Correlation of Delayed Onset Muscle Soreness and Inflammation Post-exercise Induced Muscle Damage


  • Soni Sulistyarto Department of Sport Science, Faculty of Sport Science, Universitas Negeri Surabaya, Surabaya 60123, East Java, Indonesia
  • Roy Irawan Department of Sport Science
  • Noortje Anita Kumaat Department of Sport Science
  • Nanda Rimawati Department of Sport Science, Faculty of Sport Science, Universitas Negeri Surabaya, Surabaya 60123, East Java, Indonesia



Delayed onset muscle soreness, High mobility group box 1, Eccentric-exercise, Muscle-damage, Inflammation


BACKGROUND: Delayed Onset Muscle Soreness (DOMS) is a common injury resulting from abnormal intensive training in athletes, mainly the result of training involving eccentric contractions.

AIM: The aim of this study was to determine the correlation between the incidence of DOMS and HMGB1 as a marker of the occurrence of inflammation post Exercise Induces Muscle Damaged.

METHOD: 28 male recreational students of Sports Science Department, Universitas Negeri Surabaya who met all inclusion and exclusion criteria participated in this study. Participants completed a muscle damaging exercise which consists of a 10x10 drop jump (DRP) and a bout of 40×15 m sprints with a 5 m deceleration zone (SPR) to obtain a muscle damage effect. In this study, the stretching in the exercise session was not given, this was done to get the DOMS effect after exercise. DOMS and HMGB1 was carry out 1 hour before the exercise, 12 hours after the exercise, 24 hours after the exercise and 48 hours after the exercise.

RESULT: The result showed that there was a significant correlation (r=0.935, p<0.05) between DOMS and HMGB1 as a predictor of inflammation. The participants that were given EIMD eccentric exercise (DRP and SPR) showed the occurrence of DOMS and increasing of HMGB1. The result also showed that there was a correlation between DOMS and HMGB1.

CONCLUSION: This study concluded that there was a correlation between DOMS and HMGB1 as a marker of inflammation as the result of the eccentric exercise of the exercise Induced Muscle Damage (EIMD).


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Diep S. DOMS. Ultrasound Med Biol. 2019;45:S46. DOI:

Fleckenstein J, Neuberger EW, Bormuth P, Comes F, Schneider A, Banzer W, et al. Investigation of the sympathetic regulation in delayed onset muscle soreness: Results of an RCT. Front Physiol. 2021;12:1-13. DOI:

Han K, Kwon O, Jung SY, Park IH, Hwang MS, Park SY, et al. Jakyakgamcho-tang in the relief of delayed-onset muscle soreness in healthy adults: Study protocol for a randomized, double-blind, placebo-controlled, crossover design clinical trial. Trials. 2020;21(1):211. PMid:32085792 DOI:

Nobari H, Aquino R, Clemente FM, Khalafi M, Adsuar JC, Gómez JP. Description of acute and chronic load, training monotony and strain over a season and its relationships with well-being status: A study in elite under-16 soccer players. Physiol Behav. 2020;15:225. PMid:32750432 DOI:

Kaczmarek A, Kaczmarek M, Ciałowicz M, Clemente FM, Wolański P, Badicu G, et al. The role of satellite cells in skeletal muscle regeneration-the effect of exercise and age. Biology (Basel). 2021;10(10):1056. PMid:34681155 DOI:

McHugh MP, Tyler TF. Muscle strain injury vs muscle damage: Two mutually exclusive clinical entities. Transl Sport Med. 2019;2:102-8. DOI:

Hotfiel T, Freiwald J, Hoppe MW, Lutter C, Forst R, Grim C, et al. Advances in delayed-onset muscle soreness (DOMS): Part I: Pathogenesis and diagnostics. Sportverletz Sportschaden. 2018;32(4):243-50. PMid:30537791 DOI:

Bontemps B, Vercruyssen F, Gruet M, Louis J. Downhill running: What are the effects and how can we adapt? A narrative review. Sport Med. 2020;50(12):2083-110. PMid:33037592 DOI:

