T, Rietschel E. Sensitization to Malassezia in infants and children with atopic dermatitis: Prevalence and clinical characteristics. Allergy. 2008;63(4):486-87. https://doi.org/10.1111/j.1398-9995.2007.01623.x PMid:18315742 DOI: https://doi.org/10.1111/j.1398-9995.2007.01623.x

Kekki OM, Scheynius A, Poikonen S, Koskinen A, Kautiainen H, Turjanmaa K. Sensitization to Malassezia in children with atopic dermatitis combined with food allergy. Pediatr Allergy Immunol. 2013;24(3):244-9. https://doi.org/10.1111/pai.12057 PMid:23551180 DOI: https://doi.org/10.1111/pai.12057

Glatz M, Bosshard PP, Hoetzenecker W, Schmid-Grendelmeier P. The role of Malassezia spp. In atopic dermatitis. J Clin Med. 2015;4(6):1217-28. https://doi.org/10.3390/jcm4061217 PMid:26239555 DOI: https://doi.org/10.3390/jcm4061217

Brodská P, Panzner P, Pizinger K, Schmid-Grendelmeier P. IgE-mediated sensitization to Malassezia in atopic dermatitis: More common in male patients and in head and neck type. Dermatitis. 2014;25(3):120-6. https://doi.org/10.1097/DER.0000000000000040 PMid:24819285 DOI: https://doi.org/10.1097/DER.0000000000000040

Sandström Falk MH, Faergemann J. Atopic dermatitis in adults: Does it disappear with age? Acta Derm Venereol. 2006;86(2):135-9. https://doi.org/10.2340/00015555-0040 PMid:16648916

Glatz M, Buchner M, von Bartenwerffer W, Schmid-Grendelmeier P, Worm M, Hedderich J, et al. Malassezia spp. Specific immunoglobulin E level is a marker for severity of atopic dermatitis in adults. Acta Derm Venereol. 2015;95:191-6. https://doi.org/10.2340/00015555-1864 PMid:24696225 DOI: https://doi.org/10.2340/00015555-1864

Ramirez de Knott HM, McCormick TS, Kalka K, Skandamis G, Ghannoum MA, Schluchter M, et al. Cutaneous hypersensitivity to Malassezia sympodialis and dust mite in adult atopic dermatitis with a textile pattern. Contact Dermatitis. 2006;54(2):92-9. https://doi.org/10.1111/j.0105-1873.2006.00774.x PMid:16487281 DOI: https://doi.org/10.1111/j.0105-1873.2006.00774.x

Joo H, Upchurch K, Zhang W, Ni, Li D, Xue Y, et al. Opposing roles of dectin-1 expressed on human plasmacytoid dendritic cells and myeloid dendritic cells in Th2 polarization. J Immunol. 2015;195(4):1723-31. https://doi.org/10.4049/jimmunol.1402276 PMid:26123355 DOI: https://doi.org/10.4049/jimmunol.1402276

Nathan AT, Peterson EA, Chakir J, Wills-Karp M. Innate immune responses of airway epithelium to house dust mite are mediated through beta-glucan-dependent pathways. J Allergy Clin Immunol. 2009;123(3):612-8. https://doi.org/10.1016/j.jaci.2008.12.006 PMid:19178937 DOI: https://doi.org/10.1016/j.jaci.2008.12.006

Chen MJ, Hu R, Jiang XY, Wu Y, He ZP, Chen ZY, et al. Dectin-1 rs3901533 and rs7309123 polymorphisms increase susceptibility to pulmonary invasive fungal disease in patients with acute myeloid leukemia from a Chinese Han population. Curr Med Sci. 2019;39(6):906-12. https://doi.org/10.1007/s11596-019-2122-3 PMid:31845221 DOI: https://doi.org/10.1007/s11596-019-2122-3

Williams HC, Burney PG, Hay RJ, Archer CB, Shipley MJ, Hunter JJ, et al. The U.K. working party’s diagnostic criteria for atopic dermatitis. I. Derivation of a minimum set of discriminators for atopic dermatitis. Br J Dermatol. 1994;131(3):383-96. https://doi.org/10.1111/j.1365-2133.1994.tb08530.x PMid:7918015 DOI: https://doi.org/10.1111/j.1365-2133.1994.tb08530.x

Severity scoring of atopic dermatitis: The SCORAD index. Consensus report of the European task force on atopic dermatitis. Dermatology. 1993;186(1):23-31. https://doi.org/10.1159/000247298 PMid:8435513 DOI: https://doi.org/10.1159/000247298

