Assessment of the Clinical Outcomes of Single Visit Regenerative Endodontic Procedure In Treating Necrotic Mature Teeth with Apical Periodontitis Using Biological Irrigating Solution
DOI:
https://doi.org/10.3889/oamjms.2023.11314Keywords:
Regenerative endodontics, Single visit regeneration, Propolis, Nano-propolis, Platelet-rich fibrinAbstract
Aim: to observe the effect of propolis and its nano counterpart when used as final irrigation materials and compare them to the conventionally used EDTA and saline on the clinical outcomes of necrotic mature anterior teeth with apical periodontitis after treating them using regenerative endodontic procedure.
Methodology: 84 patients who fulfilled the inclusion criteria were recruited for the study. Access cavity was prepared, rubber dam isolation was done. Mechanical preparation was performed up to #60 using K-files. Final irrigation procedure was done as follows:
Group A (Control ): Final irrigation was done using saline (3ml/min) followed by 17% EDTA solution (20 mL/canal, 5 min).
Group B (comparator 1): Final irrigation was done using propolis[1] irrigation (20 mL/canal, 5 min).
Group C (comparator 2): Final irrigation was done using Nano-propolis[2] irrigation (20 mL/canal, 5 min).
Bleeding was then induced, PRF was placed in the canals, followed by Collacote[3] and Bio-dentine[4] placement. Patients were recalled after 6 and 12 months. Teeth were checked for sensibility using electric pulp tester and thermal tests. All data was analysed using Kruskal Wallis test.
Results: All of the 84 patients (100%) within the 3 groups showed both clinical success and survival. Using Kruskal Wallis, there was no statistically significant difference among the three groups (p=1). Two teeth in the nano propolis group responded positively to thermal test at 12 months follow up. None of the teeth in the three tested groups (100%) responded to the
electric pulp test at 12 months. There was nostatistically significant difference between the three groups(p=1)
Conclusion: within the limitation of the study, single visit regenerative endodontics can be used to treat necrotic mature teeth with apical periodontitis and a periapical lesion.
Key words: Regenerative endodontics, single visit regeneration, propolis, Nano-propolis, Platelet-rich fibrin
[1] Propolis: Imtinan/Cairo, Egypt
[2] Nanopropolis: NanoTech/Cairo/Egypt
[3] Collacote: Collacote/Zimmer/Indiana /USA
[4] Biodentine: Acrostone/Cairo/Egypt
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Jung C, Kim S, Sun T, Cho YB, Song M. Pulp-dentin regeneration: Current approaches and challenges. J Tissue Eng. 2019;10:2041731418819263. https://doi.org/10.1177/2041731418819263 PMid:30728935 DOI: https://doi.org/10.1177/2041731418819263
Graziani F, Ivanovski S, Cei S, Ducci F, Tonetti M, Gabriele M. The in vitro effect of different PRP concentrations on osteoblasts and fibroblasts. Clin Oral Implants Res. 2006;17(2):212-9. https://doi.org/10.1111/j.1600-0501.2005.01203.x PMid:16584418 DOI: https://doi.org/10.1111/j.1600-0501.2005.01203.x
Narang I, Mittal N, Mishra N. A comparative evaluation of the blood clot, platelet-rich plasma, and platelet-rich fibrin in regeneration of necrotic immature permanent teeth: A clinical study. Contemp Clin Dent. 2015;6(1):63-8. https://doi.org/10.4103/0976-237X.149294 PMid:25684914 DOI: https://doi.org/10.4103/0976-237X.149294
Shivashankar VY, Johns DA, Maroli RK, Sekar M, Chandrasekaran R, Karthikeyan S, et al. Comparison of the Effect of PRP, PRF and Induced Bleeding in the revascularization of teeth with necrotic pulp and open apex: A triple blind randomized clinical trial. J Clin Diagn Res. 2017;11(6):ZC34-9. https://doi.org/10.7860/JCDR/2017/22352.10056 PMid:28765825 DOI: https://doi.org/10.7860/JCDR/2017/22352.10056