Pincheira PA, Hoffman BW, Cresswell AG, Carroll TJ, Brown NA, Lichtwark GA. Cyclic eccentric stretching induces more damage and improved subsequent protection than stretched isometric contractionsin the lower limb. Eur J Appl Physiol. 2021;121(12):3349-60. PMid:34436674 DOI:

Shen L, Meng X, Zhang Z, Wang T. Physical exercise for muscle atrophy. Adv Exp Med Biol. 2018;1088:529-45. PMid:30390268 DOI:

Hayashi K, Katanosaka K, Abe M, Yamanaka A, Nosaka K, Mizumura K, et al. Muscular mechanical hyperalgesia after lengthening contractions in rats depends on stretch velocity and range of motion. Eur J Pain. 2017;21(1):125-39. PMid:27351999 DOI:

Scheffer DL, Latini A. Exercise-induced immune system response: Anti-inflammatory status on peripheral and central organs. Biochim Biophys Acta Mol Basis Dis. 2020;1866(10):165823. PMid:32360589 DOI:

Choe JY, Choi CH, Park KY, Kim SK. High-mobility group box 1 is responsible for monosodium urate crystal-induced inflammation in human U937 macrophages. Biochem Biophys Res Commun. 2018;503(4):3248-55. PMid:30166062 DOI:

Yang H, Wang H, Andersson U. Targeting inflammation driven by HMGB1. Front Immunol. 2020;11:484. PMid:32265930 DOI:

Ottestad W, Rognes IN, Skaga E, Frisvoll C, Haraldsen G, Ekenet T, et al. HMGB1 concentration measurements in trauma patients: Assessment of pre-analytical conditions and sample material. Mol Med. 2020;26(1):5. PMid:31892315 DOI:

Lau WY, Muthalib M, Nosaka K. Visual analog scale and pressure pain threshold for delayed onset muscle soreness assessment. J Musculoskelet Pain. 2013;21:320-6. DOI:

Gulick DT, Kimura IF, Sitler M, Paolone A, Kelly JD. Various treatment techniques on signs and symptoms of delayed onset muscle soreness. J Athl Train. 1996;31(2):145-52. PMid:16558388

Langley GB, Sheppeard H. The visual analogue scale: Its use in pain measurement. Rheumatol Int. 1985;5(4):145-8. PMid:4048757 DOI:

Tokinoya K, Ishikura K, Ra S, Ebina K, Miyakawa S, Ohmori H. Relationship between early-onset muscle soreness and indirect muscle damage markers and their dynamics after a full marathon. J Exerc Sci Fit. 2020;18(3):115-21. PMid:32351588 DOI:

Suzuki K, Tominaga T, Ruhee RT, Ma S, Characterization and modulation of systemic inflammatory response to exhaustive exercise in relation to oxidative stress. Antioxidants (Basel). 2020;9(5):401. PMid:32397304 DOI:

Fernández-Lázaro D, Mielgo-Ayuso J, Calvo JS, Martínez AC, García AC, Fernandez-Lazaro C. Modulation of exerciseinduced muscle damage, inflammation, and oxidative markers by curcumin supplementation in a physically active population: A systematic review. Nutrients. 2020;12(2):501. PMid:32075287 DOI:

Irawan R, Mahmudiono T, Martiana T. Interleukin-6 as immune system and inflammation biomarker on the response of basic pencak silat exercise in perguruan pencak silat perisai diri, bojonegoro. Open Access Maced J Med Sci. 2021;9:179-83. DOI:

Chen HY, Chen YC, Tung K, Chao HH, Wang HS. Effects of caffeine and sex on muscle performance and delayedonset muscle soreness after exercise-induced muscle damage: A double-blind randomized trial. J Appl Physiol (1985). 2019;127(3):798-805. PMid:31219772 DOI:

