Tempuyung Leaves (Sonchus arvensis) Ameliorates Monosodium Urate Crystal-Induced Gouty Arthritis in Rats through Anti-Inflammatory Effects


  • Rachmat Hidayat Department of Biology, Faculty of Medicine, Universitas Sriwijaya, Palembang, Indonesia
  • Muhammad Reagan Department of Internal Medicine, Faculty of Medicine, Universitas Sriwijaya Palembang, Indonesia
  • Lusia Hayati Department of Biology, Faculty of Medicine, Universitas Sriwijaya, Palembang, Indonesia




Tempuyung, Sonchus arvensis, Monosodium Urate Crystal, Gouty Arthritis


BACKGROUND: Gouty arthritis, a chronic inflammatory disease characterized by severe pain and swelling in one or more synovial joints, as a result from joint deposition of monosodium urate (MSU) crystals. Tempuyung (Sonchus arvensis) is a plant that has been extensively studied in the role of shedding kidney stones and diuretics. It is presumed that it also has great potential in shedding MSU crystals in the joints.

AIM: This study focused on exploring the anti-inflammatory role of tempuyung extract (ET) on pro-inflammatory cytokines in gout arthritis white rats.

METHODS: The extraction of tempuyung was performed to obtain ET. A total of 30 Wistar rats were randomly divided into the following six groups, each containing five rats: Normal control group, MSU group (negative control), MSU + colchicine group (Col; 0.28 mg/kg), and MSU + ET group (at dose of 25 mg/kg, 50 mg/kg, and 100 mg/kg). Gouty arthritis was induced with 50 ml of MSU solution (20 mg/ml), which was injected into the left ankle joint cavity on day 7. Synovial fluid was evacuated for the examination of Western blotting of tumor necrosis factor-α (TNF-α). A portion of synovial tissue was fixed in 4% paraformaldehyde buffer for histopathological examination. Interleukin (IL)-1β levels in the synovial fluid of the joints were examined by IL-1β rat enzyme-linked immunosorbent assay. Statistical analysis was performed with way ANOVA followed by post hoc.

RESULTS: The histopathological image of the MSU model group showed a large number of inflammatory cells depicting an inflammatory reaction. This inflammation response decreased in the ET treatment group in dose-dependent manner. ET showed the effect of decreased pro-inflammatory cytokines expression in both IL-1β and TNF-α, as the dose increased.

CONCLUSION: Tempuyung extract possessed an anti-gout arthritis effect in white rats induced by MSU, by reducing the inflammatory response in the synovial joint.


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Kumar KE, Mastan SK, Reddy KR, Reddy GA, Raghunandan N, Chaitanya G. Antiarthrtic property of methanolic extract of Syzygium cumini seeds anti-arthritic property of the methanolic extract of Syzygium cumini seeds. Int J Interact Biol. 2008;4(1):55. https://doi.org/10.12980/jclm.1.2013c365

Sumarya IM, Adiputra N, Sukrama ID, Manuaba IB. Betel leaf extract (Piper betle L.) antihyperuricemia effect decreases oxidative stress by reducing the level of MDA and increase blood SOD levels of hyperuricemia Wistar rats (Rattus norvegicus). Bali Med J. 2016;5(2):2302-914. https://doi.org/10.15562/bmj. v5i2.218

Widyaningsih TD, Dwi W, Putri R, Murtini ES, Rochmawati N, Nangin D. Anti-inflammatory and anti-hyperuricemia properties of chicken feet cartilage : Treatment on gouty arthritis animal model. J Appl Pharm Sci. 2017;7(7):202-7. https://doi. org/10.7324/japs.2017.70729

Wang W, Bhole VM, Krishnan E. Chronic kidney disease as a risk factor for incident gout among men and women : Retrospective cohort study using data from the Framingham heart study. BMJ Open. 2015;5(4):e006843. https://doi. org/10.1136/bmjopen-2014-006843 PMid:25869687

Yang M, Teng G, Li G, Huang T, Xu R. Effectiveness of osthole on uric acid crystal-induced acute gouty arthritis through the inhibition of NLRP3 inflammasome. Int J Pharmacol. 2018;14(8):1169-78. https://doi.org/10.3923/ijp.2018.1169.1178

Partan RU, Hidayat R, Saleh MI, Parisa N, Tamzil NS. Effect of supplementation kayu manis (Cinnamomum burmannii) extract in neuronal cell death protection in Wistar rats lir-psychotic on haloperidol therapy. Asian J Pharm Clin Res. 2018;11(2):363-5. https://doi.org/10.22159/ajpcr.2018.v11i2.22024

Putri DP, Widyastuti Y, Dewi WS, Yunus A. The effect of shade and vermicompost application on yield and flavonoid levels of tempuyung (Sonchus arvensis). IOP Conf Series. 2018;142:012055. https://doi. org/10.1088/1755-1315/142/1/012055

Huang Q, Duan Z, Yang J, Ma X, Zhan R, Xu H, et al. SNP typing for Germplasm identification of Amomum villosum lour. Based on DNA barcoding markers. PLoS One. 2014;9(12):e114940. https://doi.org/10.1371/journal.pone.0114940 PMid:25531885

Jiang Y, You XY, Fu KL, Yin WL. Effects of extract from Mangifera indica leaf on monosodium urate crystal-induced gouty arthritis in rats. Evid Based Complement Alternat Med. 2012;2012:967573. https://doi.org/10.1155/2012/967573 PMid:23304232

