Screening for Anti Mycobacterium tuberculosis Activity of Streptomyces sp. from Lapindo Mud in Sidoarjo, Indonesia

Rochmah Kurnijasanti; Muhammad  Rais; Sri Agus  Sudjarwo

doi:10.3889/oamjms.2023.10765

Authors

Rochmah Kurnijasanti Department of Basic Veterinary Medicine, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia
Muhammad Rais Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia
Sri Agus Sudjarwo Department of Basic Veterinary Medicine, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2023.10765

Keywords:

Screening, Streptomyces, Anti-Mycobacterium tuberculosis, Lapindo mud, Indonesian women

Abstract

BACKGROUND: Streptomyces sp. from Indonesian soil have not been explored and isolated to find new strains as a source of antibiotics for the treatment of tuberculosis (TB) disease.

AIM: In this study, the effect of Streptomyces spp. from Lapindo mud in Sidoarjo, Indonesia be observed, to find out whether Streptomyces spp. has anti-TB activity.

METHODS: The primers Strep F; 5-AGAGTTTGAT CCTGKGTCAG-3 and Strep R; 5-AAGGGAG GTGATCCAKKGKGA-3 were used in polymerase chain reaction amplification of the 16S rRNA gene against Streptomyces strains. The anti-TB activity of Streptomyces sp. was determined by broth dilution method using Middlebrook 7H9 media.

RESULTS: The results showed that new types of Streptomyces spp., namely, Streptomyces A, Streptomyces D, Streptomyces Ea, Streptomyces Ep, Streptomyces I, Streptomyces F, and Streptomyces G from garbage dump soils. This result also showed that the activity of Streptomyces I, Streptomyces F, and Streptomyces G could inhibit the Mycobacterium TB growth by with inhibitory zones, respectively, 2 ± 0.3; 8 ± 0.7 and 15 ± 0.9mm, while Streptomyces A, Streptomyces D, Streptomyces Ea, and Streptomyces Ep did not inhibit M. TB.

CONCLUSION: Thus, from the results obtained, it can be concluded that Streptomyces extract mainly Streptomyces G has promising anti-TB activity by preliminary in vitro techniques. Therefore, it has the definite potential as a source of compounds that may be developed further into antimycobacterial drugs.

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