Expression of Fibronectin and Tenascin after Direct Capping of the Pulp with Mineral Trioxide Aggregate and Biodentine®

Marina Eftimoska; Vasilka  Rendzova; Sonja Apostolska; Sasho Elencevski; Stevica Ristovska; Meri Pavlevska; Vesna  Filipovska-Micevska; Dusanka Stefanovic

doi:10.3889/oamjms.2020.3818

Authors

Marina Eftimoska Ss Cyril and Methodius University of Skopje, Faculty of Dentistry, Skopje, Republic of Macedonia
Vasilka Rendzova Ss Cyril and Methodius University of Skopje, Faculty of Dentistry, Skopje, Republic of Macedonia
Sonja Apostolska Ss Cyril and Methodius University of Skopje, Faculty of Dentistry, Skopje, Republic of Macedonia
Sasho Elencevski Ss Cyril and Methodius University of Skopje, Faculty of Dentistry, Skopje, Republic of Macedonia
Stevica Ristovska Ss Cyril and Methodius University of Skopje, Faculty of Dentistry, Skopje, Republic of Macedonia
Meri Pavlevska Ss Cyril and Methodius University of Skopje, Faculty of Dentistry, Skopje, Republic of Macedonia
Vesna Filipovska-Micevska Ss Cyril and Methodius University of Skopje, Faculty of Dentistry, Skopje, Republic of Macedonia
Dusanka Stefanovic Ss Cyril and Methodius University of Skopje, Faculty of Dentistry, Skopje, Republic of Macedonia

DOI:

https://doi.org/10.3889/oamjms.2020.3818

Keywords:

Caries, Remineralization, Fibronectin, Tenascin, Mineral trioxide aggregate, Biodentine®

Abstract

BACKGROUND: Caries is a destructive process leading to progressive demineralization of the inorganic part of the tooth, accompanied by enzyme disintegration of the organic component of the tooth tissue. Considering the fact that caries activity can be stopped if the demineralization process is stopped, it is clear that the objectives of caries treatment are focused toward elimination of etiological factors and stimulation of regeneration of the dental tissues. That is why in the last years treatment of the caries disease is targeted to remineralization of the initial carious lesions, as well as on the biological behavior of the pulp-dentine complex after application of certain medications.

AIM: The aim of this study is to evaluate impact of the two materials for direct capping, mineral trioxide aggregate (MTA) and Biodentine®, on the expression of glycoproteins fibronectin (FN), and tenascin (TN), responsible for dentinogenesis.

METHODS: In the tests were used materials MTA and Biodentine, as agents for direct capping of pulp exposure. From 60 extracted teeth included in this in vitro study, tissue cuts were made. Each of them was then analyzed on a light microscope to determine the amount of two extracellular matrix glycoproteins, FN-C, and TN-C.

RESULTS: Our study show that there is an expressed immunoreactivity for FN and for TN in the fibronectin bridge under MTA and Biodentine® after 8, and even after 30 days of their application.

CONCLUSION: Based on this study, we can conclude that both materials – MTA and Biodentine – may induce reparative dentinogenesis, in which FN and TN have certainly a major role in the formation of the fibronectin matrix.

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