Lee H, Choi HS, Park Y, Ahn CW, Jung SU, Park SH, et al. Effects of deer bone extract on the expression of pro-inflammatory cytokine and cartilage-related genes in monosodium iodoacetate-induced osteoarthritic rats. Biosci Biotechnol Biochem. 2014;78(10):1703-9. https://doi.org/10.10 80/09168451.2014.930317 PMid:25273135

Lee YM, Shon EJ, Kim OS, Kim DS. Effects of Mollugo pentaphylla extract on monosodium urate crystal-induced gouty arthritis in mice. BMC Complement Altern Med. 2017;17(1):447. https://doi.org/10.1186/s12906-017-1955-1 PMid:28874151

Zhang Z, Xu H, Li S, Zhang W, Shi S. Effects of several natural extracts on experimental gouty arthritis in rats. IPCBEE. 2012;39(2012):12-16. http://10.1155/2017/5852076

Nugraheni PW, Rahmawati F, Mahdi C, Prasetyawan S. Green tea extract (Camellia sinensis L.) effects on uric acid levels on hyperuricemia rats (Rattus norvegicus). J Pure Appl Chem Res. 2017;6(3):246-54. https://doi.org/10.21776/ ub.jpacr.2017.006.03.355

Park JE, Yeom Z, Park KT, Han EH, Yu HJ, Kang HS, et al. Hypouricemic effect of ethanol extract of Aster glehni leaves in potassium oxonate-induced hyperuricemic rats. Clin Nutr Res. 2018;7(2):126-35. https://doi.org/10.7762/cnr.2018.7.2.126 PMid:29713621

Schiltz C, Lioté F, Prudhommeaux F, Meunier A, Champy R, Callebert J, et al. Monosodium urate monohydrate crystal-induced inflammation in vivo quantitative histomorphometric analysis of cellular events. Arthritis Rheum. 2002;46(6):1643- 50. https://doi.org/10.1002/art.10326 PMid:12115197

Hameed BJ, Ramadhan UH. Xanthine oxidase inhibitory, antihyperuricemic, anti-inflammatory, antinociceptive activity of α-lipoic acid in gouty arthritis model. Asian J Pharm Clin Res. 2018;11(12):483-7. https://doi.org/10.22159/ajpcr.2018. v11i12.29160

Abu Bakar FI, Abu Bakar MF, Rahmat A, Abdullah N, Sabran SF, Endrini S. Anti-gout potential of Malaysian medicinal plants. Front Pharmacol. 2018;9:261. https://doi.org/10.3389/ fphar.2018.00261 PMid:29628890

Hsu DZ, Chen SJ, Chu PY, Liu MY. Therapeutic effects of sesame oil on monosodium urate crystal-induced acute inflammatory response in rats. Springerplus. 2013;2:659. https://doi.org/10.1186/2193-1801-2-659 PMid:24353977

Silva CR, Fröhlich JK, Oliveira SM, Cabreira TN, Rossato MF, Trevisan G, et al. The antinociceptive and anti-inflammatory effects of the crude extract of Jatropha isabellei in a rat gout model. J Ethnopharmacol. 2013;145(1):205-13. https://doi. org/10.1016/j.jep.2012.10.054 PMid:23127654

Partan RU, Hidayat R. The relationship between TNF-α gene polymorphism, pro-inflammatory cytokines and bone turnover markers in COPD patients with osteoporosis. J Phys. 2019;1246:012035. https://doi. org/10.1088/1742-6596/1246/1/012035

Moilanen LJ, Hämäläinen M, Lehtimäki L, Nieminen RM, Moilanen E. Urate crystal induced inflammation and joint pain are reduced in transient receptor potential ankyrin 1 deficient mice-potential role for transient receptor potential ankyrin 1 in gout. PLoS One. 2015;10(2):e0117770. https://doi.org/10.1371/ journal.pone.0117770 PMid:25658427

Li L, Teng M, Liu Y, Qu Y, Zhang Y, Lin F, et al. Anti-gouty arthritis and antihyperuricemia effects of sunflower (Helianthus annuus) head extract in gouty and hyperuricemia animal models. Biomed Res Int. 2017;2017:5852076. https://doi. org/10.1155/2017/5852076 PMid:28929115

Kou YY, Li YF, Xu M, Li WY, Yang M, Li RL. Effects of rupeng15 powder (RPP15) on monosodium urate crystal-induced gouty arthritis in rats. Evid Based Complement Altern Med. 2015;2015:527019. https://doi.org/10.1155/2015/527019

Han J, Xie Y, Sui F, Liu C, Du X, Liu C, et al. Zisheng shenqi decoction ameliorates monosodium urate crystal-induced gouty arthritis in rats through anti-inflammatory and anti-oxidative effects. Mol Med Rep. 2016;14(3):2589-97. https://doi. org/10.3892/mmr.2016.5526 PMid:27432278




How to Cite

Hidayat R, Reagan M, Hayati L. Tempuyung Leaves (Sonchus arvensis) Ameliorates Monosodium Urate Crystal-Induced Gouty Arthritis in Rats through Anti-Inflammatory Effects. Open Access Maced J Med Sci [Internet]. 2020 May 21 [cited 2022 Dec. 6];8(A):220-4. Available from: https://oamjms.eu/index.php/mjms/article/view/3801

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