Elgendy AA, Fayyad DM. Cell viability and apoptotic changes of dental pulp stem cells treated with propolis, chitosan, and their nano counterparts. Tanta Dent J. 2017;14(4):198-207. https://doi.org/10.4103/tdj.tdj_27_17 DOI: https://doi.org/10.4103/tdj.tdj_27_17
Sena NT, Gomes BP, Vianna ME, Berber VB, Zaia AA, Ferraz CC, et al. In vitro antimicrobial activity of sodium hypochlorite and chlorhexidine against selected single-species biofilms. Int Endod J. 2006;39(11):878-85. https://doi.org/10.1111/j.1365-2591.2006.01161.x PMid:17014526 DOI: https://doi.org/10.1111/j.1365-2591.2006.01161.x
Bashetty K, Hegde J. Comparison of 2% chlorhexidine an 5.25% sodium hypochlorite irrigating solutions on postoperative pain: A randomized clinical trial. Indian J Dent Res. 2010;21(4):523-7. https://doi.org/10.4103/0970-9290.74225 PMid:21187618 DOI: https://doi.org/10.4103/0970-9290.74225
Bhardwaj A, Velmurugan N, Sumitha, Ballal S. Efficacy of passive ultrasonic irrigation with natural irrigants (Morinda citrifolia juice, aloe vera and Propolis) in comparison with 1% sodium hypochlorite for removal of E. faecalis biofilm: An in vitro study. Indian J Dent Res. 2013;24(1):35-41. https://doi.org/10.4103/0970-9290.114938 PMid:23852230 DOI: https://doi.org/10.4103/0970-9290.114938
Paryani K, Kim SG. Regenerative endodontic treatment of permanent teeth after completion of root development: A report of 2 cases. J Endod. 2013;39(7):929-34. https://doi.org/10.1016/j.joen.2013.04.029 PMid:23791266 DOI: https://doi.org/10.1016/j.joen.2013.04.029
El-Kateb NM, El-Backly RN, Amin WM, Abdalla AM. Quantitative assessment of intracanal regenerated tissues after regenerative endodontic procedures in mature teeth using magnetic resonance imaging: A randomized controlled clinical trial. J Endod. 2020;46(5):563-74. https://doi.org/10.1016/j.joen.2020.01.026 PMid:32173020 DOI: https://doi.org/10.1016/j.joen.2020.01.026
Topçuoğlu GM, Topçuoğlu HS. Regenerative endodontic therapy in a single visit using platelet-rich plasma and biodentine in necrotic and asymptomatic immature molar teeth: A report of 3 cases. J Endod. 2016;42(9):1344-6. https://doi.org/10.1016/j.joen.2016.06.005 PMid:27427186 DOI: https://doi.org/10.1016/j.joen.2016.06.005
Saoud TM, Martin G, Chen YH, Chen KL, Chen CA, Songtrakul K, et al. Treatment of mature permanent teeth with necrotic pulps and apical periodontitis using regenerative endodontic procedures: A case series. J Endod. 2016;42(1):57-65. https://doi.org/10.1016/j.joen.2015.09.015 PMid:26525552 DOI: https://doi.org/10.1016/j.joen.2015.09.015
Nageh M, Ahmed GM, El-Baz AA. Assessment of regaining pulp sensibility in mature necrotic teeth using a modified revascularization technique with platelet-rich fibrin: A clinical study. J Endod. 2018;44(10):1526-33. https://doi.org/10.1016/j.joen.2018.06.014 PMid:30174103 DOI: https://doi.org/10.1016/j.joen.2018.06.014
Abou Samra RA, El Backly RM, Aly HM, Nouh SR, Moussa SM. revascularization in mature permanent teeth with necrotic pulp and apical periodontitis: Case series. Alex Dent J. 2018;43(2):7-12. https://doi.org/10.21608/adjalexu.2018.57617 DOI: https://doi.org/10.21608/adjalexu.2018.57617
Arslan H, Ahmed H, Şahin Y, Yıldız ED, Gündoğdu EC, Güven Y, et al. Regenerative endodontic procedures in necrotic mature teeth with periapical radiolucencies: A preliminary randomized clinical study. J Endod. 2019;45(7):863-72. https://doi.org/10.1016/j.joen.2019.04.005 PMid:31155298 DOI: https://doi.org/10.1016/j.joen.2019.04.005
Kateb N, Fata MM. Influence of periapical lesion size on healing outcome following regenerative endodontic procedures: A clinical investigation. Oral Radiology. 2021;38(4):480-9. https://doi.org/10.1007/s11282-021-00578-8 PMid:34826060 DOI: https://doi.org/10.1007/s11282-021-00578-8
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Copyright (c) 2023 Maha Ahmed Nassar, Nehal Nabil Roshdy, Mohamed Kataia, Hadeel Mousa, Nihal Sabet (Author)
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