Effect of Pakkat (Calamus caesius blume) Ethanol Extract on Testis Tissues Histology of Diabetic Rats

Yolanda Eliza Putri Lubis; Nyoman Lister; Bungaran Sihombing

doi:10.3889/oamjms.2023.10994

Authors

Yolanda Eliza Putri Lubis Department of Pharmacology, Faculty of Medicine, Universitas Prima Indonesia, Medan, North Sumatera, Indonesia
Nyoman Lister Department of Pharmacology, Faculty of Medicine, Universitas Prima Indonesia, Medan, North Sumatera, Indonesia
Bungaran Sihombing Department of Pharmacology, Faculty of Medicine, Universitas Prima Indonesia, Medan, North Sumatera, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2023.10994

Keywords:

Diabetes mellitus, Testis, Tissue, Pakkat (Calamus caesius Blume)

Abstract

Background: Diabetes Mellitus (DM) is a metabolic disease characterized by elevated blood sugar levels or chronic hyperglycemia. Diabetes has been shown to have an adverse effect on male and female reproductive function and its impact can be seen in the increasing prevalence of infertility. Pakkat is believed to be used as a medicine for diabetes mellitus. The chemical compounds contained in pakkat consist of flavonoids, alkaloids, terpenoids/steroids, saponins, tannins and glycosides which can be useful for lowering blood sugar levels.

Aim: To investigate the effectiveness of pakkat extract on Sperm Count and Motility Sperm of Male Wistar Rats with Diabetes Mellitus.

Methods: This study used a laboratory experimental design (true experiment) with a post test control group design only approach. The samples in this study were 25 diabetic rats that is grouped in 5 groups: control (-) group (NaCMC), control (+) group (metformin), Treatment Group I (extract dose of 125 mg/kgBW), Treatment Group II (extract dose of 250 mg/kgBW), Treatment Group III (extract at a dose of 500 mg/kgBW) given for 2 weeks.

Results: The results obtained in this research show that extract of pakkat at doses of 125mg/kgBB and 250mg/kgBB after 2 weeks could increase the number and moltility of spermatozoa.

Conclusion: Overall, it can be concluded of pakkat extract could increase the number and motility of spermatozoa of Male Wistar Rats with Diabetes Mellitus.

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References

Zheng Y, Ley SH, Hu FB. Global aetiology and epidemiology of Type 2 diabetes mellitus and its complications. Nat Rev Endocrinol. 2018;14(2):88-98. https://doi.org/10.1038/nrendo.2017.151 PMid:29219149 DOI: https://doi.org/10.1038/nrendo.2017.151

Indonesia PE. Pengelolaan dan Pencegahan Diabetes Melitus Tipe 2 di Indonesia. Indonesia: Pb Perkeni; 2015.

Saeedi P, Petersohn I, Salpea P, Malanda B, Karuranga S, Unwin N, et al. Global and regional diabetes prevalence estimates for 2019 and projections for 2030 and 2045: Results from the international diabetes federation diabetes atlas, 9th edition. Diabetes Res Clin Pract. 2019;157:107843. https://doi.org/10.1016/j.diabres.2019.107843 PMid:31518657 DOI: https://doi.org/10.1016/j.diabres.2019.107843

Wahyuni TD. The Ankle Brachial Index (ABI) after diabetes leg exercises with type 2 diabetes mellitus. J Keperawatan. 2015;4(2):143-51.

Tripathy JP, Thakur JS, Jeet G, Chawla S, Jain S, Pal A, et al. Prevalence and risk factors of diabetes in a large community-based study in North India: Results from a STEPS survey in Punjab, India. Diabetol Metab Syndr. 2017;9(1):1-8. https://doi.org/10.1186/s13098-017-0207-3 PMid:28127405 DOI: https://doi.org/10.1186/s13098-017-0207-3

Widyasari N. Relationship of Respondent’s Characteristic with The Risk of Diabetes Mellitus and Dislipidemia at Tanah Kalikedinding. J Berk Epidemiol. 2017;5(1):130-41. DOI: https://doi.org/10.20473/jbe.V5I12017.130-141

Namayandeh SM, Karimi A, Fallahzadeh H, Rahmanian M, Bafghi SM, Soltani M, et al. The incidence rate of diabetes mellitus (Type II) and its related risk factors: A 10-year longitudinal study of Yazd Healthy Heart Cohort (YHHC), Iran. Diabetes Metab Syndr Clin Res Rev. 2019;13(2):1437-41. https://doi.org/10.1016/j.dsx.2019.02.012 PMid:31336503 DOI: https://doi.org/10.1016/j.dsx.2019.02.012

Surbakti JM. Skrining Fitokimia dan Analisis Karbohidrat Secara Spektrofotometri Sinar Tampak pada Pakkat (Calamus caesius Blume.). Indonesia: Universitas Sumatera Utara; 2016.

