Investigation on the Expectorant Effect of Extracts from Primula veris L.

Authors

  • Svitlana Marchyshyn Department of Pharmacognosy and Medical Botany, I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
  • Liudmyla Slobodianiuk Department of Pharmacognosy and Medical Botany, I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
  • Liliia Budniak Department of Pharmacy Management
  • Liubov Shostak Department of Pharmacy, Drug Technology and Pharmaceutical Management, Kyiv International University, Kyiv, Ukraine
  • Oleg Gerush Department of Pharmacy, Bukovinian State Medical University, Chernivtsi, Ukraine

DOI:

https://doi.org/10.3889/oamjms.2022.10657

Keywords:

Primula veris L., Cowslip primrose, Thick extract, Leaves, Rhizomes with roots, Expectorant effect

Abstract

BACKGROUND: At present, coughing can be regulated by medications such as dextromethorphan and codeine, which are associated with side effects, including drug drowsiness or dependency. Thus, there is an increasing demand for drugs that promote expectorant activities with fewer adverse effects. The root of Primula veris L. (Primulaceae) is an herbal medicine that has been used as an expectorant drug for thousands of years in folk medicine.

AIM: The present study aims to create an in-depth pharmacological study of the expectorant activity of P. veris to create new drugs in different directions which are appropriate and promising.

MATERIALS AND METHODS: The expectorant effect of thick extracts of the study plant was studied on the influence of the motor activity of the ciliated epithelium and the secretory function of the bronchi. The expectorant activities of the ethanol extracts of leaves and rhizomes with roots from P. veris were evaluated using classical animal models. The expectorant assay was performed with phenol red secretion in the mouse trachea. After gastric administration of the test extracts in mice, 2.5% phenol red solution was injected intraperitoneally. The trachea was dissected and the optical density of tracheal secretion was measured.

RESULTS: The results of the studies showed that a thick extract of primrose rhizomes with roots has a high ability to secrete sputum, which is almost not inferior to the Hedelix drops comparator (ivy extract) – 126.6% and 146.4%, respectively. Extract from the leaves of P. veris is characterized by less pronounced activity, which, at a dose of 200 mg/kg, was 74.5%.

CONCLUSION: The results of the present study provide evidence that P. veris can be used as an expectorant herbal medicine and that triterpene saponins may be the main active ingredients of Primula veris responsible for its bioactivities.

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References

Latypova GM, Bychenkova MA, Katayev VA, Perfilova VN, Tyurenkov I, Mokrousov IS, et al. Composition and cardioprotective effects of solid herbal extract of Primula veris L. solid herbal extract in experimental chronic heart failure. Phytomedicine. 2019;54:17-26. https://doi.org/10.1016/j.phymed.2018.09.015 PMid:30668367 DOI: https://doi.org/10.1016/j.phymed.2018.09.015

Fico G, Rodoni G, Flamini G, Passarella D, Tome F. Comparative phytochemical and morphological analyses of three Italian Primula species. Phytochemistry. 2007;68(12):1683-91. https://doi.org/10.1016/j.phytochem.2007.04.019. PMid:17544462 DOI: https://doi.org/10.1016/j.phytochem.2007.04.019

Colombo PS, Flamini G, Rodondi G, Giuliani C, Santagostini L, Fico G. Phytochemistry of European Primula species. Phytochemistry. 2017;143:132-44. https://doi.org/10.1016/j.phytochem.2017.07.005 PMid:28806605 DOI: https://doi.org/10.1016/j.phytochem.2017.07.005

Sinichenko A, Shostak L, Marchyshyn S, Kozachok S. Determination of flavonoids in flowers of herbs from Primula genus by HPLC method. In: Materials of 10th International Symposium on Chromatography of Natural Products. Poland: Lublin; 2016. p. 165.

Bączek K, Przybył JL, Mirgos M, Kosakowska O, Szymborska- Sandhu I, Węglarz Z. Phenolics in Primula veris L. and P. elatior (L.) Hill Raw Materials. Int J Anal Chem. 2017;2017:2871579. https://doi.org/10.1155/2017/2871579 DOI: https://doi.org/10.1155/2017/2871579

Apel L, Kammerer DR, Stintzing FC, Spring O. Comparative metabolite profiling of triterpenoid saponins and flavonoids in flower color mutations of Primula veris L. Int J Mol Sci. 2017;18(1):153. https://doi.org/10.3390/ijms18010153. PMid:28098796 DOI: https://doi.org/10.3390/ijms18010153

Huck CW, Huber CG, Ongania KH, Bonn GK. Isolation and characterization of metoxylated flavones in the flowers Primula veris by liquid chromatography and mass spectrometry. J Chromatogr A. 2000;870(1-2):453-62. https://doi.org/10.1016/s0021-9673(99)00950-4 PMid:10722102 DOI: https://doi.org/10.1016/S0021-9673(99)00950-4

Kirnichyshyn OR. Chemical compositions of Primula veris L. Sci Bull NLTU Ukr 2012;22(14):59-64.

Müller A, Ganzera M, Stuppner H. Analysis of phenolic glycosides and saponins in Primula elatior and Primula veris (Primula root) by liquid chromatography, evaporative light scattering detection and mass spectrometry. J Chromatogr A. 2006;1112(1-2):218-23. https://doi.org/10.1016/j.chroma.2005.10.067 PMid:16297921 DOI: https://doi.org/10.1016/j.chroma.2005.10.067

Al-Hussaniy HA. Methicillin-resistant Staphylococcus aureus and New Delhi metallo beta-lactamases: Types of antibiotic resistance, methods of prevention. Med Pharm J. 2022;1(1):1-18.

