The Effect of Human Bone Marrow Mesenchymal Stem Cells on Epidermal Growth Factor and Epidermal Growth Factor Receptor Expression in Re-epithelialization Process in the Healing of Burns on Experimental Rats

Authors

  • Gusti Revilla Department of Anatomy, Faculty of Medicine, Andalas University, Indonesia
  • Henny Mulyani Department of Anatomy, Faculty of Medicine, Andalas University, Padang, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2020.3959

Keywords:

Human bone marrow mesenchymal stem cells, Epidermal growth factor, Epidermal growth factor receptor, Healing of burn

Abstract

BACKGROUND: Research on human bone marrow mesenchymal stem cells (hBM-MSCs) for burns healing has been known to increase the percentage of integrin expression of α2β1, type I collagen, transforming growth factor-β, and matrix metalloproteinases-9, but research on giving hBM-MSCs to growth factor expression in the process of re-epithelialization of burn healing has not been done.

AIM: This study aims to the effect of hBM-MSCs given on the expression of epidermal growth factor (EGF) and EGF receptor (EGFR) in the process re-epithelialization in the healing of burn experimental rat.

MATERIALS AND METHODS: This research is experimental with the post-test only control design, using 30 Wistar rats. Rats were divided into two groups, namely, control (phosphate-buffered saline), and the treatment was given hBM-MSCs, and stem cells were given subcutaneous doses of 2 × 106 cells/ml. Before being treated rats were anesthetized using xylazine and ketamine then the rats were burned in the dorsal (spine) with full-thickness. On the 3, 7, and 14 days, skin tissue was taken to see the expression of EGF and EGFR by immunohistochemical methods. This study was approved by the Ethics Commission of the Faculty of Medicine, Andalas University, Padang. The results of the study were analyzed by the t-test.

RESULTS: Immunohistochemical examination of EGF and EGFR expressions after hBM-MSCs administration has significantly increased epithelialization compared with controls. Increased EGF expression was found on days 3 and 7 with moderate positive internal revenue service (IRS) assessment and on day 14 strong positive EGF expression, as well as EGFR expression on days 3 and 7 with moderate positive IRS assessment and on day 14 robust positive EGFR expression.

CONCLUSION: This study concluded that giving of hBM-MSCs can increase the expression of EGF and EGFR which enhances the process of re-epithelialization thereby accelerating the healing of burns of experimental rats.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Plum Analytics Artifact Widget Block

References

Pastar I, Stojadinovic O, Yin NC, Ramirez H, Nusbaum AG, Sawaya A, et al. Epithelialization in wound healing: A comprehensive review. Adv Wound Care. 2014;3(7):445-64. https://doi.org/10.1089/wound.2013.0473 PMid:25032064

Barrientos S, Olivera S, Michael SG, Harold B, Marjana TM. Growth factors and cytokines in wound healing. J Wound Repair Regen. 2008;16(5):585-601. https://doi. org/10.1111/j.1524-475x.2008.00410.x PMid:19128254

Metcalfe AD, Ferguson MW. Tissue engineering of replacement skin: The crossroads of biomaterials, wound healing, embryonic development, stem cells and regeneration. J R Soc Interface. 2007;4(14):413-37. https://doi.org/10.1098/rsif.2006.0179 PMid:17251138

Liu ZJ, Zhuge Y, Velazquez OC. Trafficking and differentiation of mesenchymal stem cells. J Cell Biochem. 2009;106(6):984-91. https://doi.org/10.1002/jcb.22091 PMid:19229871

Chen JS, Wongand VW, Gurtner GC. Therapeuticpotential of bone marrow-derived mesenchymal stem cells for cutaneous wound healing. Front Immunol. 2012;3:192. PMid:22787462

Semon JA, Nagy LH, Llamas CB, Tucker HA, Lee RH, Darwin J. Integrin expression and integrin-mediated adhesion in vitro of human multipotent stromal cells (MSCs) to endothelial cells from various blood vessels. Prockop Cell Tissue Res. 2010;341(1):147-58. https://doi.org/10.1007/ s00441-010-0994-4 PMid:20563599

Jeon YK, Jang YH, Yoo DR, Kim SN, Lee SK, Nam MJ. Mesenchymal stem cells’ interaction with skin: Wound-healing effect on fibroblast cells and skin tissue. Wound Rep Reg. 2010;18(6):655-61. https://doi.org/10.1111/j.1524-475x.2010.00636.x PMid:20955344