Luo F, Li QC, Zhang FJ, Li L, Song LG, Mao Y, et al. Protective effect of adropin against high fat diet-induced obese diabetic wistar rats via nuclear factor erythroid 2-related factor 2 pathway. Pharmacogn Mag. 2020;16(69):250.

Yusuf N. Pemberian Ekstrak Daun Sukun (Artocarpus altilis) dalam Menurunkan Tekanan Darah pada Tikus Wistar Jantan (Rattus norvegicus) yang Diinduksi Prednison dan NaCl. Indonesia: Universitas Medan Area; 2017.

Abima F, Bahar M, Chairani A. Extract antibacterial effectiveness test binahong leaves (Anredera cordifolia (Ten.) Steenis) against Escherichia coli bacterial isolates from cilok snacks in vitro by the diffusion method. J Profesi Med J Kedokt dan Kesehat. 2017;11(1): 1-6. https://doi.org/10.33533/jpm.v11i1.205 DOI: https://doi.org/10.33533/jpm.v11i1.205

Afifah UN. Uji Aktivitas Antidiabetes Ekstrak Etanol 96% Buah Pare (Momordica charantia L.) Terhadap Tikus Jantan Galur Wistar Yang Diinduksi Aloksan. Indonesia: Universitas Muhammadiyah Surakarta; 2017.

Ibrahim AA, Abdussalami MS, Appah J, Umar AH, Ibrahim AA, Dauda KD. Antidiabetic effect of aqueous stem bark extract of Parinari macrophylla in alloxan-induced diabetic Wistar rats. Future J Pharm Sci. 2021;7(1):164. https://doi.org/10.1186/s43094-021-00303-6 DOI: https://doi.org/10.1186/s43094-021-00303-6

Chudasama RG, Dhanani NJ, Amrutiya RM, Chandni R, Jayanthi G, Karthikeyan K. Screening of selected plants from semi-arid region for its phytochemical constituents and antimicrobial activity. J Pharmacog Phytochem. 2018;7(2):2983-8.

Cobra LS, Amini HW. Phytochemical screening of turmeric (Curcuma longa) rhizome soxhletasi extract with 96% ethanol solvent. J Ilm Kesehat Karya Putra Bangsa. 2019;1(1):12-7.

Solikhah TI, Setiawan B, Ismukada DR. Antidiabetic activity of papaya leaf extract (Carica Papaya L.) isolated with maceration method in alloxan-induces diabetic mice. Syst Rev Pharm. 2020;11(9):774-8.

Mardina V, Harmawan T, Ilyas S, Tanjung M, Aulya W, Nasution A. Preliminary Phytochemical Screening of Different Solvent Extracts of Flower and Whole Plant of Wedelia biflora. In: IOP Conference Series: Materials Science and Engineering. United Kingdom: IOP Publishing; 2020. p. 12077. DOI: https://doi.org/10.1088/1757-899X/725/1/012077

Manzo LM, Moussa I, Ikhiri K. Phytochemical screening of selected medicinal plants used against diarrhea in Niger, West Africa. Int J Herb Med. 2017;5(4):32-8. DOI: https://doi.org/10.15171/ijep.2017.13

Sari SA, Ernita M, Mara MN, Rudi AR. Identification of active compounds on Muntingia calabura L. Leaves using different polarity solvents. Indones J Chem Sci Technol. 2020;3(1):1-7. DOI: https://doi.org/10.24114/ijcst.v3i1.18309

Sabina E, Zaidul IS, Ghafoor K, Jaffri JM, Sahena F, Babiker EE, et al. Screening of various parts of phaleria macrocarpa plant for α-glucosidase inhibitory activity. J Food Biochem. 2016;40(2):201-10. https://doi.org/10.1111/jfbc.12212 DOI: https://doi.org/10.1111/jfbc.12212

Mongi R, Simbala HE, de Queljoe E. Activity test for decreasing blood glucose levels of ethanol extract of areca yaki leaves (Areca vestiaria) against alloxan-induced male white rats (Rattus norvegicus). Pharmacon. 2019;8(2):449-56. https://doi.org/10.35799/pha.8.2019.29312 DOI: https://doi.org/10.35799/pha.8.2019.29312

Bari MW, Islam MM, Khatun M, Sultana MJ, Ahmed R, Islam A, et al. Antidiabetic effect of Wedelia chinensis leaf extract in alloxan induced Swiss albino diabetic mice. Clin Phytosci. 2020;6(1):1-8. https://doi.org/10.1186/s40816-020-00197-6 DOI: https://doi.org/10.1186/s40816-020-00197-6