Kawamura T, Suzuki K, Takahashi M, Tomari M, Hara R, Gando Y, et al. Involvement of neutrophil dynamics and function in exercise-induced muscle damage and delayed-onset muscle soreness: Effect of hydrogen bath. Antioxidants (Basel). 2018;7(10):127. httpss:// PMid:30257503 DOI:

Massaro M, Scoditti E, Carluccio MA, Kaltsatou A, Cicchella A. Effect of cocoa products and its polyphenolic constituents on exercise performance and exercise-induced muscle damage and inflammation: A review of clinical trials. Nutrients. 2019;11(7):1471. PMid:31261645 DOI:

Akehurst H, Grice JE, Angioi M, Morrissey D, Migliorini F, Maffulli N. Whole-body vibration decreases delayed onset muscle soreness following eccentric exercise in elite hockey players: A randomised controlled trial. J Orthop Surg Res. 2021;16(1):589. PMid:34641941 DOI:

Sonkodi B. Delayed onset muscle soreness (Doms): The repeated bout effect and chemotherapy-induced axonopathy may help explain the dying-back mechanism in amyotrophic lateral sclerosis and other neurodegenerative diseases. Brain Sci. 2021;11(1):108. PMid:33467407 DOI:

Bongiovanni T, Genovesi F, Nemmer M, Carling C, Alberti G, Howatson G. Nutritional interventions for reducing the signs and symptoms of exercise-induced muscle damage and accelerate recovery in athletes: Current knowledge, practical application and future perspectives. Eur J Appl Physiol. 2020;120(9):1965-96. PMid:32661771 DOI:

Toumi H, Best TM. The inflammatory response: Friend or enemy for muscle injury? Br J Sports Med. 2003;37(4):284-6. PMid:12893707 DOI:

Hyldahl RD, Hubal MJ. Lengthening our perspective: Morphological, cellular, and molecular responses to eccentric exercise. Muscle Nerve. 2014;49(2):155-70. PMid:24030935 DOI:

Chazaud B. Inflammation during skeletal muscle regeneration and tissue remodeling: Application to exercise-induced muscle damage management. Immunol Cell Biol. 2016;94(2):140-5. https// PMid:26526620 DOI:

Tidball JG, Villalta SA. Regulatory interactions between muscle and the immune system during muscle regeneration. Am J Physiol Regul Integr Comp Physiol. 2010;298(5):R1173-87. PMid:20219869 DOI:

Abaïdia AE, Delecroix B, Leduc C, Lamblin J, McCall A, Baquet G, et al. Effects of a strength training session after an exercise inducing muscle damage on recovery kinetics. J Strength Cond Res. 2017;31(1):115-25. https// PMid:27227793 DOI:

Raucci A, Maggio SD, Scavello F, Ambrosio AD, Bianchi ME,Capogrossi MC. The Janus face of HMGB1 in heart disease: A necessary update. Cell Mol Life Sci. 2019;76(2):211-29. PMid:30306212 DOI:

Patel V, Dial K, Wu J, Gauthier AG, Wu W, Lin M, et al. Dietary antioxidants significantly attenuate hyperoxia-induced acute inflammatory lung injury by enhancing macrophage function via reducing the accumulation of airway HMGB1. Int J Mol Sci. 2020;21(3):977. PMid:32024151 DOI:

Alsousi AA, Igwe OJ. Redox-active trace metal-induced release of high mobility group box 1(HMGB1) and inflammatory cytokines in fibroblast-like synovial cells is Toll-like receptor 4 (TLR4) dependent. Biochim Biophys Acta Mol Basis Dis. 2018;1864(11):3847-58. PMid:30254017 DOI:




How to Cite

Sulistyarto S, Irawan R, Kumaat NA, Rimawati N. Correlation of Delayed Onset Muscle Soreness and Inflammation Post-exercise Induced Muscle Damage. Open Access Maced J Med Sci [Internet]. 2022 Nov. 4 [cited 2023 Mar. 22];10(A):1688-94. Available from:



Sports Medicine