Morphological Changes in the Hepatic Tissue at the Impact of Industrial Copper-bearing Dust in the Experiment

Khamida R. Abdikadirova; Kymbat Ye. Amreyeva; Saule B. Zhautikova; Olga A. Kostyleva; Fatima S. Abikenova; Bibigul T. Chergizova; Yulia P. Talaspekova; Saule Sh. Atshabarova

doi:10.3889/oamjms.2020.3473

Authors

Khamida R. Abdikadirova Department of Pathology, Non-Profit Joint-Stock Company “Karaganda Medical University”, Nutrition and Hygiene, Morphology and Physiology, Gogol 40, Karaganda 1000008, Kazakhstan
Kymbat Ye. Amreyeva Department of Pathology, Non-Profit Joint-Stock Company “Karaganda Medical University”, Nutrition and Hygiene, Morphology and Physiology, Gogol 40, Karaganda 1000008, Kazakhstan
Saule B. Zhautikova Department of Pathology, Non-Profit Joint-Stock Company “Karaganda Medical University”, Nutrition and Hygiene, Morphology and Physiology, Gogol 40, Karaganda 1000008, Kazakhstan
Olga A. Kostyleva Department of Pathology, Non-Profit Joint-Stock Company “Karaganda Medical University”, Nutrition and Hygiene, Morphology and Physiology, Gogol 40, Karaganda 1000008, Kazakhstan
Fatima S. Abikenova Department of Pathology, Non-Profit Joint-Stock Company “Karaganda Medical University”, Nutrition and Hygiene, Morphology and Physiology, Gogol 40, Karaganda 1000008, Kazakhstan
Bibigul T. Chergizova Department of Pathology, Non-Profit Joint-Stock Company “Karaganda Medical University”, Nutrition and Hygiene, Morphology and Physiology, Gogol 40, Karaganda 1000008, Kazakhstan
Yulia P. Talaspekova Department of Pathology, Non-Profit Joint-Stock Company “Karaganda Medical University”, Nutrition and Hygiene, Morphology and Physiology, Gogol 40, Karaganda 1000008, Kazakhstan
Saule Sh. Atshabarova Department of Pathology, Non-Profit Joint-Stock Company “Karaganda Medical University”, Nutrition and Hygiene, Morphology and Physiology, Gogol 40, Karaganda 1000008, Kazakhstan

DOI:

https://doi.org/10.3889/oamjms.2020.3473

Keywords:

Polymetallic dust, Copper, Liver, Hepatocyte, Morphology, Morphometry

Abstract

BACKGROUND: It is known that an increased intake of copper (Cu) has an adverse effect, and above all leads to the defeat of parenchymal organs, including liver tissue.

AIM: This study the morphological changes in the hepatic tissue at the impact of polymetallic Cu dust.

METHODS: An experimental study was carried out on the outbred white male rats. Dust was injected once intratracheally at a dose of 50 mg. For dynamic observation, the animals were killed in 1, 3, and 6 months with the control group using instant decapitation. The Balkhash industrial polymetallic dust with a predominant Cu content (Cu-0.6%) was used for the study. Morphological changes were assessed using histological and morphometric methods.

RESULTS: Morphometric examination of liver tissue at 30 days showed Vv necrosis increasing in 320 times in Group 2 (p < 0.001), Vv infiltrates – in 121 times (p < 0.001), Vv dystrophic altered hepatocytes – in 19.91 times (p < 0.001), Vv dual-core cells – in 23 times (p < 0.01), and Vv fibrosis – in 2.82 times (p < 0.001) in comparison with Group 1. Vv portal tracts are not reliably changed. In 90 days, there were also the following morphometric parameters increasing in comparison with the control group: Vv necrosis – in 522 times (p < 0.001), Vv infiltrates – in 395 times (p < 0.001), Vv dystrophic altered hepatocytes – in 26.7 times (p < 0.001), Vv dual-core cells – in 314 times (p < 0.01), and Vv fibrosis – in 13.27 times (p < 0.001). On the 180 day of the experiment, there was the increasing of Vv infiltrates in 421 times (p < 0.001), Vv dystrophic altered hepatocytes – in 34.09 times (p < 0.001), Vv dual-core cells – in 411 times (p < 0.001), and Vv fibrosis – in 54.09 times (p < 0.001)

CONCLUSION: The impact of polymetallic dust with 0.6% Cu concentration at the early stages leads to the changes in the liver in the form of reactive hepatitis with the following transformation into portal-type hepatitis.