Setianingsih H, Wisnumarta SE, Tjandra SA. Repairing of renal tubules in diabetic rats (Rattus norvegicus) diabetes after administration of golden sea cucumber (Stichopus hermanii). Pharmacogn J. 2021;13(4):958-64. https://doi.org/10.5530/pj.2021.13.123 DOI: https://doi.org/10.5530/pj.2021.13.123

Putra AM, Aulia D, Wahyuni A. Activity test for reducing blood sugar levels of ethanol extract of areca yaki leaves (Areca vestiaria) against alloxan-induced male white rats (Rattus norvegicus). J Ilm Ibnu Sina. 2017;2(2):263-9. https://doi.org/10.36387/jiis.v2i2.120

Maulidiyah NY. Uji Aktivitas Ekstrak Kloroform Daun Kenitu (Chrysophyllum cainito L.) Terhadap Penurunan Kadar Gula Darah Tikus Putih Jantan Galur Wistar (Rattus norvegicus L.) Yang Diinduksi Aloksan. Indonesia: Universitas Islam Negeri Maulana Malik Ibrahim; 2018.

Li B, Wang H, Zhou G, Zhang J, Su X, Huang Z, et al. VEGF-loaded biomimetic scaffolds: A promising approach to improve angiogenesis and osteogenesis in an ischemic environment. RSC Adv. 2017;7(8):4253-9. https://doi.org/10.1039/C6RA25294J DOI: https://doi.org/10.1039/C6RA25294J

Depari SA, Jihan D, Rambe A, Meilando R, Lisya C, Mutia MS, et al. The Effectiveness of Sunkist Orange Peel (Citrus sinensis (L.) Osbeck) Ethanol Extract on Blood Sugar Level of Streptozotocin Induced Wistar Rat (Rattus norvegicus) with Hypercholesterolemia. Biospecies. 2021;14(1):1-9.

Rianto L, Handayani IA, Septiyani A. Activity test of 96% ethanol extract of sugar apple seeds (Annona squamosa L.) as an anti-diarrhea caused by the bacterium Shigella dysenteriae using the disc diffusion method. J Ilm Manuntung. 2017;1(2):181-6. DOI: https://doi.org/10.51352/jim.v1i2.33

Monica B, Kurnia A, Prasetyo D. Effect of microemulsion preparations of karamunting leaf extract (Rhodomyrtus tomentosa [Aiton] hassk) and areca nut extract (Arace catechu Linnaeus) microemulsions on decreasing fasting blood sugar levels in male white rats induced by alloxan. J Borneo Cendekia. 2019;3:187-92. DOI: https://doi.org/10.54411/jbc.v3i2.149

Ludong RM, De Queljoe E, Simbala HE. Test the effectiveness of areca nut extract (Areca vestiaria) on reducing blood glucose levels in male white rats wistar strain (Rattus norvegicus) induced by alloxan. Pharmacon. 2019;8(2):416-25. https://doi.org/10.35799/pha.8.2019.29308 DOI: https://doi.org/10.35799/pha.8.2019.29308

Adha B, Febriani H. Hypoglycemic activity of Batak onion extract (Allium chinense g. Don.) on blood glucose levels of hyperglycemic white rats (Rattus norvegicus). Best J Biol Educ Sci Technol. 2021;4(2):49-55. DOI: https://doi.org/10.30743/best.v4i2.4258

Harahap E. Analisis Kadar Vitamin C Pada Pakkat (Calamus caesius Blume.) Secara Volumetri Dengan 2, 6-Diklorofenol Indofenol dan Iodimetri [Post Graduate Thesis, Universitas Sumatera Utara, Medan, Indonesia. (2017). Available from: https://repositori.usu.ac.id/handle/123456789/12910?show=full

Rompas IZ, Bodhi W, Lebang JS. Antihyperglycemic effectiveness test of ethanol extract of red gedi leaves (Abelmoschus manihot l. Medik) against alloxan-induced wistar rats (Rattus norvegicus). Pharmacon. 2021;10(3):940-7. https://doi.org/10.35799/pha.10.2021.35595

Tulung GL, Bodhi W, Siampa JP. The effectiveness Test of the ethanol extract of Centella asiatica (L.) Urban leaves as anti-diabetic against alloxan-induced male white rats (Rattus norvegicus). Pharmacon. 2021;10(1):736-42. https://doi.org/10.35799/pha.10.2021.32767 DOI: https://doi.org/10.35799/pha.10.2021.32767