The Combination of Mesenchymal Stem Cells and Bovine Colostrum in Reducing α-SMA Expression and NLR Levels in Wistar Rats After 50% Fibrotic Liver Resection

Michael Mawi Hartanto; Yan Wisnu Prajoko; Agung Putra; Nur Dina Amalina

doi:10.3889/oamjms.2022.10557

Authors

Michael Mawi Hartanto Department of Biomedical Sciences, Faculty of Medicine, Diponegoro University, Semarang, Indonesia
Yan Wisnu Prajoko Department of Biomedical Sciences, Faculty of Medicine, Diponegoro University, Semarang, Indonesia
Agung Putra Stem Cell and Cancer Research, Faculty of Medicine, Sultan Agung Islamic University, Semarang, Indonesia; Department of Pathology, Medical Faculty, Sultan Agung Islamic University, Semarang, Indonesia; Department of Biomedical Science, Medical Faculty, Sultan Agung Islamic University, Semarang, Indonesia https://orcid.org/0000-0003-4261-9437
Nur Dina Amalina Stem Cell and Cancer Research, Faculty of Medicine, Sultan Agung Islamic University, Semarang, Indonesia; Pharmacy Study Program, Faculty of Mathematics and Natural Sciences, Universitas Negeri, Semarang, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2022.10557

Keywords:

Liver fibrosis, α-SMA, Neutrophil lymphocyte ratio, Mesenchymal stem cells, Bovine colostrum

Abstract

Background: Liver fibrogenesis will produce α-smooth muscle actin (α-SMA) expression and a continuous inflammatory process, seen through the neutrophil lymphocyte ratio (NLR). The combination of mesenchymal stem cells and bovine colostrum is a novel strategy for repairing hepatic fibrosis tissue. To assess the combination of mesenchymal stem cells and bovine colostrum to reduce α-SMA expression and NLR levels in Wistar rats after 50% fibrotic liver resection.

Methods: Thirty-six Wistar male rats were randomly divided into 6 groups (sham, control, colostrum, MSCs, and colostrum and MSCs combination). Rats were injected with CCl₄ for 8 weeks to induce liver fibrosis then underwent liver resection. NLR levels was determined using Hematology Analyzer, α-SMA expression of myofibroblast was analyzed by immunofluorescence staining.

Results: A significant reduction in NLR levels on day 3 in the treatment group I (1.10), treatment II (0.83), treatment III (0.93) compared to the control group. A significant reduction in NLR levels on day 10 in the treatment group I (0.76), treatment II (0.64), treatment III (0.54) compared to the control group. A significant decrease in α-SMA in treatment group I (0.134), treatment II (0.68), treatment III (0.42) compared to the control group.

Conclusion: In this study, it was found that α-SMA expression, NLR levels on the 3rd and 10th day of administration were reduced in group receiving combination of mesenchymal stem cells and bovine colostrum in the liver of post-resection Wistar rats by 50%.

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