Association between the HLA-DQB1 Gene Polymorphism and Chronic Progression of Hepatitis B in Indonesia

Fatmawati Fatmawati; Ellyza Nasrul; Nasrul  Zubir; Jamsari Jamsari; Bastian  Nova; Aulia Janer

doi:10.3889/oamjms.2022.10818

Authors

Fatmawati Fatmawati Department of Biomedical Science, Faculty of Medicine, Andalas University, Padang, West Sumatra, Indonesia
Ellyza Nasrul Department of Clinical Pathology, Faculty of Medicine, Andalas University, Limau Manis, Padang, West Sumatra, Indonesia
Nasrul Zubir Department of Internal Medicine, Faculty of Medicine, Andalas University, Limau Manis, Padang, West Sumatra, Indonesia
Jamsari Jamsari Department of Agrotechnology, Faculty of Agriculture, Andalas University, Limau Manis, Padang, West Sumatra, Indonesia
Bastian Nova Department of Agrotechnology, Faculty of Agriculture, Andalas University, Limau Manis, Padang, West Sumatra, Indonesia
Aulia Janer Department of Surgery, Faculty of Medicine, Riau University, Pekanbaru, Riau, Indonesia https://orcid.org/0000-0002-9432-8589

DOI:

https://doi.org/10.3889/oamjms.2022.10818

Keywords:

HLA-DQB1, Chronic hepatitis B, HLA Polymorphism, Hepatitis B progression

Abstract

BACKGROUND: The progression of hepatitis B is affected by the activity of T lymphocytes. Activation of T lymphocytes requires a primary signal originating from the presentation of antigen by HLA molecules to T cell receptors. HLA-DQB1 gene polymorphisms can affect the ability of HLA to bind and present viral antigens to T cells, thus affecting T cell activation and potentially associated with the progression of chronic hepatitis B.

AIM: We aimed to investigate the polymorphisms of the HLA-DQB1 gene and its influence on chronic hepatitis B progression of chronic hepatitis B patients in Indonesia.

METHODS: This cross-sectional research studied chronic hepatitis B patients at the Internal Medicine Department, Arifin Ahmad Hospital, Pekanbaru, from January 2018 to December 2018. Subjects were grouped into three categories: (1) Inactive chronic hepatitis B, (2) active chronic hepatitis B, and (3) end stage liver disease (ESLD) which consisted of patients with liver cirrhosis and hepatocellular carcinoma. Examination of the HLA-DQB1 gene polymorphism was performed with the SSP-PCR method and sequenced to verify the PCR results. Analysis results of p < 0.05 were considered statistically significant.

RESULTS: The most common allele in patients with chronic hepatitis B was the HLA-DQB1 0301. The HLA-DQB1 0301 allele found primarily in the inactive chronic hepatitis B group. The DQB1 0501 allele found to be more abundant in patients with active chronic hepatitis B. The HLA DQB1 0502 allele only found in patients with chronic hepatitis B with ESLD.

CONCLUSIONS: The HLA-DQB1 gene polymorphism is associated with the progression of chronic hepatitis B in chronic hepatitis B patients in Indonesia.

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