Al-hussaniy H, Al-tameemi ZS. Methicillin-resistant Staphylococcus aureus and New Delhi metallo beta-lactamases- types of antibiotic resistance, methods of prevention. Med Pharm J 2022;1(1):14-24. https://doi.org/10.55940/medphar20223 DOI: https://doi.org/10.55940/medphar20223

Başbülbül G, Özmen A, Biyik HH, Sen Ö. Antimitotic and antibacterial effects of the Primula veris L. flower extracts. Caryologia. 2008;61(1):88-91. DOI: https://doi.org/10.1080/00087114.2008.10589614

Qazi NS, Fiaz A, Mansoor A. Antimicrobial and cytotoxicity activities of the medicinal plant Primula macrophylla. J Enzyme Inhib Med Chem. 2009;24(3):697-701. https://doi.org/10.1080/14756360802333406 PMid:18686139 DOI: https://doi.org/10.1080/14756360802333406

Tokalov SV, Kynd B, Wollenweber E, Gutzeyt HE. Biological effects of epicuticular flavonoids from Primula denticulata on human. J Agric Food Chem. 2004;52(2):239-45. https://doi.org/10.1021/jf0347160 PMid:14733502 DOI: https://doi.org/10.1021/jf0347160

Latypova GM, Iksanova GR, Bychenkova MA, Ivanova DF. To the study of new pharmacological properties of plant extract and spring primrose grass. Tradit Med. 2014;4(39):14-6.

Rubin BK. Surface properties of respiratory secretions: Relationship to mucus transport. In: Baum G, editor. Cilia, Mucus, and Mucociliary Interactions. New York: Marcel Dekker; 1998. p. 317-24.

Song KJ, Shin YJ, Lee KR, Lee EJ, Suh YS, Kim KS. Expectorant and antitussive effect of hedera helix and rhizoma coptidis extracts mixture. Yonsei Med J. 2015;56(3):819-24. https://doi.org/10.3349/ymj.2015.56.3.819 PMid:25837191 DOI: https://doi.org/10.3349/ymj.2015.56.3.819

Al-hussaniy HA, Altalebi RR, Albu-Rghaif AH, Abdul-Amir AG. The use of PCR for respiratory virus detection on the diagnosis and treatment decision of respiratory tract infections in Iraq. J Pure Appl Microbiol. 2022;16(1):201-6. https://doi.org/10.22207/jpam.16.1.10 DOI: https://doi.org/10.22207/JPAM.16.1.10

Jiangsu New Medical College. Encyclopedia of Chinese Materia Medica (Appendix). Shanghai, China: Shanghai People’s Press; 1977.

Guo T, Wei JQ, Ma JP. Antitussive and expectorant activities of Potentilla anserine. Pharm Biol. 2016;54(5):807-11. http://doi.org/10.3109/13880209.2015.1080734 PMid:26695537 DOI: https://doi.org/10.3109/13880209.2015.1080734

Al-Hassany HA, Albu-Rghaif AH, Naji MA, Naji MA. Tumor diagnosis by genetic markers protein P-53, p16, C-MYC, N-MYC, protein K-Ras, and gene her-2 Neu is this possible? Pakistan J Med Health Sci. 2021;16(8):2350-4. DOI: https://doi.org/10.53350/pjmhs211582350

Feshchenko H, Marchyshyn S, Budniak L, Slobodianiuk L, Basaraba R. Study of antibacterial and antifungal properties of the lyophilized extract of fireweed (Chamaenerion angustifolium L.) herb. Pharmacologyonline. 2021;2:1464-72.

Budniak L, Slobodianiuk L, Marchyshyn S, Parashchuk E. Determination of carbohydrates in burnet saxifrage (Pimpinella saxifraga L.). Pharmacologyonline. 2021;2:1374-82.

Budniak L, Slobodianiuk L, Marchyshyn S, Ilashchuk P. Determination of polysaccharides in Gentiana cruciata L. herb. Pharmacologyonline. 2021;2:1473-79.

Tarapatskyy M, Gumienna A, Sowa P, Kapusta I, Puchalski C. Bioactive phenolic compounds from Primula veris L.: Influence of the extraction conditions and purification. Molecules. 2021;26(4):997. http://doi.org/10.3390/molecules26040997 PMid:33668513 DOI: https://doi.org/10.3390/molecules26040997

Marchyshyn S, Slobodianiuk L, Budniak L, Ivasiuk I. Hypoglycemic effect of Cyperus esculentus L. tubers extract. Pharmacologyonline. 2021;2:1383-92.

Engler H, Szelenyi I. Tracheal phenol red secretion, a new method for screening mucosecretolytic compounds. J Pharmacol Methods. 1984;11(3):151-7. http://doi.org/10.1016/0160-5402(84)90033-0 PMid:6738080 DOI: https://doi.org/10.1016/0160-5402(84)90033-0

Lin BQ, Li PB, Wang YG, Peng W, Wu Z, Su W, et al. The expectorant activity of naringenin. Pulm Pharmacol Ther. 2008;21(2):259-63. http://doi.org/10.1016/j.pupt.2007.05.001 PMid:17664077 DOI: https://doi.org/10.1016/j.pupt.2007.05.001

Sernov LN, Gatsura VV. Elements of Experimental Pharmacology. Moscow: Nauka; 2000. p. 7.

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Published

2022-08-15

How to Cite

1.
Marchyshyn S, Slobodianiuk L, Budniak L, Shostak L, Gerush O. Investigation on the Expectorant Effect of Extracts from Primula veris L. Open Access Maced J Med Sci [Internet]. 2022 Aug. 15 [cited 2024 Apr. 18];10(A):1368-72. Available from: https://oamjms.eu/index.php/mjms/article/view/10657

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