Vitamin D Receptor Gene ApaI and TaqI Polymorphisms in Type 2 Diabetes among Saudi Population

Authors

  • Fathy M. Elfasakhany Department of Basic and Clinical Oral Sciences https://orcid.org/0000-0001-5314-1673
  • Mashael S. Alqahtani Department of Basic and Clinical Oral Sciences
  • Ayman M. A. Elguindy Department of Basic and Clinical Oral Sciences, Faculty of Dentistry, Umm Al Qura University, Mecca, Saudi Arabia
  • Magdi A. El-Damarawi Department of Medical Physiology, Faculty of Medicine, Tanta University, Tanta, Egypt https://orcid.org/0000-0003-0271-6849

DOI:

https://doi.org/10.3889/oamjms.2022.10625

Keywords:

Vitamin D, Type 2 diabetes mellitus, Vitamin D receptor, Gene polymorphism, ApaI, TaqI

Abstract

Background: Variants of vitamin D receptor (VDR) gene have been linked to a variety of diseases, including metabolic syndrome, cancer, bone disease, and tuberculosis. The relationship between VDR gene mutations and the susceptibility of type 2 diabetes mellitus (T2DM) in different ethnic groups is yet unknown. Vitamin D and its receptor complex have a function in regulating β-cell insulin secretion as a transcription factor.

Objectives: The goal of this study was to see if there is a link between VDR Apa1 and Taq1 polymorphisms and T2DM susceptibility in the Saudis of Makkah environ.

Materials and Methods: DNA was separated from peripheral blood and genotyped in 110 healthy controls and 110 unrelated people with T2DM for the VDR ApaI (G/T) rs7975232 and TaqI (A/G) rs731236 single nucleotide polymorphisms (SNPs) using polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) technique.

Results: The distributions of genotypes and alleles of VDR ApaI and TaqI polymorphisms were statistically indifferent across the groups investigated (P >0.05).

Conclusion: These findings showed that polymorphisms in the VDR ApaI and TaqI genes may not be linked to T2DM risk in Saudis.

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Published

2022-09-23

How to Cite

1.
Elfasakhany FM, Alqahtani MS, Elguindy AMA, El-Damarawi MA. Vitamin D Receptor Gene ApaI and TaqI Polymorphisms in Type 2 Diabetes among Saudi Population. Open Access Maced J Med Sci [Internet]. 2022 Sep. 23 [cited 2023 Mar. 31];10(A):1520-4. Available from: https://oamjms.eu/index.php/mjms/article/view/10625