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References

Guselnikov SR, Gogoleva OI, Lipatov GY, Adrianovsky VI, Samylkin AA. Professional morbidity of workers engaged in obtaining refined copper. Med Tr Prom Ekol. 2015;9:46-47.

Lozova EV, Karimova LK, Gainullina MK, Mavrina LN, Bagul NA, Salimgareeva TM. Hygienic assessment of working conditions of workers of mining and processing plants. Med Tr Prom Ekol. 2015;3:121-7.

Sadykov MN, Otarov EZ, Asenova LX, Makanova UK, et al. Assessment of working conditions of mining workers. Med Tr Prom Ekol. 2017;3:71-3.

Serebryakov PV, Kartashev OI, Fedina IN. Clinical and hygienic evaluation of health state of copper production workers in Far North. Med Tr Prom Ekol. 2016;1:25-8. PMid:27048139

Lipatov GY, Adrianovsky VI, Konstantinov VG, Gilyova YM. Mortality from malignant neoplasms of workers engaged in the enrichment of copper-containing ores. Ural Honey Zhur. 2010;1(66):5-7.

Izmerov NF, Kovalevsky EV, Rukavishnikov VS. Industrial dust. Aerosols predominantly fibrogenic action. In: Izmerov NF, Chuchalin AG, editors. Occupational Respiratory Diseases. Moscow, Russia: M: GEOTAR-Media; 2015. p. 44-58.

Kavasaki HA. Mechanistic review of silica-indused inhalation toxicity. Inhal Toxicol. 2015;27(8):363-77. PMid:26194035

Pankova VB. The Current problems of diagnostics and expertise of occupational diseases of the upper respiratory tract. Vestn Otorhinolaryngol. 2015;80(5):14-8. https://doi.org/10.17116/otorino201580514-18 PMid:26525465

Shorin SS. Environmental factors of industrial cities that worsen the health of the population, and ways to solve them. Vectnik Karagandinskogo univerciteta. Seria biologia. Medicina. Giografia. 2014;2:35-43.

Farshatova ER, Menshikova IA, Kamilov FK. The influence of the metals contained in copper-zinc sulfide ores, on bone metabolism. Med Vectnic Bashkortostan. 2014;4:57-9.

Pavlov VN, Bekmukhambetov EZ, Teregulova ZS, Mamyrbaev AA, Teregulov BF, Ishemgulov RR. The evaluation reproductive health of men living and working in conditions of mining technogenesis. Med Bull Bashkortostan. 2015;10(3):103-6.

Tataeva RK, Mussina AA, Burumbaeva MB. Morphological characteristics of liver tissue inhalation polymetallic aerosol condensation. News Natl Acad Sci Repub Kazakhstan Ser Biol Med. 2014;4(302):43-6.

Ibrayeva LK, Battakova JE, Amanbekova AU, Chanturia GR. Тhe character of energy changes in the activity of enzymes in the blood of rats under the action of finely dispersed aerosols of polymetallic dust and alimentary correction. Fundam Issled 2011;9(2):251-3.

Shaimardanova GM. Peroxidation in the lungs, liver and kidneys of rats with chronic exposure of polymetallic dust. Uspechy Sovremennogo Ectectvozhnania. 2008;8:19-22.

Kuz’mina LP, Izmerova NI, Burmistrova TB, Druzhinin VN. Patomorfoz sovremennyh form professional’nyh zabolevanij. Med Tu Prom Jekol. 2008;6:18-24.

Kupsha EI. Morphofunctional characteristic of the nuclei of hepatocytes of mice with lead intoxication. Mezhdunarodnyy Nauchno Issled Zhur. 2017;11:27-2.

Nasution RS, Yerizel E, Chaidir Z, Zein R. Protective effects of nothopanax scutellarium on hepatotoxicity of copper (II) induced to experimental. Open Access Maced J Med Sci 2020;8:283-6. https://doi.org/10.3889/oamjms.2020.4238

Pirs E. Histochemistry. Netherlands: Elsevier; 1962. p. 962.

Avtandilov GG. Morphometry in Pathology. Moscow: Meditsina; 1973. p. 152-171.