The Antifungal and Antibacterial Effect of Citrullus Colocynthis: In Vitro Study


  • Elahe Tahmasebi Research Center for Prevention of Oral and Dental Diseases, Baqiyatallah University of Medical Sciences, Tehran, Iran
  • Mohsen Yazdanian Research Center for Prevention of Oral and Dental Diseases, Baqiyatallah University of Medical Sciences, Tehran, Iran
  • Hamid Tebyaniyan Department of Science and Research, Islamic Azad University, Tehran, Iran
  • Armin Soudi Department of Orthodontics, Faculty of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
  • Reza Ranjbar Research Center for Prevention of Oral and Dental Diseases, Baqiyatallah University of Medical Sciences, Tehran, Iran
  • Mehrdad Moosazadeh Moghaddam Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
  • Majid Balaei-Kahnamoei Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  • Somayeh Khoramian Tusi Dental College, Alborz University of Medical Sciences, Karaj, Iran
  • Mahsima Tayefi Nasrabadi Dental College, Alborz University of Medical Sciences, Karaj, Iran
  • Mohammad Taghi Vatandoust Department of Orthodontics, School of Dentistry, Shahed University of Medical Sciences, Tehran, Iran



Alcoholic extract, Antibacterial, Antifungal, Citrullus colocynthis


Introduction: Bacteria almost exclusively cause caries and other oral and dental diseases. This study evaluated the inhibitory effect of the alcoholic extract of Citrullus colocynthis on bacteria and fungi common in oral bacteria diseases.

Method and materials: The ethanol extract and essential oil of Citrullus colocynthis were obtained via water distillation. After conducting phytochemical tests, including determination of total phenolic and flavonoid compounds of the extracts, GC-MASS test and microbial tests of the extracts were conducted on S. mutans, E. coli, S. salivarius, L. acidophilus, C. albicans, and S. aureus. The cytotoxicity test was assessed by MTT assay.

Results: MIC and MBC values for S. mutans, S. salivalius, and L. acidophilus were 1.56 mg/ml and 3.12 mg/ml, respectively. Moreover, Citrullus colocynthis also has an inhibitory effect on bacteria and C. albicans at these concentrations, according to the DAD test. The survival rate of cells at concentrations of 5 mg/ml and 10 mg/ml was less than 50%, and at concentrations of 2.5 mg/ml and 1.25 mg/ml was less than 70% after 24 hours and 48 hours.

Conclusion: According to the cytotoxicity results of this plant at concentrations of 1.56 mg/ml and 3.125 mg/ml, the ethanolic extract of C. colocynthis has inhibitory and lethal effects against pathogenic bacteria and fungi.


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How to Cite

Tahmasebi E, Yazdanian M, Tebyaniyan H, Soudi A, Ranjbar R, Moghaddam MM, Balaei-Kahnamoei M, Tusi SK, Nasrabadi MT, Vatandoust MT. The Antifungal and Antibacterial Effect of Citrullus Colocynthis: In Vitro Study. Open Access Maced J Med Sci [Internet]. 2022 Oct. 14 [cited 2024 Jul. 24];10(A):1585-91. Available from:

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