Isolation of Amniotic Fluid Mesenchymal Stem Cells Obtained from Cesarean Sections


  • Bobby Indra Utama Doctoral Students of Biomedical Science, Faculty of Medicine, Andalas University, Padang, West Sumatera, Indonesia; Department of Obstetrics and Gynecology, Faculty of Medicine, General Hospital of Dr. M. Djamil, Padang, West Sumatera, Indonesia
  • Afriwardi Afriwardi Department of Physiology, Faculty of Medicine of Andalas University, Padang, West Sumatera, Indonesia
  • Budi Iman Santoso Department of Obstetrics and Gynecology, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia
  • Hirowati Ali Department of Biochemistry, Faculty of Medicine, Andalas University, Padang, West Sumatera, Indonesia



Amniotic fluid, Mesenchymal stem cells, Cesarean section


BACKGROUND: The term amniotic fluid (AF) can be an ideal alternative as a source of mesenchymal stem cells (MSCs), originating from the neonate. Preclinical studies of the second- and third-trimester amnion fluid cells confirmed the number of potential donors from this wasted material.

AIM: This study aims to look at the forming time of stem cells derived from AF-MSCs.

MATERIALS AND METHODS: AF samples from healthy human donors were collected during full-term C-sections and kept at 4°C until processed. The number of colony-forming unit-fibroblast was assessed microscopically by calculating spindle-shaped colonies that clearly resembled of fibroblasts and did not include colonies with rounded epithelioid morphology. The immunophenotyping of their independent AF preparations was done using the human MSC phenotyping kit which was done according to the manufacturer’s instruction by flow cytometry.

RESULTS: The result showed that it succeeded in getting 8 million cells which will be used for research on pelvic organ prolapse therapy using AF-MSCs. The stem cell isolation totally takes 6 weeks. We got 2 million stem cells in one flask.

CONCLUSION: This study concludes that the time needed for differentiation of AF-MSCs is 6 weeks and AF-MSCs express mesenchymal markers such as CD90, CD73 (SH3, SH), and CD105 (SH2) and these cells also express HLA antigens – ABC, CD 34, and CD 45 which are hematopoietic markers and endothelial CD31 markers.


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How to Cite

Utama BI, Afriwardi A, Santoso BI, Ali H. Isolation of Amniotic Fluid Mesenchymal Stem Cells Obtained from Cesarean Sections. Open Access Maced J Med Sci [Internet]. 2020 May 13 [cited 2023 Sep. 26];8(A):245-9. Available from:

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