The Comparison of Normoxic and Hypoxic Mesenchymal Stem Cells in Regulating Platelet-derived Growth Factors and Collagen Serial Levels in Skin Excision Animal Models

Erni Daryanti; Agung Putra; Titik Sumarawati; Nur Dina Amalina; Ardi Prasetio; Husni Ahmad Sidiq

doi:10.3889/oamjms.2023.10966

Authors

Erni Daryanti Department of Biomedical Science, Medical Faculty, Sultan Agung Islamic University (UNISSULA), Semarang, Indonesia
Agung Putra Stem Cell and Cancer Research (SCCR), Medical Faculty, Sultan Agung Islamic University (UNISSULA), Semarang, Indonesia; Department of Pathology, Medical Faculty, Sultan Agung Islamic University (UNISSULA), Semarang, Indonesia; Department of Postgraduate Biomedical Science, Medical Faculty, Sultan Agung Islamic University (UNISSULA), Semarang, Indonesia https://orcid.org/0000-0003-4261-9437
Titik Sumarawati Department of Postgraduate Biomedical Science, Medical Faculty, Sultan Agung Islamic University (UNISSULA), Semarang, Indonesia
Nur Dina Amalina Stem Cell and Cancer Research (SCCR), Medical Faculty, Sultan Agung Islamic University (UNISSULA), Semarang, Indonesia; Pharmacy Study Program, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang, Semarang, Indonesia
Ardi Prasetio Stem Cell and Cancer Research (SCCR), Medical Faculty, Sultan Agung Islamic University (UNISSULA), Semarang, Indonesia
Husni Ahmad Sidiq Stem Cell and Cancer Research (SCCR), Medical Faculty, Sultan Agung Islamic University (UNISSULA), Semarang, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2023.10966

Keywords:

Collagen, Hypoxia, Mesenchymal stem cells, Platelet-derived growth factors, Skin healing

Abstract

BACKGROUND: The healing process of a skin excisions involves a complex cascade of cellular responses to reverse skin integrity formation. These processes require growth factors particularly platelet-derived growth factors (PDGF). On the other hand, hypoxia- preconditioned mesenchymal stem cells (MSCs) could secrete growth factors that notably contribute to wound healing acceleration, characterized by the enhancement of collagen density.

AIM: This study was aimed to investigate the role of hypoxia-preconditioned MSCs in regulating the serial levels of PDGF associated with the enhancement of collagen density in the skin excision animal models.

METHODS: Twenty-seven male Wistar rats of skin excision were created as animal models. The animals were randomly assigned into four groups consisting of two treatment groups (treated by normoxia-preconditioned MSCs as T1 and hypoxia-preconditioned MSCs as T2), positive control (treated with phosphate-buffered saline) and sham (non-treated and healthy rats). PDGF levels were examined by ELISA. The collagen density was determined using Masson‘s trichrome staining.

RESULTS: This study showed that there was a significant increase in PDGF levels on days 3 and 6 after hypoxia- preconditioned MSCs treatment. In line with these findings, the collagen density was also increased significantly after hypoxia-preconditioned MSCs treatment on days 3, 6, and 9.

CONCLUSION: Hypoxia-preconditioned MSCs could regulate the serial PDGF levels that lead to the enhancement of collagen density in the skin excision rat’s model.

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