Studying the Specific (Adaptogenic and Anabolic) Activity of the Supramolecular Complex of 20-Hydroxyecdysone Triacetate with β-Cyclodextrin

B. I.  Tuleuov; M.  Kozhanova; B. S.  Temirgaziyev; Kairtay  Almagambetov; M. Z.  Shaidarov; R. B.  Seidakhmetova; P. B.  Drašar; S. M.  Adekenov

doi:10.3889/oamjms.2024.11906

Authors

B. I. Tuleuov JSC Research and Production Center “Phytochemistry,” Karaganda, Republic of Kazakhstan
M. Kozhanova JSC Research and Production Center “Phytochemistry,” Karaganda, Republic of Kazakhstan
B. S. Temirgaziyev JSC Research and Production Center “Phytochemistry,” Karaganda, Republic of Kazakhstan https://orcid.org/0000-0001-6994-3478
Kairtay Almagambetov JSC Research and Production Center “Phytochemistry,” Karaganda, Republic of Kazakhstan
M. Z. Shaidarov JSC Research and Production Center “Phytochemistry,” Karaganda, Republic of Kazakhstan
R. B. Seidakhmetova Laboratory of Chemistry of Steroid Compounds, Karaganda Medical University, Karaganda, Republic of Kazakhstan
P. B. Drašar Department of Chemistry of Natural Compounds, University of Chemistry and Technology, Prague, Czech Republic
S. M. Adekenov JSC Research and Production Center “Phytochemistry,” Karaganda, Republic of Kazakhstan

DOI:

https://doi.org/10.3889/oamjms.2024.11906

Keywords:

20-hydroxyecdysone triacetate, β-cyclodextrin, Supramolecular complex, Adaptogenic and anabolic activity

Abstract

BACKGROUND: A supramolecular complex of ecdysterone triacetate with β-cyclodextrin (β-CD) was synthesized to obtain new potentially bioactive substances and study the effect of bulky pharmacophore-functional groups on the preservation of the specific activity of 20-hydroxyecdysone. The adaptogenic and anabolic activities of the resulting water-soluble inclusion complex were studied. It was established that with the introduction of 3 acyl-pharmacophore groups in the form of encapsulated and hydrophilic supramolecular forms to experimental animals at a dose of 10 mg/kg, the studied complex has anabolic and adaptogenic effects, including increased stress resistance, increased physical endurance, as well as slightly increased weight of the heart and gonads compared to the control.

AIM: Study of the specific activity of the supramolecular complex of ecdysterone 3-acetate with β-CD.

METHODS: During the experimental study of the adaptogenic and anabolic effects of the supramolecular complex of ecdysterone 3-acetate with β-CD, the “open field” and “free swimming” tests were used. Statistical processing of the results was carried out using the Statistica 8 software package. Intergroup differences were assessed using the non-parametric Mann–Whitney U-test. For unpaired related groups, the non-parametric Wilcoxon test was used.

CONCLUSIONS: It has been established that physical activity (swimming) simultaneously has a significant stressor effect on animals and is characterized by an increased supply of glucose into the blood and the breakdown of blood proteins. It was revealed that the supramolecular complex of ecdysterone 3-acetate with β-CD at a dose of 10 mg/kg has anabolic and adaptogenic effects, in particular increased stress resistance and physical endurance of animals, as well as a slightly increased weight of the heart and gonads compared to the control.

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