Sugita T, Suzuki M, Goto S, Nishikawa A, Hiruma M, Yamazaki T, et al. Quantitative analysis of the cutaneous Malassezia microbiota in 770 healthy Japanese by age and gender using a real-time PCR assay. Med Mycol. 2010;48(2):229-33. https://doi.org/10.1080/13693780902977976 PMid:19462267 DOI: https://doi.org/10.3109/13693780902977976

Volosovets ОP, Bolbot YK, Beketova GV, Berezenko VS, Umanets TR, Rechkina OO, et al. Allergic march in children of Ukraine. Medicni Perspektivi. 2021;26(4):181-8. https://doi.org/10.26641/2307-0404.2021.4.248227 DOI: https://doi.org/10.26641/2307-0404.2021.4.248227

Johansson C, Sandström MH, Bartosik J, Särnhult T, Christiansen J, Zargari A, et al. Atopy patch test reactions to Malassezia allergens differentiate subgroups of atopic dermatitis patients. Br J Dermatol. 2003;148:479-88. https://doi.org/10.1046/j.1365-2133.2003.05093.x PMid:12653739 DOI: https://doi.org/10.1046/j.1365-2133.2003.05093.x

Guglielmo A, Sechi A, Patrizi A, Gurioli C, Neri I. Head and neck dermatitis, a subtype of atopic dermatitis induced by Malassezia spp: Clinical aspects and treatment outcomes in adolescent and adult patients. Pediatr Dermatol. 2020;38(1):109-14. https://doi.org/10.1111/pde.14437 PMid:33155738 DOI: https://doi.org/10.1111/pde.14437

Patin EC, Thompson A, Orr SJ. Pattern recognition receptors in fungal immunity. Semin Cell Dev Biol. 2019;89:24-33. https://doi.org/10.1016/j.semcdb.2018.03.003 PMid:29522806 DOI: https://doi.org/10.1016/j.semcdb.2018.03.003

White PL, Parr C, Barnes RA. Predicting invasive aspergillosis in hematology patients by combining clinical and genetic risk factors with early diagnostic biomarkers. J Clin Microbiol. 2017;56(1):e01122-17. https:/doi.org/10.1128/JCM.01122-17 PMid:29118175 DOI: https://doi.org/10.1128/JCM.01122-17

Zhou P, Xie Y, Yan Z, Liu X, Hua H. Association between dectin-1 gene single nucleotide polymorphisms and fungal infection: A systemic review and meta-analysis. Biosci Rep. 2019;39(11):BSR20191519. https://doi.org/10.1042/BSR20191519 PMid:31696220 DOI: https://doi.org/10.1042/BSR20191519

Sainz J, Lupiáñez CB, Segura-Catena J, Vazquez L, Ríos R, Oyonarte S, Hemminki K, et al. Dectin-1 and DC-SIGN polymorphisms associated with invasive pulmonary aspergillosis infection. PLoS One. 2012;7(2):e32273. https://doi.org/10.1371/journal.pone.0032273 PMid:22384201 DOI: https://doi.org/10.1371/journal.pone.0032273

Fischer M, Spies-Weisshart B, Schrenk K, Gruhn B, Wittig S, Glaser A, et al. Polymorphisms of dectin-1 and TLR2 predispose to invasive fungal disease in patients with acute myeloid leukemia. PLoS One. 2016;11(3):e0150632. https://doi.org/10.1371/journal.pone.0150632 PMid:26963509 DOI: https://doi.org/10.1371/journal.pone.0150632

Saad M, Sugita T, Saeed H, Ahmed A. Molecular epidemiology of Malassezia globosa and Malassezia restricta in Sudanese patients with pityriasis versicolor. Mycopathologia. 2013;175(1-2):69-74. https://doi.org/10.1007/s11046-012-9587-y PMid:23054329 DOI: https://doi.org/10.1007/s11046-012-9587-y

Woo YR, Cho M, Han Y, Lee SH, Cho SH, Lee JD, et al.Characterization of distinct microbiota associated with scalp dermatitis in patients with atopic dermatitis. J Clin Med. 2022;11(6):1735. https://doi.org/10.3390/jcm11061735 PMid:35330061 DOI: https://doi.org/10.3390/jcm11061735

Downloads

Published

2022-08-19

How to Cite

1.
Volosovets O, Kryvopustov S, Mozyrska O, Goncharov S, Kupkina A, Iemets O, Dosenko V. Single Nucleotide Polymorphism of Dectin-1 Gene Associates with Atopic Dermatitis in Children. Open Access Maced J Med Sci [Internet]. 2022 Aug. 19 [cited 2024 Mar. 29];10(A):1295-9. Available from: https://oamjms.eu/index.php/mjms/article/view/10513