TGF-1 mRNA, AFP-L3, and Annexin II in the Early and Late Detection of Hepatocellular Carcinoma: The Diagnostic Value

Authors

  • Eman Youssef Department of Biochemistry, Faculty of Medicine, Al-Azhar University, Nasr, Cairo, Egypt; Department of Biochemistry, College of Medicine, Taif University, Taif, Saudi Arabia
  • Nashwa El-Khouly Department of Internal Medicine, Faculty of Medicine, Al-Azhar University, Nasr, Cairo, Egypt
  • Yahia Abdullah Elzahrani Department of Internal Medicine, College of Medicine, Taif University, Taif, Saudi Arabia
  • Rehab M. E. Tash Department of Medical Microbiology and Immunology, Faculty of Medicine, Zagazig University, Zagazig, Egypt
  • Eman A. Khalifa Department of Parasitology, Faculty of Medicine, Tanta University, Tanta, Egypt; Department of Parasitology, College of Medicine, Taif University, Taif, Saudi Arabia
  • Eman S. M. Bayoumy Department of Biochemistry, Faculty of Medicine, Al-Azhar University, Nasr, Cairo, Egypt; Department of Biochemistry, College of Medicine, Taif University, Taif, Saudi Arabia
  • Mona Khalil Department of Biochemistry, Faculty of Medicine, Al-Azhar University, Nasr, Cairo, Egypt https://orcid.org/0000-0002-4529-6381
  • Awatif E. Edreis Department of Tropical Medicine, Faculty of Medicine, Tanta University, Tanta, Egypt; Department of Internal Medicine, College of Medicine, Taif University, Taif, Saudi Arabia
  • Fatma Saffeyeldin Mohamed Department of Internal Medicine, College of Medicine, Taif University, Taif, Saudi Arabia; Department of Tropical Medicine, Faculty of Medicine, Al-Azhar University, Nasr, Cairo, Egypt
  • Aml E. Abdou Department of Microbiology and Immunology, Faculty of Medicine, Al-Azhar University, Nasr, Cairo, Egypt
  • Nora Seliem Department of Biochemistry, Faculty of Medicine, Al-Azhar University, Nasr, Cairo, Egypt https://orcid.org/0000-0001-9541-6479
  • Mahmoud Sofy Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr, Cairo, Egypt
  • Sara Fakhrelden Department of Tropical, Faculty of Medicine, Fayoum University, Fayoum, Egypt
  • Sohaila M. H. Marmoush Deparmtent of Biomedical Science, Faculty of Medical Sciences, Newcastle University Medicine Malaysia, Johor, Malaysia
  • Ghada F. Elmohaseb Department of Internal Medicine, Faculty of Medicine, Al-Azhar University, Nasr, Cairo, Egypt
  • Amal A. Elhosary Deparmtent of Clinical Pathology, Faculty of Medicine, Menoufia University, Shebin ElKoum, Egypt

DOI:

https://doi.org/10.3889/oamjms.2022.10814

Keywords:

HCC, tumor markers, AFP-L3, Annexin II, TGF-β1 gene expression

Abstract

BACKGROUND: Alpha-fetoprotein (AFP) is the recommended screening biomarker for hepatocellular carcinoma (HCC), despite its drawbacks: AFP-negative HCC, poor specificity, and sensitivity. As a result, new HCC-sensitive and specific biomarkers are urgently needed.

AIM: This study aimed to determine the diagnostic value of transforming growth factor (TGF)-β1 mRNA and Annexin II in the early detection and follow-up of HCC.

PATIENT AND METHODS: This research involved 75 HCC patients (30 early and 45 late) and 75 liver cirrhosis (LC) patients (all patients have HCV), and 75 healthy individuals as controls. Reverse transcription polymerase chain reaction measured TGF-β1 mRNA. Enzyme-linked immunosorbent assay ELISA measured Annexin II, AFP-L3, and AFP.

RESULTS: Annexin II was a biomarker with a significant difference between the LC and early HCC groups. TGF-β1 mRNA showed a significant difference when the LC group was compared to the control group and the late HCC group.

CONCLUSION: Annexin II has better sensitivity and specificity for early HCC detection than AFP, and TGF-β1 mRNA can be used for the assessment of the degree of HCC, and TGF-1 signaling inhibitors may be a possible new treatment choice for HCC.

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Published

2022-12-28

How to Cite

1.
Youssef E, El-Khouly N, Elzahrani YA, Tash RME, Khalifa EA, Bayoumy ESM, Khalil M, Edreis AE, Mohamed FS, Abdou AE, Seliem N, Sofy M, Fakhrelden S, Marmoush SMH, Elmohaseb GF, Elhosary AA. TGF-1 mRNA, AFP-L3, and Annexin II in the Early and Late Detection of Hepatocellular Carcinoma: The Diagnostic Value. Open Access Maced J Med Sci [Internet]. 2022 Dec. 28 [cited 2024 Mar. 28];10(A):15-22. Available from: https://oamjms.eu/index.php/mjms/article/view/10814