Sasaki M, Abe R, Fujita Y, Ando S, Inokuma D, Shimizu H. Mesenchymal stem cells are recruited into wounded skin and contribute to wound repair by transdifferentiation into multiple skin cell type. J Immunol. 2008;180(4):2581-7. https://doi. org/10.4049/jimmunol.180.4.2581 PMid:18250469

Gusti R, Darwin E, Yanwirasti Y, Rantam FA. Effect of allogeneic bone marrow-mesenchymal stem cells (BM-MSCs) to accelerate burn healing of rat on the expression of collagen Type I and integrin α2β1. Pak J Biol Sci 2016;19:345-51. https:// doi.org/10.3923/pjbs.2016.345.351 PMid:29023021

Yanwirasti Y, Gusti R, Nurafraininsyah N. Effect of BM-MSC on Serum MMP1 Levels on the Healing of Experimental Rat Burns. Beijing: APICA; 2013.

Kwon DS, Gao X, Liu YB, Dulchavsky DS, Danyluk AL, Bansal M, et al. Treatment with bone marrow-derived stromal cells accelerates wound healing in diabetic rats. Int Wound J 2008;5:453- 63. https://doi.org/10.1111/j.1742-481x.2007.00408.x PMid:18593394

Yeum CE, Park EY, Lee SB, Chun HJ, Chae GT. Quantification of MSCs involved in wound healing: Use of SIS to transfer MSCs to wound site and quantification of MSCs involved in skin wound healing. J Tissue Eng Regen Med. 2013;7(4):279-91. https://doi.org/10.1002/term.521 PMid:22278819

Dinh T, Braunagel S, Rosemblum BI. Growth factors in wound healing: The present and the future? Clin Pediatr Med Surg. 2015;32(1):109-19. PMid:25440422

Han S. Innovation and Advanced in Wound Healing. 2nd ed. Seoul: Seoul Republic of Korea; 2016.

Tamama K, Kawasaki H, Wells A. Epidermal growth factor (EGF) treatment on multipotential stromal cells (MSCs). Possible enhancement of therapeutic potential of MSC. J Biomed Biotechnol. 2009;2010:795385. https://doi.org/10.1155/2010/795385

Medina RJ, Kataoka K, Miyazaki M, Huh NH. Efficient differentiation into skin cells of bone marrow cells recovered in a pellet after density gradient fractionation. Int J Mol Med. 2006;17(5):721-7. https://doi.org/10.3892/ijmm.17.5.721 PMid:16596253

Wang G, Bunnell BA, Painter RG, Quiniones BC, Tom S, Lanson NA, et al. Adult stem cells from bone marrow stroma differentiate into airway epithelial cells: Potential therapy for cystic fibrosis. Proc Natl Acad Sci U S A. 2005;102(1):186-91. https://doi.org/10.1073/pnas.0406266102

Wu Y, Chen L, Scott PG, Tredget EE. Mesenchymal stem cells enhance wound healing through differentiation and angiogenesis. Stem Cells. 2007;25:2648-59. https://doi. org/10.1634/stemcells.2007-0226 PMid:17615264

Xue L, Xu YB, Xie JL, Tang JM, Shu B, Chen L, et al. Effects of human bone marrow mesenchymal stem cells on burn injury healing in a mouse model. Int J Clin Exp Pathol. 2013;6(7):1327-36. PMid:23826413

Lau K, Paus R, Tiede S, Day P, Bayat A. Exploring the role of stem cells in cutaneous wound healing. Exp Dermatol. 2009;18(11):921-33. https://doi.org/10.1111/j.1600-0625.2009.00942.x PMid:19719838

Downloads

Published

2020-06-10

How to Cite

1.
Revilla G, Mulyani H. The Effect of Human Bone Marrow Mesenchymal Stem Cells on Epidermal Growth Factor and Epidermal Growth Factor Receptor Expression in Re-epithelialization Process in the Healing of Burns on Experimental Rats. Open Access Maced J Med Sci [Internet]. 2020 Jun. 10 [cited 2024 Mar. 28];8(A):508-11. Available from: https://oamjms.eu/index.php/mjms/article/view/3959

Most read articles by the same author(s)