Phytochemical Screening, Gas Chromatography-mass Spectrometry Analysis, and Antidiabetic Effects of Corchorus olitorius Leaves in Rats

Heba M. I.  Abdallah; Gehad A. Abdel Jaleel; Hala Sh. Mohammed; Sawsan S. Mahmoud; Nemat A. Yassin; Amina  Gamal el Din; Nermeen M. Shaffie; Fatma A. Bassyouni

doi:10.3889/oamjms.2020.3796

Authors

Heba M. I. Abdallah Department of Pharmacology, Medical Research Division, National Research Centre (ID: 60014618), Giza, Egypt
Gehad A. Abdel Jaleel Department of Pharmacology, Medical Research Division, National Research Centre (ID: 60014618), Giza, Egypt
Hala Sh. Mohammed Department of Pharmacognosy, Faculty of Pharmacy (Girls), Al Azhar University, Cairo, Egypt
Sawsan S. Mahmoud Department of Pharmacology, Medical Research Division, National Research Centre (ID: 60014618), Giza, Egypt
Nemat A. Yassin Department of Pharmacology, Medical Research Division, National Research Centre (ID: 60014618), Giza, Egypt
Amina Gamal el Din Department of Pathology, Medical Research Division, National Research Centre (ID: 60014618), Giza, Egypt
Nermeen M. Shaffie Department of Pathology, Medical Research Division, National Research Centre (ID: 60014618), Giza, Egypt
Fatma A. Bassyouni Department of Chemistry of Natural and Microbial Products, Pharmaceutical Research and Drug Industries Division, National Research Centre (ID: 60014618), Giza, Egypt

DOI:

https://doi.org/10.3889/oamjms.2020.3796

Keywords:

Corchorus olitorius, Diabetes, Complications, Gas chromatography-mass spectrometry, Streptozotocin

Abstract

BACKGROUND: Therapies for diabetes mellitus are still meeting failure in most cases, especially in the developed stages of the disease due to incredible associating complications. Hence, there is a need for continuous development of curative therapies for that stubborn disease.

AIM: We aimed to investigate the antidiabetic effects of one of the most popular plants cultivated in Egypt, C. olitorius.

METHODS: Phytochemical screening of total alcoholic extract of Corchorus olitorius leaves and its aqueous and chloroform fractions revealed the presence of flavonoids, saponins, carbohydrates, tannins, coumarins, and alkaloids.

RESULTS: The gas chromatography-mass spectrometry analysis showed the presence of 12 and nine chemical compounds in aqueous and chloroform extracts, respectively. C. olitorius decreased serum glucose level and α-amylase activity. This effect was more pronounced in the total alcoholic extract and its chloroform fraction than the aqueous one. The extracts also adjusted the lipid profile, reduced liver injury parameters, and caused remarkable improvement and increase number, size, and density of functioning β-cells.

CONCLUSION: The findings suggest the antihyperglycemic and antioxidant effects of C. olitorius besides its beneficial effect on diabetic complications such as hyperlipidemia and liver injury. The presence of some phytochemicals such as theophylline, trans-2, 3-dimethoxycinnamic acid, 7-hydroxy-4-methyl coumarin, apigenin 7-glucoside, and glycitein may contribute to such pharmacological effects.

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