Vitamin D Receptor Gene ApaI and TaqI Polymorphisms in Type 2 Diabetes among Saudi Population
DOI:
https://doi.org/10.3889/oamjms.2022.10625Keywords:
Vitamin D, Type 2 diabetes mellitus, Vitamin D receptor, Gene polymorphism, ApaI, TaqIAbstract
Background: Variants of vitamin D receptor (VDR) gene have been linked to a variety of diseases, including metabolic syndrome, cancer, bone disease, and tuberculosis. The relationship between VDR gene mutations and the susceptibility of type 2 diabetes mellitus (T2DM) in different ethnic groups is yet unknown. Vitamin D and its receptor complex have a function in regulating β-cell insulin secretion as a transcription factor.
Objectives: The goal of this study was to see if there is a link between VDR Apa1 and Taq1 polymorphisms and T2DM susceptibility in the Saudis of Makkah environ.
Materials and Methods: DNA was separated from peripheral blood and genotyped in 110 healthy controls and 110 unrelated people with T2DM for the VDR ApaI (G/T) rs7975232 and TaqI (A/G) rs731236 single nucleotide polymorphisms (SNPs) using polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) technique.
Results: The distributions of genotypes and alleles of VDR ApaI and TaqI polymorphisms were statistically indifferent across the groups investigated (P >0.05).
Conclusion: These findings showed that polymorphisms in the VDR ApaI and TaqI genes may not be linked to T2DM risk in Saudis.
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Copyright (c) 2022 Fathy M. Elfasakhany, Mashael S. Alqahtani, Ayman M. A. Elguindy, Magdi A. El-Damarawi (Author)
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